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This volume encompasses all major methodologies to interrogate endocannabinoid systems (ECS) and endocannabinoids (eCBs) signaling. With increasing interest towards the manifold activities of eCBs, this book discusses the chemical, biochemical, and molecular biological assays, and activity of distinct elements of the ECS. These include membrane, nuclear receptors, biosynthetic and hydrolytic enzymes, and membrane transporters and oxidative enzymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocols is a valuable resource and will help chemists, drug designers, biochemists, molecular biologists, cell biologists, pharmacologists, and (electro) physiologists navigate the mare magnum of endocannabinoid research.

Neurosciences. --- Neural sciences --- Neurological sciences --- Neuroscience --- Medical sciences --- Nervous system

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During the last 60 years the relevance of cannabis (Cannabis sativa or Cannabis indica) ingredients, like the psychoactive Δ9-tetrahydrocannabinol (THC), cannabidiol, 120+ additional cannabinoids and 440+ non-cannabinoid compounds, for human health and disease has become apparent. Approximately 30 years after the elucidation of THC structure the molecular reasons for the biological activity of these plant extracts were made clearer by the discovery of endocannabinoids, that are endogenous lipids able to bind to the same receptors activated by THC. Besides endocannabinoids, that include several N-acylethanolamines and acylesters, a complex array of receptors, metabolic enzymes, transporters (transmembrane, intracellular and extracellular carriers) were also discovered, and altogether they form a so-called “endocannabinoid system” that has been shown to finely tune the manifold biological activities of these lipid signals. Both plant-derived cannabinoids and endocannabinoids were first discovered by the group led by Prof. Dr. Raphael Mechoulam, who has just celebrated his 90th birthday and clearly stood out as a giant of modern science. The many implications of his seminal work for chemistry, biochemistry, biology, pharmacology and medicine are described in this special issue by the scientists who reached during the last 20 years the highest recognition in the field of (endo)cannabinoid research, receiving the Mechoulam Award for their major contributions. I thank them for having accepted my invitation to be part of this honorary issue of Molecules, and Raphi for continuing to illuminate our field with his always inspiring investigations and new ideas.

cannabinoid --- MRI-1867 --- hybrid ligand --- CB1 receptor antagonist --- iNOS inhibitor --- rimonabant --- intracerebroventricular administration --- alcohol craving --- two-bottle paradigm --- drinking in the dark --- N-acyltransferase --- anandamide --- endocannabinoid --- phospholipase A2 --- cannabichromene --- cannabidiolic acid --- cannabidivarin --- cannabidivarinic acid --- phytocannabinoids --- tetrahydrocannabivarin --- 4′-fluoro-cannabidiol --- cannabinoid tetrad --- elevated plus maze --- catalepsy --- marble bury --- HUF-101 --- equilibrative nucleoside transporter --- CB1 --- biased signaling --- functional selectivity --- G-protein --- β-arrestin --- cannabigerol --- anti-inflammatory --- obesity --- cannabinoid receptor 2 (CB2R) --- microglia --- inflammaging --- memory --- lipofuscin --- aminoalkylindole --- allodynia --- antinociception --- cannabinoid receptor --- CP55940 --- JWH-018 --- K2 --- pravadoline --- spice --- WIN55212-2 --- type 1 cannabinoid receptor CB1 --- cholesterol --- hippocampus --- frontal cortex --- synaptosomes --- rescue model --- anti-CB1 antibody --- cannabinoids --- GPR55 receptors --- VCE-006.1 --- chromenopyrazole --- Parkinson’s disease --- 6-hydroxydopamine --- lipopolysaccharide --- amyotrophic lateral sclerosis --- mSOD1 mice --- TDP-43 transgenic mice --- PPARs --- gut microbiome --- intestine --- ghrelin --- LEAP2 --- n/a --- 4'-fluoro-cannabidiol --- Parkinson's disease

