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Focused on one of the most important and fascinating fields of modern biology, Exocytosis and Endocytosis, Second Edition presents a comprehensive collection of protocols for cutting edge, recently developed methods of studying exocytosis and endocytosis in vitro and in vivo. Divided into four easy-to-follow sections, chapters focus on diverse research topics including cell-free and biochemical assays of exocytosis and endocytosis, probing protein, membrane, and organelle trafficking in cultured cells, and powerful in vivo imaging approaches to investigate different physiological processes involving exocytosis and endocytosis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Highly practical and clearly written, Exocytosis and Endocytosis, Second Edition will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining protein and membrane trafficking in simple cellular systems and model organisms.

Cytology. --- Cell physiology. --- Cell Biology. --- Cell Physiology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Cell function --- Cytology --- Physiology

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Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts provide the most up-to-date,step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. Following the highly successful Methods in Molecular Biology™ series format, the chapters present an introduction outlining the principle behind each technique, a list of the necessary materials, an easy to follow, readily reproducible protocol, and a Notes section offering tips on troubleshooting and avoiding known pitfalls. Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.

Cytology. --- Human physiology. --- Developmental biology. --- Immunology. --- Neurobiology. --- Cell Biology. --- Human Physiology. --- Developmental Biology. --- Immunobiology --- Life sciences --- Serology --- Development (Biology) --- Biology --- Growth --- Ontogeny --- Human biology --- Medical sciences --- Physiology --- Human body --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Neurosciences