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Here is a unique, practical volume that covers the full range of issues surrounding the expanding field of nucleic acid sequencing. It provides advice on the methods of Sanger, Maxam, and Gilbert, while paying close attention to such recent technological advances as the development of automated sequencing and the refinement of computer hardware and software. This unique interdisciplinary guide also includes information on how the new methodologies have affected RNA sequencing, and other areas as diverse as archaeology and forensic work. into RNA sequencing, and other areas as diverse as archaeology and forensic work.
Nucleotide sequence --- Base Sequence --- Computers --- DNA --- RNA --- Technique --- analysis --- Base Sequence. --- Computers. --- 577.212.3 --- #WSCH:WBIO --- Calculators, Programmable --- Computer Hardware --- Computers, Digital --- Hardware, Computer --- Calculator, Programmable --- Computer --- Computer, Digital --- Digital Computer --- Digital Computers --- Programmable Calculator --- Programmable Calculators --- DNA Sequences --- DNA Sequence --- Nucleotide Sequence --- RNA Sequence --- Base Sequences --- Nucleotide Sequences --- RNA Sequences --- Sequence, Base --- Sequence, DNA --- Sequence, Nucleotide --- Sequence, RNA --- Sequences, Base --- Sequences, DNA --- Sequences, Nucleotide --- Sequences, RNA --- Genes --- Nucleotides --- analysis. --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Technique. --- Analysis. --- 577.212.3 Nucleic acid base and sequence compositon. Experimental deciphering of genetic code. --- Base sequence. --- Dna --- Rna --- Analysis, Nucleic acid sequence --- Analysis, Nucleotide sequence --- Base sequence (Nucleic acids) --- DNA sequence --- Nucleic acid sequence analysis --- Nucleotide sequence analysis --- RNA sequence --- Nucleic acids --- Sequence alignment (Bioinformatics) --- Nucleic acid base and sequence compositon. Experimental deciphering of genetic code --- Analysis --- Nucleotide sequence - Technique. --- Nucleotide sequence - Technique --- DNA - analysis --- RNA - analysis
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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Molecular cloning --- 575.5 --- Genetische manipulatie --- Klonen --- Cloning, Molecular --- DNA cloning --- Gene cloning --- Cloning --- Genetic engineering --- Molecular genetics --- Clone cells --- Molecular cloning.
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