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Dissertation
Exploring effects of climate change on soil organic matter dynamics at the soil profile scale.
Authors: --- --- ---
ISBN: 9789088261480 Year: 2010 Publisher: Leuven K.U.Leuven. Faculteit Bio-ingenieurswetenschappen

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Sinds het begin van het Industriële tijdperk in de 18de eeuw is de CO2-c oncentratie in de atmosfeer gestegen van 280 ppm tot 388 ppm, wat een to ename met bijna 40% betekent. Het versterkte broeikaseffect is voor 70% toe te wijzen aan deze antropogene stijging van het CO2 gehalte, met als gevolg een verandering van het klimaat. Deze klimaatverandering uit zic h in een stijging van de temperatuur en van het zeeniveau, in de verande ring van neerslagpatronen en in een toegenomen frequentie van extreme dr oogte of overstromingen. Jaarlijks wordt ongeveer 60 Gt koolstof (C) uit gewisseld tussen de atmosfeer en terrestrische ecosystemen. Deze stroom wordt aangedreven door de natuurlijke cyclus van fotosynthese en microbi ële afbraak van organische stof in de bodem. De balans tussen deze twee processen wordt beïnvloed door het klimaat. Zowel de snelheid van fotosy nthese, dat voor de aanvoer van C naar de bodem zorgt, als van microbiël e afbraak van bodem organische stof (BOS), dat C terug omzet in CO2, nem en toe met de temperatuur. BOS afbraak stijgt over het algemeen echter s neller met de temperatuur dan fotosynthese, wat ervoor zorgt dat klimaat verandering een netto toename

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Dissertation
Farmer & researcher collaboration for pro-poor development, through soybean production : a case study in SW-Kenya.
Authors: --- --- ---
ISBN: 9789088261336 Year: 2010 Publisher: Leuven K.U.Leuven. Arenberg doctoraatsschool wetenschap & technologie