Choose an application

• Reference Manager
• EndNote
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During the last 60 years the relevance of cannabis (Cannabis sativa or Cannabis indica) ingredients, like the psychoactive Δ9-tetrahydrocannabinol (THC), cannabidiol, 120+ additional cannabinoids and 440+ non-cannabinoid compounds, for human health and disease has become apparent. Approximately 30 years after the elucidation of THC structure the molecular reasons for the biological activity of these plant extracts were made clearer by the discovery of endocannabinoids, that are endogenous lipids able to bind to the same receptors activated by THC. Besides endocannabinoids, that include several N-acylethanolamines and acylesters, a complex array of receptors, metabolic enzymes, transporters (transmembrane, intracellular and extracellular carriers) were also discovered, and altogether they form a so-called “endocannabinoid system” that has been shown to finely tune the manifold biological activities of these lipid signals. Both plant-derived cannabinoids and endocannabinoids were first discovered by the group led by Prof. Dr. Raphael Mechoulam, who has just celebrated his 90th birthday and clearly stood out as a giant of modern science. The many implications of his seminal work for chemistry, biochemistry, biology, pharmacology and medicine are described in this special issue by the scientists who reached during the last 20 years the highest recognition in the field of (endo)cannabinoid research, receiving the Mechoulam Award for their major contributions. I thank them for having accepted my invitation to be part of this honorary issue of Molecules, and Raphi for continuing to illuminate our field with his always inspiring investigations and new ideas.

Research & information: general --- Biology, life sciences --- Biochemistry --- cannabinoid --- MRI-1867 --- hybrid ligand --- CB1 receptor antagonist --- iNOS inhibitor --- rimonabant --- intracerebroventricular administration --- alcohol craving --- two-bottle paradigm --- drinking in the dark --- N-acyltransferase --- anandamide --- endocannabinoid --- phospholipase A2 --- cannabichromene --- cannabidiolic acid --- cannabidivarin --- cannabidivarinic acid --- phytocannabinoids --- tetrahydrocannabivarin --- 4'-fluoro-cannabidiol --- cannabinoid tetrad --- elevated plus maze --- catalepsy --- marble bury --- HUF-101 --- equilibrative nucleoside transporter --- CB1 --- biased signaling --- functional selectivity --- G-protein --- β-arrestin --- cannabigerol --- anti-inflammatory --- obesity --- cannabinoid receptor 2 (CB2R) --- microglia --- inflammaging --- memory --- lipofuscin --- aminoalkylindole --- allodynia --- antinociception --- cannabinoid receptor --- CP55940 --- JWH-018 --- K2 --- pravadoline --- spice --- WIN55212-2 --- type 1 cannabinoid receptor CB1 --- cholesterol --- hippocampus --- frontal cortex --- synaptosomes --- rescue model --- anti-CB1 antibody --- cannabinoids --- GPR55 receptors --- VCE-006.1 --- chromenopyrazole --- Parkinson's disease --- 6-hydroxydopamine --- lipopolysaccharide --- amyotrophic lateral sclerosis --- mSOD1 mice --- TDP-43 transgenic mice --- PPARs --- gut microbiome --- intestine --- ghrelin --- LEAP2 --- cannabinoid --- MRI-1867 --- hybrid ligand --- CB1 receptor antagonist --- iNOS inhibitor --- rimonabant --- intracerebroventricular administration --- alcohol craving --- two-bottle paradigm --- drinking in the dark --- N-acyltransferase --- anandamide --- endocannabinoid --- phospholipase A2 --- cannabichromene --- cannabidiolic acid --- cannabidivarin --- cannabidivarinic acid --- phytocannabinoids --- tetrahydrocannabivarin --- 4'-fluoro-cannabidiol --- cannabinoid tetrad --- elevated plus maze --- catalepsy --- marble bury --- HUF-101 --- equilibrative nucleoside transporter --- CB1 --- biased signaling --- functional selectivity --- G-protein --- β-arrestin --- cannabigerol --- anti-inflammatory --- obesity --- cannabinoid receptor 2 (CB2R) --- microglia --- inflammaging --- memory --- lipofuscin --- aminoalkylindole --- allodynia --- antinociception --- cannabinoid receptor --- CP55940 --- JWH-018 --- K2 --- pravadoline --- spice --- WIN55212-2 --- type 1 cannabinoid receptor CB1 --- cholesterol --- hippocampus --- frontal cortex --- synaptosomes --- rescue model --- anti-CB1 antibody --- cannabinoids --- GPR55 receptors --- VCE-006.1 --- chromenopyrazole --- Parkinson's disease --- 6-hydroxydopamine --- lipopolysaccharide --- amyotrophic lateral sclerosis --- mSOD1 mice --- TDP-43 transgenic mice --- PPARs --- gut microbiome --- intestine --- ghrelin --- LEAP2