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Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma.  Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.       Authoritative and practical Astrocytes: Methods and Protocols seeks to aid scientists and researchers looking to use more specialized techniques to examine astrocyte function. .

Neurosciences. --- Human physiology. --- Human Physiology. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system --- Human biology --- Physiology --- Human body

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Providing a selection of the key techniques that are used in characterizing cerebral angiogenesis, Cerebral Angiogenesis: Methods and Protocols aims to define the cellular and molecular mechanisms underlying this important process.  Divided into six parts, this detailed volume examines cerebral angiogenesis occurring in different scenarios, a variety of different models in which cerebral angiogenesis can be studied, methods to characterize and quantify angiogenic events as well as several different approaches to measure changes in cerebral blood flow, different approaches to investigate the role of specific candidate genes in cerebral angiogenesis, methods of therapeutically manipulating cerebral angiogenesis by gene delivery, and finally important approaches to examine cerebral angiogenic mechanisms in vitro.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their specific topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Comprehensive and decidedly practical, Cerebral Angiogenesis: Methods and Protocols should be a vital resource for all researchers, both those new to this field as well as those looking to use more specialized and sophisticated techniques to examine blood vessel growth in the brain.

Neurosciences. --- Neurobiology. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system --- Neurosciences

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