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Nucleic acids sequencing : a practical approach
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ISBN: 0199630569 0199630577 9780199630578 9780199630561 Year: 1989 Volume: vol *28 Publisher: Oxford: IRL press,

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Here is a unique, practical volume that covers the full range of issues surrounding the expanding field of nucleic acid sequencing. It provides advice on the methods of Sanger, Maxam, and Gilbert, while paying close attention to such recent technological advances as the development of automated sequencing and the refinement of computer hardware and software. This unique interdisciplinary guide also includes information on how the new methodologies have affected RNA sequencing, and other areas as diverse as archaeology and forensic work. into RNA sequencing, and other areas as diverse as archaeology and forensic work.

Gene cloning and manipulation
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ISBN: 9780511807343 9780521817936 9780521521055 9780511296536 0511296533 0511295766 9780511295768 0511807341 9780511294198 0511294190 0521817935 052152105X 1107174376 9781107174375 1280959681 9781280959684 9786610959686 6610959684 0511561873 9780511561870 0511294999 9780511294990 Year: 2007 Publisher: Cambridge, UK ; New York : Cambridge University Press,

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Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.


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Molecular aspects of light-harvesting processes in algae
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