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Arme boeren in Sub-Sahara Afrika zitten vast in een vicieuze armoede cir kel omdat ze niet over de nodige middelen beschikken om voldoende te kun nen investeren in hun velden. Velen onder hen planten jaar in jaar uit z onder bemesting waardoor niet alleen hun oogsten mager zijn, maar ze hun bodems ook gradueel uitputten. Het Tropische Instituut voor bodembiolog ie en bodemvruchtbaarheid (TSBF-CIAT) introduceerde sojabonen in de regi o rond Migori (Zuid West Kenia) in 2005 om de levensconditie van arme bo eren te verbeteren. De dubbel-doel promiscue variëteiten die gebruikt we rden produceren niet alleen sojabonen voor een goede voeding en bron van inkomen maar kunnen ook de bodemvruchtbaarheid ten goede komen. Ze gebr uiken tijdens hun groei stikstof uit de lucht (i.p.v uit de bodems) en p roduceren zo grote hoeveelheden stikstofrijke bladeren die na de groei v an de plant op de bodem vallen. Zo verbeteren ze de kwaliteit van de bod ems wat een impact heeft op het volgend gewas in rotatie. Onder goede kl imaatsomstandigheden, voldoende bemesting en goed onderhoud van de velde n kunnen deze sojaplanten de volgende maïsoogst zelfs verdubbelen. Het probleem is dat arme boeren de middelen niet hebben om soja op een o ptimale manier te kweken. Het doel van dit onderzoek was om te analysere n of en hoe: 1) sojaproductie toegankelijk kan zijn voor arme boeren; en 2) de drie voordelen van sojaproductie (bodemvruchtbaarheid, voeding, i nkomen) tot uiting kunnen komen ondanks de suboptimale sojaproductiemeth odes. We analyseren tenslotte 3) de rol van samenwerking tussen boeren e n onderzoekers in het aanpassen van landbouw aan de situatie van arme bo eren. Dit onderzoek vond plaats tussen 2006 en 2008. 1) SOJAPRODUCTIE CONTEXTUALISEREN. De regio rond Migori heeft voldoende oppervlakte landbouwgrond, twee regenseizoenen per jaar, redelijke bodem s en de bevolking heeft een relatief hoog onderwijsniveau. Ondanks dit a lles leeft 49% van de bevolking in armoede (Hoofdstuk 2). De grootste be perkingen voor landbouw in de regio zijn: 1) gebrekkige toegang tot sted en, markten en goederen; 2) gebrekkige bemesting van de velden als resul taat van geldtekort; 3) tekort aan landbouw werkkrachten. Om de werkdruk en kost van meststoffen in sojaproductie te verminderen, hebben we coll aboratieve experimenten opgezet (Hoofdstuk 3). We ondervonden dat sojapl anten met lokale meststoffen (houtassen en koeienmest) evenveel konden p roduceren dan met minerale meststoffen (di-ammonium fosfaat). We bespaar den 1/3de van de planttijd door soja in geulen te planten in plaats van in individuele gaten zonder impact op de oogst. Door zaden uit te strooi en, bespaarden we 89% van de planttijd en verloren we slechts -15% oogst . Door slechts één keer onkruid te wieden in plaats van twee, bespaarden we 36% van de totale werktijd en verminderde de oogst slechts met -5%. De tijdsbesparing die deze plantmethodes opleveren kan vervolgens meer n uttig besteed worden om meststoffen te verzamelen. Deze resultaten beves tigen dat de productie van soja dus toegankelijk kan zijn voor arme boer en. 2) TOEGANKELIJKHEID VAN DE VOORDELEN VAN SOJA. Door maïs te planten na d e soja-experimenten konden we aantonen dat rotatie effecten tot uiting k omen, zelfs met suboptimale sojaproductiemethodes (Hoofdstuk 4). Na twee rotatiecycli bekwamen we zonder gebruik van meststoffen 90% meer maïsoo gst dan in een veld dat vier seizoenen met continue maïs beplant was. Ee n boer die het gewoon is maïs zonder meststoffen te planten kan dus via soja-maïs rotatie evenveel maïs bekomen in één seizoen dan wat hij norma al in twee seizoenen oogst. De soja oogst is dan een netto voordeel van het systeem. We ondervonden ook dat arme boeren sojavoedingsproducten ku nnen gebruiken als vervanging voor dure dierlijke producten (Hoofdstuk 5 ). In verband met het vermarkten van sojabonen merkten we op dat een kle inschalige arme boer een hogere productiekost heeft (0.40 US$ kg-1) dan de prijs die de Keniaanse olieindustrie in 2008 bood (0.31 US$ kg-1) (Ho ofdstuk 5). Sojaproducten die thuis kunnen bereid worden, zoals thee, no ten, melk, enz. kunnen echter verkocht worden aan de hogere prijs van 2. 