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Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.

Cytology. --- Gene expression. --- Cell Biology. --- Gene Expression. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Genes --- Genetic regulation --- Expression

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Plant gene silencing is a crucially important phenomenon in gene expression and epigenetics, and one of the most exciting discoveries of recent decades. This book brings together international experts to describe the science underlying it, and its applications in producing better plants. The book examines the detail of how transgenes induce post-transcriptional or trascriptional silencing. It also describes which genes are involved in these processes, and the production of small RNA molecules and their movement within plants. The involvement of viruses in silencing and factors affecting the hybrids used in agriculture are covered, along with silencing in fungi and algae. Strategies to produce artificial small RNAs are explained, with their use in improving industrial traits, adding nutritional benefits, removing allergens and other valuable factors for consumers. The book also explains how gene silencing can be used to protect plants against bacterial and viral pathogens, weeds and pests. Plant gene silencing is a fascinating biological subject with enormous potential and this is the first book to bring those aspects together.

Gene Silencing --- Gene Expression Regulation, Plant

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This book summarizes the latest findings on the functions of microRNAs in the regulation of plant development and responses to the surrounding environment. MicroRNAs are an important class of molecules that can be found in diverse groups of organisms, including plants and animals, and the investigation of their roles is a highly dynamic and “hot” research topic. The respective chapters address four main aspects, namely: microRNA investigation and annotation, the regulatory roles of microRNAs in various developmental processes, in response to abiotic factors, and in the context of biotic stress response regulation. Systematically reviewing the most important findings in this field, the book offers an essential guide for undergraduate and graduate students, teachers, and plant science researchers. Due to the potential applications of microRNAs in crop breeding and plant protection, it also represents a valuable resource for scientists in academia and the private sector alike. .

Plant physiology. --- Plant biochemistry. --- Plant anatomy. --- Plant development. --- Plant pathology. --- Plant Physiology. --- Plant Biochemistry. --- Plant Anatomy/Development. --- Plant Pathology. --- Plant genetics. --- Plants --- Genetics --- Botany --- Plant structure --- Structural botany --- Vegetable anatomy --- Anatomy --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics --- Physiology --- Communicable diseases in plants --- Crop diseases --- Crops --- Diseases of plants --- Microbial diseases in plants --- Pathological botany --- Pathology, Vegetable --- Phytopathology --- Plant pathology --- Vegetable pathology --- Agricultural pests --- Crop losses --- Diseased plants --- Phytopathogenic microorganisms --- Plant pathologists --- Plant quarantine --- Development of plants --- Plant development --- Developmental biology --- Growth (Plants) --- Structure --- Pathology --- Diseases and pests --- Diseases --- Wounds and injuries --- Ontogeny

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This book summarizes the latest findings on the functions of microRNAs in the regulation of plant development and responses to the surrounding environment. MicroRNAs are an important class of molecules that can be found in diverse groups of organisms, including plants and animals, and the investigation of their roles is a highly dynamic and “hot” research topic. The respective chapters address four main aspects, namely: microRNA investigation and annotation, the regulatory roles of microRNAs in various developmental processes, in response to abiotic factors, and in the context of biotic stress response regulation. Systematically reviewing the most important findings in this field, the book offers an essential guide for undergraduate and graduate students, teachers, and plant science researchers. Due to the potential applications of microRNAs in crop breeding and plant protection, it also represents a valuable resource for scientists in academia and the private sector alike. .

Plant physiology. Plant biophysics --- Phytomorphology. Phytoanatomy --- Biochemical engineering --- systematische plantkunde --- plantenfysiologie --- biochemie --- planten