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Currently, there is no single source that permits comparison of the factors, elements, enzymes and/or mechanisms employed by different classes of viruses for genome replication. As a result, we (and our students) often restrict our focus to our particular system, missing out on the opportunity to define unifying themes in viral genome replication or benefit from the advances in other systems. For example, extraordinary biological and experimental paradigms that have been established over the past five years for the DNA replication systems of bacteriophage T4 and T7 will likely be of great value to anyone interested in studying a replisome from any virus. These studies could easily go unnoticed by animal RNA and DNA virologists. It is our hope that this monograph will cross-fertilize and invigorate the field, as well as encourage students into this area of research.

Viral genomes. --- Viruses --Reproduction. --- Viral genomes --- Viruses --- Virus Replication --- Genetics --- Genome, Viral --- Virus Physiological Processes --- Biology --- Genome --- Microbiological Processes --- Virus Physiological Phenomena --- Biological Science Disciplines --- Genetic Structures --- Microbiological Phenomena --- Genetic Phenomena --- Natural Science Disciplines --- Disciplines and Occupations --- Phenomena and Processes --- Microbiology & Immunology --- Health & Biological Sciences --- Reproduction --- Reproduction. --- Replication of viruses --- Viral replication --- Viral genome --- Virus genomes --- Medicine. --- Human genetics. --- Immunology. --- Virology. --- Biochemistry. --- Cell biology. --- Microbiology. --- Biomedicine. --- Cell Biology. --- Biochemistry, general. --- Human Genetics. --- Microbial genomes --- Medical virology. --- Cytology. --- Heredity, Human --- Human biology --- Physical anthropology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Immunobiology --- Life sciences --- Serology --- Microbial biology --- Microorganisms --- Medical microbiology --- Virology --- Virus diseases --- Composition --- Microbiology

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This volume of 'Methods in Enzymology' focuses on the study of G-quadruplexes (G4) and i-motif structures within DNA, detailing various methodologies for investigating these complex nucleic acid formations. The book is edited by Kevin D. Raney, Robert L. Eoff, Alicia K. Byrd, and Samantha Kendrick, and it aims to provide comprehensive techniques and insights for researchers in molecular biology and biochemistry. It covers topics such as DNA helicase activity, protein-G4 interactions, and advanced imaging techniques. The volume serves as a valuable resource for scientists and researchers interested in understanding the biochemical and biophysical properties of G4 and i-motif structures, and their implications in genomic stability and transcription regulation.

Nucleic acids. --- DNA.