10-3.54 US$ kg-1 (of een inkomen van 1190-2030 US$ ha-1). Deze producten kunnen een interessante bron van inkomen vormen voor vrouwen. Dit wijst op het belang van vormingsmateriaal dat leesbaar is door geletterde maa r ook ongeletterde vrouwen om zo deze vorm van inkomen toegankelijk te m aken voor de armste boeren. Boeren die meer financiële middelen hebben k unnen investeren in technologieën om de productiviteit van hun soja te v erhogen en zo aan een lagere kost te kunnen produceren. Dit geeft hen to egang tot grotere markten via de sojacoöperatieve of de industrie. 3) SAMENWERKING TUSSEN BOEREN EN ONDERZOEKERS. We vergeleken twee niveau s van samenwerking met boeren: de collaboratieve experimenten (CE) en de experimenten van boeren (FE). In de vier CE zones, hebben we de experim enten samen met de boeren ontworpen en geleid. De aanpak bleek efficiënt voor het identificeren van gepaste experimentele problemen en experimen tele behandelingen (Hoofdstuk 6). De samenwerking met de boeren liet ons toe om de behandelingen die niet adopteerbaar zijn voor arme boeren uit de experimenten weg te filteren zodat de behandelingen steeds beter pas ten op de lokale levencondities in de regio. De CE gaf ook een kans aan boeren om behandelingen die “archaïsch” lijken in een formeel kader te t esten zonder risico op commentaar van buren (indien ze die in hun eigen veld hadden getest). De samenwerking leidde dus tot meer aangepaste expe rimenten en tot meer aangepaste productiemethodes voor soja. In de FE zo nes, gaven we de boeren de basistraining rond het opzetten van hun eigen experimenten (Hoofdstuk 7). De experimenten van de boeren bevatten vele behandelingen die een bron kunnen zijn van inspiratie voor latere exper imenten. Tussen 2006 en 2008 vergrootte de diversiteit aan behandelingen in de sojavelden van boeren. Boeren bleven ook verder experimenteren. B oeren uit CE en FE zones hadden behandelingen uit de CE experimenten gea dopteerd in hun eigen velden, ondanks het feit dat de FE boeren nooit di rect contact hadden met de CE experimenten. We hadden hen enkel de oogst resultaten van de CE meegedeeld. We concludeerden dat de FE aanpak ertoe had geleid dat boeren meer vatbaar werden voor informatie van buitenuit en deze gingen testen in hun eigen velden. De aanpak had dus een kanaal gevormd voor het snel verspreiden van de resultaten van de CE. Tenslott e analyseerden we de evolutie van de relatie tussen boeren en onderzoeke rs tijdens de loop van het project tussen 2006 en 2008 (Hoofdstuk 8) en onderscheidden we hierin drie verschillende fases. 1) In de initiële fas e waren de boeren heel enthousiast, maar dit enthousiasme was gebaseerd op onrealistische verwachtingen; 2) hierop volgde een fase van teleurste llingen, waardoor de interesse van boeren voor het project verminderde; 3) in de derde fase onderging het project een nieuwe heropleving, gebase erd op nieuwe maar meer realistische verwachtingen. De negatieve effecte n hadden hun oorsprong in interculturele verschillen, vooroordelen en co mmunicatieproblemen. CONCLUSIES. We besluiten uit dit werk (Hoofdstuk 9) dat sojaproductie en zijn drie potentiële voordelen toegankelijk kunnen zijn voor arme boere n indien onderzoekers de nadruk leggen op technologieën die in hun conte xt toegankelijk zijn (onder andere lokale meststoffen, werkdruk verminde rende technieken, lokale recepten en lokale marktopties). Deze lokale op lossingen kunnen arme boeren de kans geven om gradueel hun inkomen te ve rhogen en zo geleidelijk aan over te schakelen naar meer productieve met hodes. We benadrukken ook het belang van “samenwerking tussen boeren en onderzoekers” om landbouwonderzoek beter te kunnen richten naar de omsta ndigheden van de boeren; “ontwikkeling met een focus op de armsten”. Om zulke samenwerking succesvol te maken moeten onderzoekers bereid zijn fr equent in contact te zijn met de boeren en de hiërarchie tussen hen en b oeren geleidelijk aan af te breken.

