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Gregorio Weber is widely acknowledged as the person responsible for the advent of modern fluorescence spectroscopy.Since 2016 is the 100th anniversary of Gregorio Weber’s birth, this special volume has been prepared to honor his life and achievements. It offers contributions from outstanding researchers in the fluorescence field, describing their perspectives on modern fluorescence and its highly diverse applications, ranging from the photophysics of tryptophan and proteins, membrane studies, fluorescence microscopy on live cells, novel software approaches and instrumentation. Many of the authors knew Gregorio Weber personally and have shared their impressions of the man and his contributions. This volume appeals not only to aficionados of fluorescence spectroscopy and its applications in biology, chemistry and physics, but also to those with a general interest in the historical development of an important scientific field.

Chemistry. --- Molecular biology. --- Spectroscopy. --- Physical chemistry. --- Chemistry --- Microscopy. --- Spectroscopy/Spectrometry. --- Spectroscopy and Microscopy. --- Molecular Medicine. --- Physical Chemistry. --- History of Chemistry. --- History. --- Fluorescence spectroscopy. --- Fluorescence spectrometry --- Spectrometry, Fluorescence --- Spectroscopy, Fluorescence --- Luminescence spectroscopy --- Fluorescent probes --- Medicine. --- Chemistry, Physical organic. --- Chemistry-History. --- Chemistry, Physical organic --- Chemistry, Organic --- Chemistry, Physical and theoretical --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Analysis, Spectrum --- Spectra --- Spectrochemical analysis --- Spectrochemistry --- Spectroscopy --- Chemistry, Analytic --- Interferometry --- Optics --- Radiation --- Wave-motion, Theory of --- Absorption spectra --- Light --- Spectroscope --- Qualitative --- Health Workforce --- Chemistry—History. --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Spectrometry --- Analytical chemistry

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"A concise introduction to fundamental principles, this book presents the most important aspects of fluorescence spectroscopy, particularly as they are applied to biology, biochemistry, and biophysics. The author focuses on the essential aspects of fluorescence to encourage readers to design experiments, begin their own fluorescence measurements, and access the literature with greater comprehension. After a general definition of the field and an overview of the nature of light and absorption by biomolecules, the text covers instrumentation, emission spectra, excitation spectra, polarization, anisotropy, quenching, time-resolved methods, and FRET"--

Biomolecules --- Fluorescence spectroscopy --- Analysis