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The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research in the 21st century. In Apoptosis Methods and Protocols, highly experienced researchers in the field of apoptosis describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process. These readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Apoptosis Methods and Protocols constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.
Apoptosis --- Molecular biology --- DNA Damage --- physiology --- Apoptosis -- Laboratory manuals. --- Molecular biology -- Laboratory manuals. --- Biological Science Disciplines --- Genetic Processes --- Cell Death --- Publication Formats --- Publication Characteristics --- Genetic Phenomena --- Cell Physiological Processes --- Natural Science Disciplines --- Disciplines and Occupations --- Phenomena and Processes --- Cell Physiological Phenomena --- Laboratory Manuals --- Physiology --- Biology --- Health & Biological Sciences --- Cytology --- Cell death --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Apoptosis - Laboratory manuals. --- Molecular biology - Laboratory manuals. --- DNA Damage - physiology - Laboratory Manuals. --- Apoptosis - physiology - laboratory manuals
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A century has passed since the Komagata Maru arrived in Vancouver. Its arrival was a direct challenge to Canada's immigration laws, which barred immigrants from India – yet the nearly four hundred Punjabi passengers on board the ship had been promised equality with all other British subjects, and they arrived to claim that right. The Voyage of the Komagata Maru is an extensive revision, reappraisal, and expansion of Hugh Johnston's authoritative history of the Komagata Maru incident, first published in 1979. The updated edition draws in new research – exploring legal issues and the motives of the passengers and their leaders and supporters – and revisits the previous edition's assessments in light of insight gained over the intervening decades. Now expanded by more than 50 percent, this landmark book is still the only comprehensive historical account of the Komagata Maru incident – a story of immigration, empire, and politics, which Canadians increasingly recognize as a critical moment in this country's history.
Sikhs --- Aliens --- History. --- Enemy aliens --- Expatriates --- Foreign citizens (Aliens) --- Foreign population --- Foreign residents --- Foreigners --- Noncitizens --- Resident aliens --- Unnaturalized foreign residents --- Persons --- Deportees --- Exiles --- Immigrants --- Refugees --- Ethnology --- Religious adherents --- Legal status, laws, etc. --- Illegal aliens --- Illegal immigrants --- Non-citizens --- Unauthorized immigrants --- Undocumented aliens --- Undocumented immigrants --- Komagatamaru (Ship) --- Canada --- India --- Emigration and immigration. --- Politics and government --- Kāmāgāṭāmārū (Ship) --- Komagata Maru (Ship)
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British Columbia --- Colombie-Britannique --- History --- Histoire
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In Jewels of the Qila, Hugh Johnston draws on memoirs and interviews, newspaper articles and photographs, to tell the story of three generations of a remarkable Sikh family and the communities they lived in and supported in both Canada and India. The Siddoos are Punjabi. Kapoor Singh, father and grandfather, arrived in British Columbia in 1912 and had to overcome racial prejudice and legal discrimination to transform himself from labourer to lumber baron. As he campaigned for citizenship and immigration rights for his people, he and his wife, Besant Kaur, fostered in their daughters a vision of service and activism that, as adults, they fulfilled by establishing a family-run hospital in Punjab and by introducing a Westernized version of an Indian spiritual tradition to Canada. The Siddoos are the heart of the story, but their history tells a larger tale of an immigrant community's triumphs and tribulations and the strong connection that Indo-Canadians continue to forge with their homeland.
Sikhs --- Siddoo family. --- British Columbia --- History.
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Immigrants --- East Indians --- Indiens (Orientaux) --- History --- History. --- Histoire
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