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This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Oxigen actiu --- Metabolisme --- Anabolisme --- Catabolisme --- Biologia --- Bioquímica --- Cicle de Krebs --- Gliconeogènesi --- Metabolisme basal --- Metabolisme cel·lular --- Metabolisme de les plantes --- Metabolisme dels lípids --- Metabolisme energètic --- Metabolisme microbià --- Metabolisme mineral --- Metabolisme secundari --- Urea --- Biosíntesi --- Nutrició --- Respiració --- Anions superòxid --- Oxigen reactiu --- Radicals d'oxigen --- Oxigen --- Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Química biològica --- Química fisiològica --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica clínica --- Bioquímica física --- Bioquímica quàntica --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química clínica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica

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In Functional Analysis of DNA and Chromatin, expert researchers in the field provide an overview of standard and more recent methods for the functional analysis of the genetic material. These include methods on DNA-dye binding modes, chromatin staining, nuclear dispersion assays, DNA labeling in vivo, sister chromatid exchanges, FISH, DNA and chromatin imaging by fluorescence, electron and atomic force microscopy, detection of apoptotic DNA, cytosine methylation and hydroxy-methylation, DNA thermophoresis, improved methods for histone analysis, chromatin immunoprecipitation, and analysis of rDNA genes and chromatin-associated RNA. Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Functional Analysis of DNA and Chromatin seeks to aid scientists in the further study of cellular and molecular biology of the nucleus.

Cytology. --- Human genetics. --- Cell Biology. --- Human Genetics. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology