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Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time-consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.
Analytical biochemistry --- Gene expression --- Polymerase chain reaction --- Reverse transcriptase --- Research --- Methodology --- Biochemical Processes --- Nucleic Acid Amplification Techniques --- Gene Expression --- Chemical Processes --- Genetic Techniques --- Biochemical Phenomena --- Genetic Processes --- Chemical Phenomena --- Investigative Techniques --- Genetic Phenomena --- Phenomena and Processes --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Polymerase Chain Reaction --- Transcription, Genetic --- Biology --- Health & Biological Sciences --- Genetics --- Cell biology. --- Biochemistry. --- Cell Biology. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Cell biology --- Cellular biology --- Cells --- Composition --- Gene expression - Research - Methodology
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This Special Issue of Marine Drugs gathers recent investigations on the proteomes, metabolomes, transcriptomes, and the associated microbiomes of marine jellyfish and polyps, including bioactivity studies of their compounds and more generally, on their biotechnological potential, witnessing the increasingly recognized importance of Cnidaria as a largely untapped Blue Growth resource for new drug discovery. These researches evoke the outstanding ecological importance of cnidarians in marine ecosystems worldwide, calling for a global monitoring and conservation of marine biodiversity, so that the biotechnological exploitation of marine living resources will be carried out to conserve and sustainably use the natural capital of the oceans.
Technology: general issues --- cnidarian --- sea anemone --- proteins --- toxins --- two-dimensional gel electrophoresis --- MALDI-TOF/TOF --- shotgun proteomic --- Zoanthidea --- holo-transcriptome --- cnidarian transcriptome --- marine enzyme --- marine biocatalyst --- marine biotechnology --- pharmaceutical biotechnology --- anthozoa --- microbial communities --- cnidarian holobiont --- zooxanthellae --- bleaching --- antibacterial activity --- jellyfish --- Aurelia coerulea --- mucus --- proteomics --- metabolomics --- cnidarians --- gelatinous zooplankton --- bioprospecting --- novel foods --- transcriptomics --- bio-prospecting --- computational biology --- neurotoxins --- NMR spectroscopy --- biochemical characterization --- jellyfish blooms --- Cnidaria --- Ctenophora --- biodiversity --- bioactive compounds --- blue biotechnology --- invertebrate proteins --- biological activity --- antioxidants --- collagen --- pepsin hydrolysis --- collagenase hydrolysis --- oxidative stress --- keratinocytes --- cytotoxicity --- Easter Island --- Actinobacteria --- anthraquinones --- symbionts --- marine invertebrates --- spectroscopy --- chromatography --- Pelagia noctiluca --- Mediterranean jellyfish --- chemical characterization --- aquafeed and food supplements --- sustainable fishing
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This Special Issue of Marine Drugs gathers recent investigations on the proteomes, metabolomes, transcriptomes, and the associated microbiomes of marine jellyfish and polyps, including bioactivity studies of their compounds and more generally, on their biotechnological potential, witnessing the increasingly recognized importance of Cnidaria as a largely untapped Blue Growth resource for new drug discovery. These researches evoke the outstanding ecological importance of cnidarians in marine ecosystems worldwide, calling for a global monitoring and conservation of marine biodiversity, so that the biotechnological exploitation of marine living resources will be carried out to conserve and sustainably use the natural capital of the oceans.
