Listing 1 - 6 of 6 |
Sort by
|
Choose an application
The silencing of gene expression by small interfering RNAs has been recognized for only a relatively short time, but this has transformed our understanding of both transcriptional and post-transcriptional gene regulatory mechanisms. Multiple pathways culminate in formation of an RNA-induced silencing complex (RISC) containing a member of the Argonaute protein family bound to a 22-nt RNA strand that interacts with a target mRNA or gene through Watson-Crick base pairing. One consequence is mRNA-specific inhibition of protein synthesis. Evidence has been presented for diverse mechanisms, but there is not universal agreement in the field of how RISCs affect the translational machinery. The chapters collected in this volume represent contribution by leaders in the search to understand how miRNAs affect translation. They include chapters representing work in plants and Caenorhabditis elegans, the biological systems that originally led to the discovery of small interfering RNAs, but also include chapters on mammalian systems, with special emphasis on regulation of a key tumor suppressor and a protein that restricts human immunodeficiency virus 1 (HIV-1).
Genetic translation -- Regulation. --- Genetic translation. --- Genetic translation --- Non-coding RNA --- Gene silencing --- RNA Interference --- Physiology --- MicroRNAs --- Genetics --- Gene Expression Regulation --- RNA, Small Untranslated --- RNA, Antisense --- Biology --- Genetic Processes --- Gene Silencing --- Biological Science Disciplines --- Epigenesis, Genetic --- Genetic Phenomena --- Natural Science Disciplines --- Antisense Elements (Genetics) --- RNA --- RNA, Untranslated --- Nucleic Acids, Nucleotides, and Nucleosides --- Nucleic Acids --- Phenomena and Processes --- Disciplines and Occupations --- Chemicals and Drugs --- Biology - General --- Biophysics --- Biochemistry --- Chemistry --- Health & Biological Sciences --- Physical Sciences & Mathematics --- Regulation --- RNA. --- Gene silencing. --- Translation (Genetics) --- Gene inactivation --- Inactivation, Gene --- Silencing, Gene --- Ribonucleic acid --- Ribose nucleic acid --- Life sciences. --- Gene expression. --- Molecular biology. --- Biochemistry. --- Cell biology. --- Life Sciences. --- Biochemistry, general. --- Cell Biology. --- Gene Expression. --- Molecular Medicine. --- Genetic code --- Genetic regulation --- Nucleic acids --- Ribose --- Cytology. --- Medicine. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Genes --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Expression --- Composition --- Health Workforce --- Molecular biochemistry --- Molecular biophysics --- Biomolecules --- Systems biology --- Molecular genetics. --- Medicine --- Molecular Genetics. --- Biomedical Research. --- Research. --- Molecular biology --- Biological research --- Biomedical research
Choose an application
This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology. .
Human genetics. --- Human Genetics. --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology
Choose an application
Amino acids --- Cellular signal transduction. --- Genetic translation. --- Insulin --- Protein kinases. --- Proteins --- Proteins --- Physiological effect. --- Physiological effect. --- Metabolism --- Regulation. --- Synthesis.
Choose an application
Apoptosis. --- Calcium --- Cellular signal transduction. --- Genetic translation. --- Proteins --- Proteins --- Stress (Physiology). --- Physiological effect. --- Metabolism --- Regulation. --- Synthesis.
Choose an application
The silencing of gene expression by small interfering RNAs has been recognized for only a relatively short time, but this has transformed our understanding of both transcriptional and post-transcriptional gene regulatory mechanisms. Multiple pathways culminate in formation of an RNA-induced silencing complex (RISC) containing a member of the Argonaute protein family bound to a 22-nt RNA strand that interacts with a target mRNA or gene through Watson-Crick base pairing. One consequence is mRNA-specific inhibition of protein synthesis. Evidence has been presented for diverse mechanisms, but there is not universal agreement in the field of how RISCs affect the translational machinery. The chapters collected in this volume represent contribution by leaders in the search to understand how miRNAs affect translation. They include chapters representing work in plants and Caenorhabditis elegans, the biological systems that originally led to the discovery of small interfering RNAs, but also include chapters on mammalian systems, with special emphasis on regulation of a key tumor suppressor and a protein that restricts human immunodeficiency virus 1 (HIV-1).
Genetics --- Histology. Cytology --- General biochemistry --- Molecular biology --- biochemie --- cytologie --- histologie --- moleculaire biologie --- genexpressie
Choose an application
The silencing of gene expression by small interfering RNAs has been recognized for only a relatively short time, but this has transformed our understanding of both transcriptional and post-transcriptional gene regulatory mechanisms. Multiple pathways culminate in formation of an RNA-induced silencing complex (RISC) containing a member of the Argonaute protein family bound to a 22-nt RNA strand that interacts with a target mRNA or gene through Watson-Crick base pairing. One consequence is mRNA-specific inhibition of protein synthesis. Evidence has been presented for diverse mechanisms, but there is not universal agreement in the field of how RISCs affect the translational machinery. The chapters collected in this volume represent contribution by leaders in the search to understand how miRNAs affect translation. They include chapters representing work in plants and Caenorhabditis elegans, the biological systems that originally led to the discovery of small interfering RNAs, but also include chapters on mammalian systems, with special emphasis on regulation of a key tumor suppressor and a protein that restricts human immunodeficiency virus 1 (HIV-1).
Genetics --- Histology. Cytology --- General biochemistry --- Molecular biology --- biochemie --- cytologie --- histologie --- moleculaire biologie --- genexpressie
Listing 1 - 6 of 6 |
Sort by
|