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Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE). In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book
Capillary electrophoresis --- Carbohydrates --- High performance liquid chromatography --- Electrophorèse capillaire --- Chromatographie en phase liquide à hautes performances --- Analysis --- Capillary electrophoresis. --- High performance liquid chromatography. --- Chromatography, High Pressure Liquid --- Electrophoresis --- Glycoconjugates --- Analysis. --- analysis. --- methods. --- Electrophorèse capillaire --- Chromatographie en phase liquide à hautes performances --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- High performance chromatography --- High pressure liquid chromatography --- High sensitivity liquid chromatography --- High speed liquid chromatography --- HPLC (Chromatography) --- High pressure (Science) --- Liquid chromatography --- Carbs (Carbohydrates) --- Biomolecules --- Organic compounds --- Glycomics
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Carbohydrates --- Separation (Technology) --- Analysis. --- Analytical chemistry --- Chemistry, Technical --- Technology --- Carbs (Carbohydrates) --- Biomolecules --- Organic compounds --- Glycomics --- Chemical separations --- Separation processes --- Separation science --- Separation technologies --- Separations, Chemical
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This book is an updated and expanded edition of Carbohydrate Analysis, High Performance Liquid Chromatography and Capillary Electrophoresis and is concerned with the analysis of carbohydrates by modern chromatography and electrophoresis including analytical and preparative high performance liquid chromatography (HPLC), thin layer chromatography (TLC), field flow fractionation (FFF), capillary electrophoresis (CE), capillary electrochromatography (CEC), polyacrylamide gel electrophoresis (PAGE), gas chromatography (GC) and supercritical fluid chromatography (SFC). Thirty-one chapters c
Carbohydrates --- High performance liquid chromatography. --- Capillary electrophoresis. --- Capillary gel electrophoresis --- Capillary zone electrophoresis --- Gel electrophoresis --- Zone electrophoresis --- High performance chromatography --- High pressure liquid chromatography --- High sensitivity liquid chromatography --- High speed liquid chromatography --- HPLC (Chromatography) --- High pressure (Science) --- Liquid chromatography --- Carbs (Carbohydrates) --- Biomolecules --- Organic compounds --- Glycomics --- Analysis.
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Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE). In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book describes specialized methods for the separation, purification and detection of carbohydrates and glycoconjugates by HPLC and HPCE. Therefore, the objective of the present book is to provide a comprehensive review of carbohydrate analysis by HPLC and HPCE by covering analytical and preparative separation techniques for all classes of carbohydrates including mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in food and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc. The book will be of interest to a wide audience, including analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, etc. It will also be a useful reference work for both the experienced analyst and the newcomer as well as for users of HPLC and HPCE, graduates and postdoctoral students.
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This book is an updated and expanded edition of Carbohydrate Analysis, High Performance Liquid Chromatography and Capillary Electrophoresis and is concerned with the analysis of carbohydrates by modern chromatography and electrophoresis including analytical and preparative high performance liquid chromatography (HPLC), thin layer chromatography (TLC), field flow fractionation (FFF), capillary electrophoresis (CE), capillary electrochromatography (CEC), polyacrylamide gel electrophoresis (PAGE), gas chromatography (GC) and supercritical fluid chromatography (SFC). Thirty-one chapters cover: various modes of HPLC, CE, CEC, FFF, GC and SFC that are currently applied to the analysis of carbohydrates; discussions on analytical and preparative separations; descriptions of the principles of detection and quantitative determination of carbohydrates by the various separation techniques; reviews of sample preparations; and information on important applications. Furthermore, the book describes in detail the different direct and indirect detection methods that have been introduced for the sensitive detection of carbohydrates. This title is useful for a wide audience including separation scientists; analytical chemists and biochemists; carbohydrate chemists; glycoprotein and glycolipid chemists; molecular biologists; and biotechnologists. The book is also a useful reference for both the experienced analyst and the newcomer and for users of modern chromatography and electrophoresis. Contains 31 chapters covering all aspects of carbohydrate analysis by modern chromatography and electrophoresis Each chapter discusses the basic principles, advantages and limitations, and applications of the particular detection technique Useful reference for both the experienced analyst and the newcomer.
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