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Dissertation
Diagnosing cassava’s nutritional needs: a key step in the development of site-specific fertilizer recommendations for cassava growers in Tanzania

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Cassava (Manihot esculenta Crantz) is a root crop grown all over the tropics, being the staple food for almost 800 million people. Part of its popularity is due to the fact that it can grow on marginal soils under dry conditions. Yields in Tanzania average around 5 tonnes fresh roots per hectare far below its potentials. Increasing interest in cassava starch by various industries is now demanding higher production, hence better yields. One solution to these low yields is balancing the nutrient supply by making site-specific fertilizer recommendations. Therefore, the nutritional needs of the crop must be known. This study is focussing on the different methods that have been used to assess a plant’s nutritional needs based on leaf tissue analysis. The youngest fully expanded cassava leaves from plots with different fertilizer applications were cut and analysed for nutrient contents. After this, classical methods of sufficiency ranges and critical values are compared to newer and now widely applied methods such as the Diagnosis and Recommendation Integrated System (DRIS) and Compositional Nutrient Diagnosis (CND) together with the less used methods of Deviation from Optimum Percentage (DOP) and Plant Analysis with Standardized Scores (PASS). All methods rely on reference concentrations found by splitting a sample population in a high-yielding and low-yielding population. Methods to split the populations were compared for their robustness to small datasets and the mean yield plus 4/3rd of the standard deviation seemed the best option. A yield cut-off of 40.38 tonnes fresh roots per hectare was used. All methods pointed towards deficiencies of zinc, boron and potassium and an excess in manganese. After linear regression, DRIS and CND compared very well to each other (R2adj = 0.71), while PASS had little in common with DRIS (R2adj = 0.23) and CND (R2adj = 0.39). Performance of the methods with regards to yields could not be evaluated with the available dataset. An exploratory study was also done to inspect the possibility to use carbon isotope discrimination as a tool to guide efforts towards yield improvement in situations where drought is determining yields. Two on-station trials without fertilizing and five on-farm trials with different fertilizer treatments were established. Shoots of cassava plants were tagged and newly grown shoot tissue was cut after two weeks. Carbon signatures (δ13C) of these cuttings were measured. Clear variations in carbon signatures were observed. Within-field variation in the on-station trials was substantive and reaching over 1 ‰. It is clear that many factors could play a role. Controlled experiments with varying irrigation regimes, multiple varieties at various growing stages and different fertilizer treatments could give more insights in carbon signature variations.

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Dissertation
The effect of conservation agriculture practices on soil quality, yield and profitability in maize-based production systems in Mexico
Authors: --- ---
Year: 2017 Publisher: Leuven KU Leuven. Faculteit Bio-ingenieurswetenschappen

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Conservation agriculture, based on the combined implementation of (i) minimal soil disturbance, (ii) soil cover by crop residue retention or cover crops and (iii) crop diversification, has been identified as a sustainable improvement of conventional agriculture practices. It can decrease soil degradation and erosion, improve water conservation, enhance soil life, improve labour efficiency and decrease fossil fuel dependence. Despite these benefits, adoption of conservation agriculture among smallholder farmers has remained limited. The International Maize and Wheat Improvement Centre (CIMMYT) is investigating and disseminating conservation agriculture practices under small and medium scale maize based production systems in Mexico. In this research, eight experimental platforms were examined to evaluate the sustainability of conservation and conventional agriculture practices, based on the evaluation of the diagnostic criteria (i) soil quality, (ii) yield and (iii) profitability. The platforms are linked to CIMMYT’s conservation agriculture program and located in central and south Mexico. Physical soil parameters that were assessed are indicative for direct infiltration, aggregate distribution and stability and soil resistance to penetration. Chemical soil quality was evaluated based on the assessment of the soil pH, C/N ratio and soil organic C content. At the long-term experiment an in depth focus on plant available P was carried out, using the DGT method, that mimics diffusion processes at plant root level. Yield and profitability were determined. Zero tillage plots with crop residue retention resulted in the best infiltration. Aggregate distribution and stability did mostly improve under zero tillage with residue retention, although no positive effects of this treatment were observed at the two driest locations. Tillage with residue removal resulted in the easiest penetrable topsoil, while zero tillage with residue retention created in most platforms a topsoil with a higher resistance to penetration. At deeper layers (30-60 cm) this trend was reversed. Most significant treatment effects on the soil chemical characteristics were observed in the topsoil (0-5 cm) where both the C/N ratio and soil organic C content tended to increase under zero tillage with crop residue retention. A significant treatment effect on plant available P was observed while conclusions on the most P beneficial treatment are yet to be drawn. No treatment effect on yield and profitability was observed. The overall results indicate a net positive effect of conservation agriculture on the sustainability levels of the assessed parameters. However, not all benefits of conservation agriculture can be generalized across all assessed locations. This emphasizes the importance of continued field research on how best to adapt the general principles to local circumstances in order to increase adoption of conservation agriculture.

