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Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout Flow Cytometry: Principles and Applications, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.

Flow cytometry. --- Cell separation. --- Cell isolation --- Cell segregation --- Cells --- Cytometry --- Separation (Technology) --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Separation --- Cell separation --- Cytodiagnosis --- Flow cytometry --- Cell Separation --- Flow Cytometry --- Cytologic diagnosis --- Diagnosis, Cytologic --- Diagnostic cytopathology --- Diagnosis, Laboratory --- Pathology, Cellular --- methods

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Histology. Cytology --- Semiology. Diagnosis. Symptomatology --- Human medicine --- klinische laboratoria --- medische laboratoriumtechnologie --- cytologie --- histologie

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Flow cytometry and laser scanning cytometry are increasingly used in clinical and research settings, following improvements in instrument design and computing power and the increased availability of fluorescent agents. This book provides a comprehensive introduction to the theory and clinical applications of these techniques in the assessment of cell phenotype and function. With an emphasis on clinical relevance, the book presents the principles and potential of cytometry in the analysis of phenomena including cell-mediated cytotoxicity, metabolic burst, phagocytosis, cell-cell aggregation, receptor shedding and apoptosis. Guidance is given on data interpretation, quality control procedures, pitfalls and problems. All chapters contain detailed protocols from leading authorities in the field with extensive practical experience.

Flow cytometry --- Cytology