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This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for the major procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements.
Gel electrophoresis. --- Proteins --- Analysis. --- GEL ELECTROPHORESIS --- PROTEINS --- ANALYSIS
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The regulation of eukaryotic gene expression is a fundamental and highly active area of current research. This work brings together leading workers in the field for the first time to review the latest findings on all major aspects of transcription and processing, and their regulation. Knowledge of these events is increasing rapidly, with great significance for all those working in molecular biology, making this volume a timely source of invaluable information.
Molecular biology --- RNA Splicing --- Genetic transcription --- RNA splicing --- Eukaryotic cells --- Transcription génétique --- Epissage --- Cellules eucaryotes --- Transcription, Genetic --- Eukaryotic cells. --- RNA splicing. --- RNA Splicing. --- Transcription, Genetic. --- Basic Sciences. Molecular Biology -- Nucleic Acids --- Basic Sciences Molecular Biology -- Nucleic Acids. --- Rna splicing. --- Transcription, genetic. --- Basic sciences molecular biology -- nucleic acids. --- Genetic transcription.
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Here is a newly updated and expanded edition of a useful and popular guide. In addition to providing new information on topics covered in the first edition, this volume covers a number of major techniques which must be mastered by molecular biologists and biochemists. All the main aspects of polyacrylamide gel electrophoresis are described, while valuable appendix materials bring the reader useful listings of suppliers' addresses, detection methods, and more.
General biochemistry --- gelelektroforese --- biochemie --- polyacrylamidegel --- proteïnen --- isoelectric focusing --- IEP (immunoelektrophorese) --- Gel electrophoresis --- Polyacrylamide gel electrophoresis --- Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Analysis --- Analyse --- Gel electrophoresis. --- Polyacrylamide gel electrophoresis. --- Analysis. --- Électrophorèse --- Electrophoresis --- Protéine --- proteins --- Technique analytique --- Analytical methods --- 577.112 --- 57.088 --- 543.545 --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- Elektroforese: proteïnen --- Basic Sciences. Chemistry --- Biochemistry --- Biochemistry. --- 543.545 Electrophoretic analysis. Zone electrophoresis. Ionophoresis. Electrochromatography --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- 577.112 Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Electrophoresis, Polyacrylamide gel --- PAGE (Biochemistry) --- Polyacrylamide disc electrophoresis --- Zone electrophoresis --- Proteins - Analysis. --- PAGE --- LABORATORY MANUALS --- PROTEINS --- GEL ELECTROPHORESIS --- METHODS
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DNA. --- Electrophoresis, Polyacrylamide Gel. --- Nucleoproteins. --- RNA. --- Gene Products, RNA --- Non-Polyadenylated RNA --- RNA, Non-Polyadenylated --- Ribonucleic Acid --- Acid, Ribonucleic --- Non Polyadenylated RNA --- RNA Gene Products --- RNA, Non Polyadenylated --- Biopolymers --- Nucleoprotein --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- ds-DNA --- DNA, Double-Stranded --- Deoxyribonucleic Acid --- DNA, Double Stranded --- Double-Stranded DNA --- ds DNA --- Analytical biochemistry --- Proteins --- Gel electrophoresis. --- Polyacrylamide gel electrophoresis. --- Analysis. --- Elektroforese. --- Nucleinezuren. --- DNA --- Electrophoresis, Polyacrylamide Gel --- Nucleoproteins --- RNA --- Amino acid sequence --- Rna --- GEL ELECTROPHORESIS --- NUCLEIC ACIDS --- ANALYSIS
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Gel electrophoresis --- Polyacrylamide gel electrophoresis --- Proteins --- Electrophorèse en gel --- Electrophorèse sur gel de polyacrylamide --- Protéines --- Analysis --- Analyse --- Electrophoresis, Polyacrylamide gel --- Immunoelectrophoresis --- Electrophoresis, Polyacrylamide Gel --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- isolation & purification --- Electrophoresis, Polyacrylamide Gel. --- Polyacrylamide Gel Electrophoresis --- SDS-PAGE --- Sodium Dodecyl Sulfate-PAGE --- Gel Electrophoresis, Polyacrylamide --- SDS PAGE --- Sodium Dodecyl Sulfate PAGE --- Sodium Dodecyl Sulfate-PAGEs --- Polyacrylamide gel electrophoresis. --- Electrophorèse en gel d'acrylamide --- Immunoélectrophorèse --- Proteins. --- 577.112 --- 577.112 Proteins --- Gene Products, Protein --- Gene Proteins --- Protein Gene Products --- Proteins, Gene --- Molecular Mechanisms of Pharmacological Action --- 57.088 --- 57.088 Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Special methods and techniques for studing biological molecules. Separation. Centrifuging. X-ray study. Radioisotope methods --- Analytical biochemistry --- Eiwitten. --- Elektroforese. --- methods --- laboratory manuals. --- Protein --- Electrophoresis, Polyacrylamide Gel - laboratory manuals --- Proteins - laboratory manuals
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The preparation of gene probes is a core application in molecular biology, creating essential tools for genetic research. A companion volume, Gene Probes 2, covers applications of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.