cnidarian --- sea anemone --- proteins --- toxins --- two-dimensional gel electrophoresis --- MALDI-TOF/TOF --- shotgun proteomic --- Zoanthidea --- holo-transcriptome --- cnidarian transcriptome --- marine enzyme --- marine biocatalyst --- marine biotechnology --- pharmaceutical biotechnology --- anthozoa --- microbial communities --- cnidarian holobiont --- zooxanthellae --- bleaching --- antibacterial activity --- jellyfish --- Aurelia coerulea --- mucus --- proteomics --- metabolomics --- cnidarians --- gelatinous zooplankton --- bioprospecting --- novel foods --- transcriptomics --- bio-prospecting --- computational biology --- neurotoxins --- NMR spectroscopy --- biochemical characterization --- jellyfish blooms --- Cnidaria --- Ctenophora --- biodiversity --- bioactive compounds --- blue biotechnology --- invertebrate proteins --- biological activity --- antioxidants --- collagen --- pepsin hydrolysis --- collagenase hydrolysis --- oxidative stress --- keratinocytes --- cytotoxicity --- Easter Island --- Actinobacteria --- anthraquinones --- symbionts --- marine invertebrates --- spectroscopy --- chromatography --- Pelagia noctiluca --- Mediterranean jellyfish --- chemical characterization --- aquafeed and food supplements --- sustainable fishing
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This Special Issue of Marine Drugs gathers recent investigations on the proteomes, metabolomes, transcriptomes, and the associated microbiomes of marine jellyfish and polyps, including bioactivity studies of their compounds and more generally, on their biotechnological potential, witnessing the increasingly recognized importance of Cnidaria as a largely untapped Blue Growth resource for new drug discovery. These researches evoke the outstanding ecological importance of cnidarians in marine ecosystems worldwide, calling for a global monitoring and conservation of marine biodiversity, so that the biotechnological exploitation of marine living resources will be carried out to conserve and sustainably use the natural capital of the oceans.
Technology: general issues --- cnidarian --- sea anemone --- proteins --- toxins --- two-dimensional gel electrophoresis --- MALDI-TOF/TOF --- shotgun proteomic --- Zoanthidea --- holo-transcriptome --- cnidarian transcriptome --- marine enzyme --- marine biocatalyst --- marine biotechnology --- pharmaceutical biotechnology --- anthozoa --- microbial communities --- cnidarian holobiont --- zooxanthellae --- bleaching --- antibacterial activity --- jellyfish --- Aurelia coerulea --- mucus --- proteomics --- metabolomics --- cnidarians --- gelatinous zooplankton --- bioprospecting --- novel foods --- transcriptomics --- bio-prospecting --- computational biology --- neurotoxins --- NMR spectroscopy --- biochemical characterization --- jellyfish blooms --- Cnidaria --- Ctenophora --- biodiversity --- bioactive compounds --- blue biotechnology --- invertebrate proteins --- biological activity --- antioxidants --- collagen --- pepsin hydrolysis --- collagenase hydrolysis --- oxidative stress --- keratinocytes --- cytotoxicity --- Easter Island --- Actinobacteria --- anthraquinones --- symbionts --- marine invertebrates --- spectroscopy --- chromatography --- Pelagia noctiluca --- Mediterranean jellyfish --- chemical characterization --- aquafeed and food supplements --- sustainable fishing --- cnidarian --- sea anemone --- proteins --- toxins --- two-dimensional gel electrophoresis --- MALDI-TOF/TOF --- shotgun proteomic --- Zoanthidea --- holo-transcriptome --- cnidarian transcriptome --- marine enzyme --- marine biocatalyst --- marine biotechnology --- pharmaceutical biotechnology --- anthozoa --- microbial communities --- cnidarian holobiont --- zooxanthellae --- bleaching --- antibacterial activity --- jellyfish --- Aurelia coerulea --- mucus --- proteomics --- metabolomics --- cnidarians --- gelatinous zooplankton --- bioprospecting --- novel foods --- transcriptomics --- bio-prospecting --- computational biology --- neurotoxins --- NMR spectroscopy --- biochemical characterization --- jellyfish blooms --- Cnidaria --- Ctenophora --- biodiversity --- bioactive compounds --- blue biotechnology --- invertebrate proteins --- biological activity --- antioxidants --- collagen --- pepsin hydrolysis --- collagenase hydrolysis --- oxidative stress --- keratinocytes --- cytotoxicity --- Easter Island --- Actinobacteria --- anthraquinones --- symbionts --- marine invertebrates --- spectroscopy --- chromatography --- Pelagia noctiluca --- Mediterranean jellyfish --- chemical characterization --- aquafeed and food supplements --- sustainable fishing
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Plant ecology. Plant sociology --- Plant physiology. Plant biophysics --- Crops --- Plants --- Plant gene expression --- Effect of stress on --- Congresses --- Plants [Effect of stress on ] --- Plant gene expression - Congresses. --- Crops - Effect of stress on - Congresses. --- Plants - Effect of stress on - Congresses. --- Plants, Effect of stress on. Congresses --- Crops. Effect of stress on. Congresses --- Plant gene expression. Congresses
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