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Dissertation
Agro-ecological niche of Bacterial Wilt in enset (Ensete ventricosum (Welw.) Cheesman), the 'tree against hunger'
Authors: --- --- ---
Year: 2017 Publisher: Leuven KU Leuven. Faculteit Bio-ingenieurswetenschappen

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Enset is an indigenous multipurpose plant on which more than 15 million people from south-western Ethiopia rely for subsistence. Nicknamed the ‘tree against hunger’, enset is a key species in assuring food security in the Ethiopian highlands. However, the enset-based farming system is severely threatened by Enset Xanthomonas Wilt (EXW), a disease caused by the bacterium Xanthomonas campestris pv. musacearum (Xcm). So far, most research has focused on the ways of transmission and sanitary control measures. Less is known about the disease’s relationship with ecological and management factors. This thesis research, designed in the framework of a VLIR-UOS project, aimed at investigating this relationship focusing on the Chencha catchment, near Arba Minch. Data were collected through farm surveys and soil and leaf sampling. Soil analyses revealed that enset homegardens are real fertility hotspots. Soil fertility decreased significantly with distance from the farmer’s house, with most soil factors (pH, TOC, TN, P, Ca, Mg and K) being largest in the zone nearest to the house (NR), followed by the outer zone (OR) of the homegarden. The nutrient content of the outfield (OF) was small. However, leaf analyses showed no significant differences in leaf nutrient contents of plants collected in the NR and OR zone, except for foliar nitrogen (N). Healthy and infected leaves differed significantly in potassium (K) and copper (Cu). Difficulties in comparing leaf nutrient content with values from literature highlights the need for creating or adopting a fixed leaf sampling strategy for enset and for defining optimal leaf nutrient levels. In the Chencha catchment, 20.2 % of the visited farms (n=256) were affected by the disease and EXW prevalence was found larger at lower elevations. A logistic regression model was built to predict EXW presence in a farm based on agro-ecological factors. Univariate logistic analysis indicated that larger soil P, Ca and Mg levels, a higher pH and intercropping increase the risk of EXW. Multiple logistic regression indicated that pH and intercropping had a significant positive effect on EXW presence, whereas the number of clones had a significant negative effect. However, it is not clear whether intercropping and the number of clones are the cause or rather a consequence of the disease. The fact that EXW was generally linked with farms having a large P and Ca content and a high pH, in addition to the low nutrient content of the outfields, might be an indication that farmers need to reconsider the spread of their fertilizer inputs. Moreover, further research should investigate the role of leafhoppers and nematodes in EXW transmission. To better understand the agro-ecological niche of EXW, it is also necessary to increase the sample size and to clarify certain questions in the questionnaire.

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Dissertation
The effect of combining organic inputs and N fertilizer on deep carbon sequestration: four long-term maize trials in Kenya

