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Alpha-mannosidosis is an lysosomal storage disorder. These disorders are characterized by an enzyme deficiency and are responsible for the ineffective degradation of substances in the lysosomes. In alpha-mannosidosis the enzyme alpha-mannosidase works inadequate and causes intralysosomal storage of oligosaccharides. A lot of efforts have been made to provide an effective treatment. So far, enzyme replacement therapy seems very promising, however further research is necessary. Exercise therapy has been proven beneficial in other diseases. Research has shown significant effects of exercise therapy in Parkinson's and Huntington's disease. These diseases share motor function disturbances as a symptom with alpha-mannosidosis. Therefore it would be interesting to research the effects of exercise therapy in alpha-mannosidosis and investigate if it may be a worthy addition to treatment. To investigate the effects of exercise therapy, an exercise protocol was conducted on an alpha-mannosidosis mouse model and a control group. As exercise protocol a weekly routine of a 45 minutes training divided in three days on an alternating rotarod was administered. To evaluate the effect of the exercise protocol, a behavioral assessment was conducted. The rotarod showed no differences between genotype after a training period of three months which could be indicative of a beneficial effect of training. The balance beam test, which also measured motor coordination and balance, showed effects of genotype and exercise protocol. Knock-out mice were slower to traverse the beams and made more footslips. However exercised mice were faster to traverse than non-exercised mice. Reduced exploratory behavior in knock-out mice was found by means of the open field test and a difference in sensitivity to a psychostimulant was observed, which indicated a reduced sensitivity in knock-out mice. This finding is in contrast with what could have been expected, since previous research has shown psychotiform behavior in the alpha-mannosidosis mouse model. Further research is required to resolve this contradiction. No differences were found in working memory or in spatial learning and memory between knock-out and control mice. The latter is in contrast with previous research that show spatial memory impairment in knock-out mice. Limited effects of exercise protocol were found, nonetheless some effects are found and given the beneficial effects of more extensive exercise therapy in other diseases, further research with an elaborated exercise protocol is advised.