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Wheat RFLP markers were selected for providing a readable hybridization pattern and characterized for their polymorphism information content. We report the mapping of 160 loci, corresponding to 132 markers, on the genetic map of a wheat reference population. This map resulting from joined works of different labs, constitutes an essential tool for further molecular marker assisted wheat breeding. Molecular markers were used to study the genetic basis of the quantitative resistance to powdery mildew of the wheat line RE714. Two doubled haploid populations from the crosses between RE714 and the susceptible parents Festin and Hardi were used. Using RFLP and microsatellites markers, genetic maps were developed for both populations ; line resistance was observed in field tests over different environments. Considering both populations and all the environments tested, the segregation data from molecular markers and resistance allowed us to detect 9 QTL (Quantitative Trait Loci) involved in the quantitative resistance of RE714. Among them, we found residual effects of the race-specific resistance genes Pm4b and MIRE. Two major QTL were very stable since they were detected across the two populations and all environments tested. Comparing the two populations, we show differences in the QTL detected according to the susceptible parent used and that the genetic background of Hardi leads to a lack of residual effect at the Pm4b gene.

Triticum aestivum --- Mildews --- Disease resistance --- genomes --- genetic markers --- genetic maps --- Genetic polymorphism --- RFLP