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Land use change is one of the major contributors to climate change. It is mainly caused by deforestation to expand the agricultural area. Since the attempts to cause a green revolution failed in sub-Saharan Africa (SSA), the large depletion in soil organic carbon (SOC) and nutrients has aggravated. This affects the yield of small-scale farmers. An approach for the agriculture in SSA to mitigate and adapt to climate change is the combined application of organic (OR) and mineral nitrogen (MR) inputs, which is a major principle of integrated soil fertility management. The combined application (OM) is hypothesized to have the largest potential to improve soil carbon sequestration. To verify this, the OR, MR and OM treatments are investigated in four long-term maize trials in Kenya. The MR is applied at a rate of 120 kg ha-1. The five OR quality classes are Tithonia diversifolia, Calliandra calothyrsus, maize stover, sawdust and farmyard manure. The classes have different amounts of polyphenol, lignin and nitrogen, which are the quality parameters of the OR. These classes are applied at a rate of 1.2 tons C ha-1 or 4 tons C ha-1. Therefore, the effect of both OR quality and OR quantity can be evaluated. The SOC stock is measured at a depth of 15 cm, 30 cm, 60 cm and 90 cm. Hence, the effect of the treatments in the subsoil can be analyzed. Additionally, the influence of clay and amorphous iron- (Feox) and aluminum- (Alox) oxyhydroxides to the SOC stock was investigated. These soil characteristics are expected to enhance the chemical stabilization of SOC resulting in better retention and, consequently, a larger SOC stock. A linear mixed effect model has been performed to evaluate the effect of treatments and soil characteristics to SOC stocks. Neither the treatment, nor the OR quality or the OR quantity turned out to be significantly contributing to increase SOC. Clay, Alox and Feox contents were significantly contributing to increase SOC due to chemical stabilization. The soils of Embu and Sidada contained a large amount of clay, Alox and Feox which resulted in a large SOC stock. The soils in Machanga and Aludeka had small amounts of clay, Alox and Feox and, consequently, small amounts of SOC.

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Dissertation
Interactions between phosphorus and manganese in crops grown on phosphate-fixing soils

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Soils of the tropics are often considered poor and contain low concentrations of phosphorus which is necessary for good plant growth. However, these soils contain very high concentrations of manganese. This can be toxic and reduce the growth of the plants. For an optimal plant growth, the soil should provide the plants with enough phosphorus but not too much manganese. In a previous research conducted in Kenya, soybean plants showed visual symptoms of manganese toxicity. These include a brown spots on the leaves and yellow colouring of the leaves. However, plants provided with phosphorus fertilisers were larger and did not present these symptoms. This posed the question if phosphorus fertilisation can directly reduce manganese toxicity in plants. This work aimed at investigating this possible interaction.Soybean plants were first grown in hydroculture at increasing magnanese concentrations at optimum to toxic levels combined with phosphorus supplied at deficient to optimum levels. Data was taken from the content of the plants and the content of the solution they were grown in. We found that plants with a Mn concentration of 660 mg/kg weigh only halve as much as plants grown under normal manganese conditions. Plants that did not receive enough phosphorus were found to be less sensitive to manganese toxicity and could tolerate higher concentrations of manganese. Another experiment was done with Kenyan soils where additional doses of manganese were added to increase the manganese concentrations in the soil. The results from this experiment confirmed that this soil was low in phosphorus and the toxic concentrations of manganese in the plant were in the same range as in the hydroculture.A last pot experiment was done on the soils where plants were with manganese toxicity symptoms to find if this was the cause for the low growth of the plants. The plants responded dramatically to phosphorus fertiliser, growth almost doubled. From the analysis of the content of the plan...

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Dissertation
Characterization of banana-based farming systems in the Upper Pangani Basin of Tanzania

