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Three-Dimensional Electron Microscopy of Macromolecular Assemblies is the first systematic introduction to single-particle methods of reconstruction. It covers correlation alignment, classification, 3D reconstruction, restoration, and interpretation of the resulting 3D images in macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods.Key Features* Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized* Describes methods that have bee

Electron microscopy. --- Three-dimensional imaging in biology. --- Three-dimensional biological imaging --- Imaging systems in biology --- Microscopy --- THREE-DIMENSIONAL IMAGING IN BIOLOGY --- BIOLOGICAL MACROMOLECULES --- ELECTRON MICROSCOPY --- LABORATORY MANUALS

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The concept of molecular machines in biology has transformed the medical field in a profound way. Many essential processes that occur in the cell, including transcription, translation, protein folding and protein degradation, are all carried out by molecular machines. This volume focuses on important molecular machines whose architecture is known and whose functional principles have been established by tools of biophysical imaging (X-ray crystallography and cryo-electron microscopy) and fluorescence probing (single-molecule FRET). This edited volume includes contributions from prominent scientists and researchers who understand and have explored the structure and functions of these machines. This book is essential for students and professionals in the medical field who want to learn more about molecular machines.

Biochemistry. --- Cell physiology. --- Electron microscopy. --- Microbiology. --- Molecular biology. --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Biomolecules --- Systems biology --- Microbial biology --- Biology --- Microorganisms --- Microscopy --- Cell function --- Cytology --- Physiology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Composition

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Three-dimensional imaging in biology. --- Electron microscopy. --- Imagerie tridimensionnelle en biologie --- Microscopie électronique

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Clinical medicine

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Economic geography --- European Union --- Urban policy --- Infrastructure (Economics) --- Urban economics. --- Politique urbaine --- Infrastructures de transport --- Economie urbaine --- Urban economics --- -Urban economics --- -Cities and state --- Urban problems --- City and town life --- Economic policy --- Social policy --- Sociology, Urban --- City planning --- Urban renewal --- Cities and towns --- City economics --- Economics of cities --- Economics --- Capital, Social (Economics) --- Economic infrastructure --- Social capital (Economics) --- Social infrastructure --- Social overhead capital --- Economic development --- Human settlements --- Public goods --- Public works --- Capital --- Economic aspects --- -Infrastructure (Economics) --- -Cities and towns --- Cities and state --- Villes --- Urban policy - European Union countries --- Infrastructure (Economics) - European Union countries