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Animal Cell Biotechnology: Methods and Protocols, Third Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterisation. Part II addresses tools for process and medium optimization as well as microcarrier technology while Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques. Part IV details cultivation techniques, and Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Third Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Biotechnology. --- Cell culture. --- Cell Culture. --- Cultures (Biology) --- Cytology --- Chemical engineering --- Genetic engineering --- Technique
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Animal Cell Biotechnology: Methods and Protocols, Second Edition constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and for optimizing critical parameters for cell culture considering the whole cascade from lab to final production. The volume is divided into five parts that reflect the processes required for different stages of production. In Part I, basic techniques for establishment of production cell lines are addressed, especially transduction techniques, cells for gene therapy and antibody production. Part II addresses basic cultivation techniques, such as microcarrier culture and encapsulation. Part III covers cell characterization and analysis, including flow cytometric applications, NMR-based techniques, and biochemical and cytometric techniques. Part IV details cultivation techniques, such as disposable bioreactors, hollow fiber cell culture, fixed bed reactors, and configuration of bioreactors. Part V covers downstream techniques such as membrane filtration techniques, while Part VI describes special applications, including retroviral vectors. Animal Cell Biotechnology: Methods and Protocols, Second Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Biotechnology. --- Cytology. --- Animal genetics. --- Toxicology. --- Cell Biology. --- Animal Genetics and Genomics. --- Pharmacology/Toxicology. --- Chemicals --- Medicine --- Pharmacology --- Poisoning --- Poisons --- Genetics --- Cell biology --- Cellular biology --- Biology --- Cells --- Chemical engineering --- Genetic engineering --- Toxicology
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Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.
Animal cell biotechnology --- Animal cells in biotechnology --- Animal biotechnology --- Biotechnology. --- Pharmaceutical chemistry. --- Pharmaceutics. --- Chemistry, Medical and pharmaceutical --- Chemistry, Pharmaceutical --- Drug chemistry --- Drugs --- Medical chemistry --- Medicinal chemistry --- Pharmacochemistry --- Chemistry --- Chemical engineering --- Genetic engineering
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This volume “Cell Engineerring 11 - Biopharmaceutical Manufacturing: Progress, Trends and Challenges” is a source of the latest innovative research and technical development in biomanufacturing systems. It is organised into 2 parts: 1) Manufacturing of recombinant therapeutic proteins (e.g. therapeutic antibodies, biosimilars/biogenerics) and 2) Manufacturing aspects of cell and gene therapy. Each with selected chapters on the following topics for both up- and downstream, such as: Advanced process strategies, especially continuous manufacturing, Advanced culture techniques, especially single-use systems, Process transfer, scale-up/scale-down models, Processing advances/Manufacturing productivity/efficiency, Model-assisted process understanding and development/Digital Twins, Process controls and analytics, Quality control, Quality by design, Facility design and full-scale commercial systems, manufacturing technology innovation. The book comprises contributions of experts from academia and industry active in the field of cell culture development for the production of recombinant proteins, cell therapy and gene therapy, with consideration of Digital Twin´s and facility design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. Inevitably, some omissions will occur in the test, but the authors have sought to avoid duplications by extensive cross-referencing to chapters in other volumes of this series and elsewhere. We hope the volume provides a useful compendium of techniques for scientists in industrial and research laboratories active in this field.
Cytology. --- Biomedical engineering. --- Biotechnology. --- Biochemical engineering. --- Pharmaceutical chemistry. --- Vaccines --- Biomaterials. --- Cell Biology. --- Biomedical Engineering and Bioengineering. --- Bioprocess Engineering. --- Pharmaceutics. --- Biomaterials-Vaccines.
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This contributed volume is dedicated towards the progress achieved within the last years in all areas of Cell Culture Engineering and Technology. It comprises contributions of active researchers in the field of cell culture development for the production of recombinant proteins, cell line development, cell therapy and gene therapy, with consideration of media development, process scale-up, reactor design, monitoring and control and model-assisted strategies for process design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. This book is conceived for graduate students, postdoctoral fellows and researchers interested in the latest developments in Cell Engineering.
Biological techniques --- Histology. Cytology --- Human biochemistry --- Pharmacology. Therapy --- Pathological biochemistry --- Human medicine --- Biotechnology --- medische biochemie --- biologische technieken --- bio-engineering --- biomedische wetenschappen --- geneeskunde --- biotechnologie --- cytologie --- histologie --- Cell culture. --- Cell culture --- Cultures (Biology) --- Cytology --- Technique --- Medicine --- Biology --- Cytology. --- Regenerative medicine. --- Biomedical engineering. --- Biomedical Research. --- Cell Biology. --- Biological Techniques. --- Regenerative Medicine and Tissue Engineering. --- Biomedical Engineering and Bioengineering. --- Research. --- Technique. --- Cell biology --- Cellular biology --- Cells --- Biological research --- Biomedical research --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Health Workforce --- Regeneration (Biology)
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J. S. Hayes E. M. Czekanska R. G. Richards The Cell-Surface Interaction Stefanie Michaelis Rudolf Robelek achim Wegener Studying Cell-Surface Interactions In Vitro: A Survey of Experimental Approaches and Techniques Kyobum Kim Diana M. Yoon Antonios G. Mikos F. Kurtis Kasper Harnessing Cell-Biomaterial Interactions for Osteochondral Tissue Regeneration Mathias Wilhelmi Bettina Giere Michael Harder Interaction of Cells with Decellularized Biological Materials Arie Bruinink Reto Luginbuehl Evaluation of Biocompatibility Using In Vitro Methods: Interpretation and Limitations Margit Schulze Edda Tobiasch Artificial Scaffolds and Mesenchymal Stem Cells for Hard Tissues Julia Will Lutz-Christian Gerhardt Aldo R. Boccaccini Bioactive Glass-Based Scaffolds for Bone Tissue Engineering Tali Re’em Smadar Cohen Microenvironment Design for Stem Cell Fate Determination Sonja Kress Anne Neumann Birgit Weyand Cornelia Kasper Stem Cell Differentiation Depending on Different Surfaces Frank Witte Ivonne Bartsch Elmar Willbold Designing the Biocompatibility of Biohybrids K. Wiegandt C. Goepfert R. Pörtner R. Janssen Interaction of Cartilage and Ceramic Matrix Debarun Das Ziyang Zhang Thomas Winkler Meenakshi Mour Christina I. Günter Michael M. Morlock Hans-Günther Machens Arndt F. Schilling Bioresorption and Degradation of Biomaterials.
Cell Physiological Processes --- Culture Techniques --- Clinical Laboratory Techniques --- Cell Physiological Phenomena --- Phenomena and Processes --- Investigative Techniques --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Cell Communication --- Cell Culture Techniques --- Tissue Engineering --- Mechanical Engineering --- Engineering & Applied Sciences --- Bioengineering --- Tissue engineering. --- Cell culture. --- Chemistry. --- Biotechnology. --- Cell biology. --- Biomaterials. --- Cell Biology. --- Biocompatible materials --- Biomaterials --- Medical materials --- Medicine --- Biomedical engineering --- Materials --- Biocompatibility --- Prosthesis --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Chemical engineering --- Genetic engineering --- Physical sciences --- Cultures (Biology) --- Cytology --- Regenerative medicine --- Tissue culture --- Technique --- Cytology. --- Bioartificial materials --- Hemocompatible materials --- Biomaterials (Biomedical materials)
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