Molecular biology --- DNA probes. --- Sondes ADN --- Basic Sciences. Genetics --- Genetics (General) --- Genetics (General). --- GENES --- MOLECULAR BIOLOGY --- PROBES --- TECHNIQUES --- LABORATORY MANUALS --- USES
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This new volume, written in a highly readable style by internationally recognized experts, provides up-to-date information on a number of rapidly growing areas of developmental molecular biology. Carefully organized for greater ease of use, it includes a comparative review of the early development of a number of representative organisms, as well as recent advances in the normal and abnormal expression of key regulatory genes, and their effects on the developing embryo.
Biologie [Moleculaire ] --- Biologie moléculaire --- Biology [Molecular ] --- Egg (Biology) --- Embryogeny --- Embryologie --- Embryology --- Genetic regulation --- Genetische regulatie --- Moleculaire biologie --- Molecular biochemistry --- Molecular biology --- Molecular biophysics --- Régulation génétique --- Zoology--Embryology --- EMBRYOLOGY --- GENE EXPRESSION --- MOLECULAR BIOLOGY --- Embryology. --- Genetic regulation. --- Molecular biology. --- Monograph
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Research progress on the molecular biology of the immune system has been particularly rapid. This unique volume brings together five reviews by leading researchers on all major aspects of work in this area. These exciting advances have major implications not only for immunologists, but for all researchers studying gene structure and function.
Immunochemistry --- Molecular Biology --- Immunochemistry. --- Immunology. Immunopathology --- Molecular Biology.
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Chemistry, Organic --- Chimie organique --- Outlines, syllabi, etc --- Résumés, programmes, etc. --- 547 --- Organic chemistry --- 547 Organic chemistry --- Résumés, programmes, etc. --- ORGANIC CHEMISTRY --- STUDY AND TEACHING (HIGHER)
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This second edition of "Protein Structure" presents electrophoretic, chromatographic, and spectrophotometric techniques as well as including new chapters on the relatively sophisticated methods of mass spectrometry and ultracentrifugation. The emphasis is not on the technical aspects of the instruments, but more on the practical aspects of sample preparation and the possibilities of experimental strategy and design. The companion volume to this book, Protein Function: A Practical Approach, details comparable simple techniques for characterizing protein function. Topics in this second volume include: protein molecular weight determination by SDS-PAGE; protein analysis by mass spectrometry; immunological detection of proteins of known sequence; identification of common post-translational modifications; peptide mapping; counting integral numbers of residues by chemical modification; disulfide bonds between cysteine residues; analysis of protein conformation by gel electrophoresis; hydrodynamic properties of proteins; determining the molar absorbance coefficient of a protein; optical spectroscopy to characterize protein conformation and conformational changes; measuring the conformational stability of a protein; imunochemical analysis of protein conformation; stabilization of protein structure by solvents. Protein Structure: A Practical Approach will prove invaluable to all researchers, from PhD students to experienced group leaders.
Proteins --- Biochemistry --- Analysis. --- Structure. --- Technique. --- analysis. --- Protéines --- Biochimie --- Analyse --- Structure --- Technique --- analysis
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