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The highlands of the Upper Pangani Basin in Tanzania support a dense population of smallholder farmers. However, agriculture in the region is under increasing pressure due to population increase and climate change. In the future farmers must produce more food on less land under a changing and uncertain climate. Before advice can be given to farmers on how to cope, the current farming systems and their relation to the biotic and abiotic environment must first be understood. The overall objective of this thesis is to characterize the banana-based farming systems present on the southern slope of Mt. Kilimanjaro related to the different soil types present in the region. Three banana-based farming systems are characterized on the Southern slope of Mt. Kilimanjaro. In zone Up (1700 m) fields consist of a wide variety of crops and trees, and mainly food crops are grown. Thanks to abundant rainfall and fertile soils, households produce enough food to be self-sufficient year-round. The most important banana group is Mchare, which is cooked and consumed locally. In this elevation zone, an Umbric Nitisol with high organic carbon (TOC) is typical. Due to the high precipitation and weathering, generally a very low cation exchange capacity (CEC) and base saturation (BS) are found in the zone, which can be restricting for crop growth. The farming system in zone Mid (1300 m) is similar to zone Up, with a wide variety of crops grown in one field. However, rainfall is more seasonal, and dry seasons are more pronounced. As a result the majority of the households struggle to produce enough food for a few months per year, and must buy food. Banana production is 10 times higher than in zone Up and the Mchare and Cavendish form the two most important groups. Besides being a food crop, banana is also a cash crop along with coffee. At around 1400 m, a Silandic Andosol is analyzed, with low CEC and moderate BS and TOC. A major problem for the soils in Up and Mid is phosphorus (P) fixation, evident from low P content and deficiency in maize. At around 1000 m is an Ortho-eutric Mollic Nitisol. As elevation decreases, TOC decreases to low while BS increases to high, and CEC and P are moderate. Due to population pressure in zone Up and Mid people settled in the lowlands at the foot of Mt. Kilimanjaro, or zone Low (750m). Rainfall is scarce, so irrigation is needed to grow crops. By locating close to permanent water sources, food can be produced year-round, making farmers self-sufficient. Crops are grown in monoculture, and the major banana is Cavendish, produced for export. In this zone we find a Luvic Phaeozem. With less precipitation and higher temperature, TOC decreases to Low. High CEC and BS are typical of this generally fertile soil, where farming has not been present as long as in Mid and Up. This soil is suitable for irrigated agriculture and raising cattle, which are two major activities in the region.

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Dissertation
The potential use of clinoptilolite in controlled-release fertilizer production

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Nitrogen, also the amounts applied by fertilizers, can leach or volatilize out of the soil, causing both ecological problems and economical losses. Therefore, the synchronization between nitrogen supply and the demand of the crop has been a long-standing management goal. Controlled-release fertilizers (CRFs) could serve as a solution. A potential, natural material as matrix or coating for the CRFs is clinoptilolite, a zeolite. Clinoptilolite has a low cost and high affinity for the ammonium cation, which makes it an interesting material for agriculture. In the first part of this research, the cations naturally present on the clinoptilolite were exchanged with ammonium and a few characteristics of the exchanged and non-exchanged clinoptilolite were determined. The second part consists of an incubation experiment, where nitrate formation was measured over time. From this, the potential nitrification rate (PNR) was determined and did not differ between treatments with ammonium-loaded clinoptilolite, NPK fertilizer and NPK with clinoptilolite added. Thus, clinoptilolite has no effect on the nitrification rate in an incubation experiment. The proton production rate was also calculated and was higher for the treatment with ammonium-loaded clinoptilolite. This result does not correlate with what was found for the nitrate concentration over time, which may indicate that the proton production is not a good estimate for the nitrification. In the final part of this research a plant growth experiment was conducted. Here, clinoptilolite had a positive effect on the length and aboveground biomass of the plants in a sandy soil. Evapotranspiration water losses were also recorded during the growth period, to estimate the growth of the plants. In the sandy soil, clinoptilolite had a positive effect on the growth rate of the plants. For the loamy soil, patterns were not clear. In general clinoptilolite had a positive effect compared to the control, but this effect is overruled when fertilizer is added.

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Dissertation
Carbon cycling from a catchment perspective : an integrated approach to terrestrial-aquatic linkages in the Tana River basin, Kenya.
Authors: --- --- --- ---
ISBN: 9789088263125 Year: 2013 Publisher: Leuven KU Leuven. Faculteit Bio-ingenieurswetenschappen

