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"For better or worse, be it militarily, diplomatically, politically, economically, or culturally, Americans have had a profound role in shaping the wider world beyond them. Unsurprisingly, most non-Americans have passionate views about the nature of U.S. foreign policy. America has been a savior to some, a curse to others-and both have good reason to feel that way. And yet, such views are often also based on a caricature of American actions and intentions. For their part, Americans themselves have strong opinions about their role in the world and how it has evolved over time. Yet these views are shrouded as much in myth as they are grounded in fact. American Foreign Relations, then, suffers from being a subject of immense worldwide importance but almost complete misunderstanding; it provokes strong emotions and much debate in newspapers daily, but is accompanied by little comprehension. This Very Short Introduction aims to offer analysis of key events, episodes, crises, and individuals in the making of American foreign relations. It will discuss events such as the Revolutionary War, the Louisiana Purchase, the War of 1812, manifest destiny, the Mexican War, the Civil War, industrialization, the beginnings of globalization, the Spanish-American War, imperialism, the annexation of the Philippines, informal imperialism and the Open Door policy, World War I, isolationism, World War II, the Cold War from its origins to its end (including the Korean and Vietnam Wars), the Iraq Wars, 9/11, and Afghanistan. Such topics will be situated within an analytical narrative that follows chronology generally, but not strictly or comprehensively."

International relations. Foreign policy --- United States --- United States of America

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Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results. Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.

Plasmids --- Escherichia coli --- Genetic vectors --- Cloning vectors --- Expression vectors (Genetics) --- rDNA vectors --- Recombinant DNA vectors --- Vectors, Genetic --- Gene expression --- Molecular cloning --- Recombinant DNA --- E. coli (Bacterium) --- Escherichia --- Paragenes --- Cytoplasmic inheritance --- Mobile genetic elements --- Extrachromosomal DNA --- Biochemistry. --- Cytology. --- Biochemistry, general. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition

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