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Despite major advances in the treatment of HIV, there are still many areas requiring additional study. These areas include ways to create efficacious, potent antiretrovirals for suppressing viral loads completely without toxic side effects, the definition of unknown drug targets while fine-tuning known targets, and the ability to better understand the interplay between viral and host factors. In HIV Protocols, Second Edition, expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, HIV Protocols, Second Edition is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.

Emerging infectious diseases. --- Immunology. --- Medical virology. --- Infectious Diseases. --- Virology. --- Immunobiology --- Life sciences --- Serology --- Medical microbiology --- Virology --- Virus diseases --- Emerging infections --- New infectious diseases --- Re-emerging infectious diseases --- Reemerging infectious diseases --- Communicable diseases