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This paper presents the first causal evidence on the effects of school accountability systems on teacher labor markets. We exploit a 2002 change in Florida's school accountability system that exogenously shocked some schools to higher accountability grades and others to lower accountability grades, and measure whether teachers in shocked schools are more or less likely to move. Using microdata from the universe of Florida public school teachers, we find strong evidence that accountability shocks influence the teacher labor market; specifically, teachers are more likely to leave schools that have been downward shocked -- especially to the bottom grade -- and they are less likely to leave schools that have been upward shocked. We also find that accountability shocks influence the distribution of the measured quality of teachers (in terms of value added measures) who stay and leave their school, though the average differences are not large.
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Plant viruses impose a serious threat on agriculture, which motivates extensive breeding efforts for viral resistant crops and inspires lasting interests on basic research to understand the mechanisms underlying plant immunity against viruses. Viruses are obligate intracellular parasites. Their genomes are usually small and only encode a few products that are essential to hijack host machinery for their nucleotide and protein biosynthesis, and that are necessary to suppress host immunity. Plants evolved multilayers of defense mechanisms to defeat viral infection. In this research topic, we gathered 13 papers covering recent advances in different aspects of plant immunity against viruses, including reviews on RNA silencing and R gene based immunity and their application, translational initiation factor mediated recessive resistance, genome editing based viral immunity, role of chloroplast in plant-virus interaction, and research articles providing new mechanistic insights on plant-virus interactions. We hope that this Research Topic helps readers to have a better understanding of the progresses that have been made recently in plant immunity against viruses. A deeper understanding of plant antiviral immunity will facilitate the development of innovative approaches for crop protections and improvements.
Virus --- Hormone --- Plant Immunity --- NBS-LRR --- Soybean mosaic virus --- Cucumber mosaic virus --- Genome editing --- RNA silencing --- Chloroplast --- Recessive Resistance
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Plant viruses impose a serious threat on agriculture, which motivates extensive breeding efforts for viral resistant crops and inspires lasting interests on basic research to understand the mechanisms underlying plant immunity against viruses. Viruses are obligate intracellular parasites. Their genomes are usually small and only encode a few products that are essential to hijack host machinery for their nucleotide and protein biosynthesis, and that are necessary to suppress host immunity. Plants evolved multilayers of defense mechanisms to defeat viral infection. In this research topic, we gathered 13 papers covering recent advances in different aspects of plant immunity against viruses, including reviews on RNA silencing and R gene based immunity and their application, translational initiation factor mediated recessive resistance, genome editing based viral immunity, role of chloroplast in plant-virus interaction, and research articles providing new mechanistic insights on plant-virus interactions. We hope that this Research Topic helps readers to have a better understanding of the progresses that have been made recently in plant immunity against viruses. A deeper understanding of plant antiviral immunity will facilitate the development of innovative approaches for crop protections and improvements.
Virus --- Hormone --- Plant Immunity --- NBS-LRR --- Soybean mosaic virus --- Cucumber mosaic virus --- Genome editing --- RNA silencing --- Chloroplast --- Recessive Resistance
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Plant viruses impose a serious threat on agriculture, which motivates extensive breeding efforts for viral resistant crops and inspires lasting interests on basic research to understand the mechanisms underlying plant immunity against viruses. Viruses are obligate intracellular parasites. Their genomes are usually small and only encode a few products that are essential to hijack host machinery for their nucleotide and protein biosynthesis, and that are necessary to suppress host immunity. Plants evolved multilayers of defense mechanisms to defeat viral infection. In this research topic, we gathered 13 papers covering recent advances in different aspects of plant immunity against viruses, including reviews on RNA silencing and R gene based immunity and their application, translational initiation factor mediated recessive resistance, genome editing based viral immunity, role of chloroplast in plant-virus interaction, and research articles providing new mechanistic insights on plant-virus interactions. We hope that this Research Topic helps readers to have a better understanding of the progresses that have been made recently in plant immunity against viruses. A deeper understanding of plant antiviral immunity will facilitate the development of innovative approaches for crop protections and improvements.
Virus --- Hormone --- Plant Immunity --- NBS-LRR --- Soybean mosaic virus --- Cucumber mosaic virus --- Genome editing --- RNA silencing --- Chloroplast --- Recessive Resistance --- Virus --- Hormone --- Plant Immunity --- NBS-LRR --- Soybean mosaic virus --- Cucumber mosaic virus --- Genome editing --- RNA silencing --- Chloroplast --- Recessive Resistance
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TGEV infection has been reported in many countries around the world, causing highly contagious enteric disease in piglets. TGEV causes severe diarrhea, vomiting and dehydration in the piglets. The mortality rates of TGEV infection are high in pigs of all ages, reaching up to 100% in piglets less than 2 weeks old. Moreover the recovered piglets can also spread the virus to uninfected pig for many weeks.My thesis project aims to characterize a coronavirus membrane (M) protein. In particular the TGEV M protein acts as a major player in virus assembly and possibly interacts with host factors to modulate cellular functions.In the first part of my thesis, we prepared the monoclonal antibodies to TGEV N protein and identify the epitopes on TGEV N protein. The mAb (5E8) can be applied for different approaches to diagnosis of TGEV infection.In the second part of my thesis, we prepared the monoclonal antibodies to TGEV M protein and identify the epitopes on TGEV M protein. The mAb (1C3) can be used for the detection of the TGEV M protein in different assays. An indirect immunofluorescence assay (IFA) method for detection of TGEV M protein was optimized using mAb (1C3).In the third part of my thesis, cellular proteins interacting with TGEV M protein were screened by endogenous Co-IP coupled with MALDI-TOF/TOF MS. The cellular protein HSC70 was identified to interact with TGEV M directly. The endodomain of TGEV M and substrate binding domain (SBD) of HSC70 are essential for the interaction. HSC70 downregulation, as well as HSC70 ATPase activity inhibition, suppresses the TGEV replication. TGEV enters into the PK-15 cells by clathrin-mediated endocytosis, and interaction between M protein and HSC70 directs clathrin-mediated endocytosis of TGEV.In summary, my thesis contributed to a better understanding of TGEV infection.
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This paper presents the first causal evidence on the effects of school accountability systems on teacher labor markets. We exploit a 2002 change in Florida's school accountability system that exogenously shocked some schools to higher accountability grades and others to lower accountability grades, and measure whether teachers in shocked schools are more or less likely to move. Using microdata from the universe of Florida public school teachers, we find strong evidence that accountability shocks influence the teacher labor market; specifically, teachers are more likely to leave schools that have been downward shocked -- especially to the bottom grade -- and they are less likely to leave schools that have been upward shocked. We also find that accountability shocks influence the distribution of the measured quality of teachers (in terms of value added measures) who stay and leave their school, though the average differences are not large.
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