Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume explores various methodologies to study biochemical, molecular, and cellular biology aspects of some processes regulated by protein SUMOylation. SUMO: Methods and Protocols is organized into four parts, and starts with an historical overview on protein SUMOylation and a presentation of the methods included in the book. The first part also includes a review on chromatin regulation by dynamic SUMO modifications. The second part focuses on in vitro techniques, including biochemical methods to study mechanistic aspects of protein SUMOylation. The third part includes protocols to be used with cell cultures, which often are the first approaches used in most laboratories. The final part includes methodologies adapted for the analysis in vivo using distinct model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include a brief introduction to the subject, a list of necessary materials and reagents, a step-by-step reproducible laboratory protocol ending with a Notes section on troubleshooting tips, and tips and strategies to avoid known pitfalls. Unique and cutting-edge, SUMO: Methods and Protocols provides a comprehensive source of protocols for specialists and researchers not familiar with this vital system.

Biochemistry. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Ubiquitin Code aims to to be a useful practical guide to researches to help further their study in this field. .

Proteins. --- Post-translational modification. --- Post-translational Modifications.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This book, written by members of the European network PROTEOSTASIS, provides an up-to-date review of the research regarding protein homeostasis in health and disease. With new discoveries contributing to the increasing complexity of this topic, the book offers a detailed overview of the pathways regulating protein homeostasis, including autophagy and the ubiquitin protein family. Following a basic introduction, it explains how defects in protein homeostasis contribute to numerous pathologies, including cancer, neurodegeneration, inflammation and a number of rare diseases. In addition, it discusses, the role of protein homeostasis in cellular development and physiology. Highlighting the latest research in the field of protein homeostasis and its implications for various clinically relevant diseases, the book appeals to researchers and clinicians, while also offering a reference guide for scholars who are new to the field.

Proteomics. --- Molecular biology --- Proteins --- Proteasome Endopeptidase Complex. --- Proteostasis. --- Ubiquitin-Protein Ligases. --- E3 Ligase --- E3 Ubiquitin Ligase --- Ubiquitin Ligase E3 --- Ubiquitin-Protein Ligase E3 --- Ubiquitin-Protein Ligase --- Ligase E3, Ubiquitin --- Ligase E3, Ubiquitin-Protein --- Ligase, E3 --- Ligase, E3 Ubiquitin --- Ligase, Ubiquitin-Protein --- Ligases, Ubiquitin-Protein --- Ubiquitin Ligase, E3 --- Ubiquitin Protein Ligase --- Ubiquitin Protein Ligase E3 --- Ubiquitin Protein Ligases --- Protein Homeostasis --- Homeostases, Protein --- Homeostasis, Protein --- Protein Homeostases --- Proteostases --- Protein Folding --- Proteostasis Deficiencies --- 20S Proteasome --- Ingensin --- Macropain --- Macroxyproteinase --- Multicatalytic Endopeptidase Complex --- Multicatalytic Proteinase --- Prosome --- Proteasome --- Complex, Multicatalytic Endopeptidase --- Complex, Proteasome Endopeptidase --- Endopeptidase Complex, Multicatalytic --- Endopeptidase Complex, Proteasome --- Proteasome, 20S --- Proteinase, Multicatalytic --- Cell biology. --- Neurosciences. --- Immunology. --- Medicine. --- Cancer research. --- Cell Biology. --- Biomedicine, general. --- Cancer Research. --- Cancer research --- Health Workforce --- Immunobiology --- Life sciences --- Serology --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system --- Cell biology --- Cellular biology --- Biology --- Cells --- Homeòstasi --- Proteïnes --- Immunologia --- Malalties --- Malaltia --- Medicina --- Discapacitat física --- Malalties cardiovasculars --- Malalties constitucionals --- Malalties de l'aparell digestiu --- Malalties de l'aparell genital --- Malalties de l'aparell respiratori --- Malalties de la pell --- Malalties del sistema nerviós --- Malalties dels animals --- Malalties hematològiques --- Malalties hereditàries --- Malalties de l'aparell locomotor --- Malalties d'origen nutricional --- Malalties iatrogèniques --- Malalties immunitàries --- Malalties otorrinolaringològiques --- Malalties rares --- Oftalmopaties --- Serveis d'urgències mèdiques --- Síndromes --- Trastorns de la nutrició --- Trastorns del metabolisme --- Epidemiologia --- Biologia --- Complements (Immunitat) --- Immunofarmacologia --- Immunologia clínica --- Immunologia de la trasplantació --- Neuroimmunologia --- Resposta immunitària --- Sistema immunitari --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica --- Autorregulació (Fisiologia) --- Humors corporals --- Sistemes de control biològic --- Equilibri àcid-base --- Osmoregulació --- Cytology. --- Medicine—Research. --- Biology—Research. --- Cancer. --- Neuroscience. --- Biomedical Research. --- Cancer Biology. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors --- Tumors --- Proteïnes.

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Histology. Cytology --- Immunology. Immunopathology --- Oncology. Neoplasms --- Neuropathology --- Human medicine --- immunologie --- neurologie --- geneeskunde --- oncologie --- cytologie --- histologie