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Abstract

Rivers play an important role in the global carbon (C) cycle. They do not merely transport C from the terrestrial biome to the oceanic environment, but also bury and process organic matter, and thereby generally act as a source of CO2 to the atmosphere. This study focuses on riverine C cycling in Tana River Basin, Kenya, the longest river in Kenya (~1100 km), with a total catchment area of ~96,000 km². Data were collected during three basin-wide surveys along an altitudinal gradient, from 3600 m in headwaters to 8 m in lower Tana River in February 2008 (dry season), September-November 2009 (wet season), and June-July 2010 (end of wet season) as well as monthly sampling at three sites in lower Tana River between January 2009 and December 2011. A consistent downstream increase in total suspended matter (TSM, 0.6 to 7058 mg L-1) and particulate organic carbon (POC) was observed during all three sampling campaigns. With the exception of reservoir waters, POC was predominantly of terrestrial origin as indicated by generally high POC: Chlorophyll a (POC: Chl-a) ratios. Stable isotope signatures of POC (d13CPOC) ranged between -32‰ and -20‰ and increased downstream, reflecting an increasing contribution of C4-derived carbon. Dissolved organic carbon (DOC) concentrations were highest during the end of wet season (2.1 to 6.9 mg L-1), with stable isotope signatures generally between -28‰ and -22‰. A consistent downstream decrease in % organic carbon (%OC) was observed for soils, riverine sediments, and suspended matter. This was likely due to better preservation of the organic fraction in colder high altitude regions, with loss of carbon during downstream spiraling. d13C values for soil and sediment did not exhibit clear altitudinal patterns, but values reflect the full spectrum from C3-dominated to C4-dominated sites. With few exceptions, the entire riverine network was supersaturated in CO2. pCO2 values were generally higher in the lower main Tana River compared to headwater tributaries, opposite to the pattern typically observed in other river networks. First-order estimates show in-stream community respiration was responsible for the bulk of total CO2 evasion (59% to 89%) in the main Tana River while in tributaries respiration accounted for 4% to 52% of total CO2 evasion, suggesting CO2 evasion in tributaries was sustained by lateral CO2 inputs. While sediment loads increase downstream, both chlorophyll a (0.2 µg L-1 to 9.6 µg L-1) and primary production (0.004 µmol L-1 h-1 to 7.38 µmol L-1 h-1) increased consistently downstream. Diurnal fluctuations of biogeochemical parameters were found to be substantial only in the headwater stream, moderate in the reservoir and not detectable at main Tana River. The C sources supporting heterotrophic bacteria in both terrestrial soils and riverine sediments (bottom and suspended) were examined using d13C signatures of bacterial phospholipid-derived fatty acids (PLFA). Generally, bacterial communities appeared to use C3 and C4-derived carbon in the same proportions as they occur in the bulk organic matter pool across different environments. However, a positive correlation between d13C signatures of bacterial and green algal PLFA in the tributaries provides an indication for preferential use of this more labile C source when available. Annual fluxes of suspended sediments and different carbon species were quantified at different stations in the lower Tana River. Both TSM (24 to 9386 mg L-1) and POC (0.8 to 141.9 mg C L-1) concentrations were highly variable. DOC and DIC concentrations ranged from 0.8 to 5.2 mg C L-1 and 7.1 to 23.5 mg C L-1, respectively, but their seasonal variations were not systematic. A strong shift in the origin of POC was observed, being dominated by C3-derived C during dry conditions (low d13CPOC signatures between -28‰ and -25‰), but with significant C4 contributions during higher flow events (d13CPOC up to -19.5 ‰). In contrast, d13CDOC did not show any clear pattern with discharge despite the high range (-27.2‰ to -17.4‰). Over 80% of the sediment and organic carbon was transported during the high flow seasons (March to May and October to December combined). The floodplains along the main Tana River appear to play a critical role in regulating the transport of sediments and organic carbon as reflected by a significant reduction (35-65%) in the sediment, POC and DOC delivery along the stretch between Garissa and Tana River Primate Reserve (~350 km). Considering the additional trapping of sediments and carbon in the upstream reservoirs, and in floodplains downstream of the studied river section, it becomes evident that a major fraction of the sediment and C inputs into the Tana River network are retained and/or processed prior to entering the Indian Ocean.

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