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This volume aims at providing a complete and updated overview of gene downregulation in plants performed through virus-induced gene silencing (VIGS). Chapters guide readers through classical and newly developed protocols of VIGS to allow readers to initiate or optimize their own silencing experiments according to the methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Virus-Induced Gene Silencing in Plants: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Gene silencing --- Gene inactivation --- Inactivation, Gene --- Silencing, Gene --- Genetic regulation --- Botany. --- Plant genetics. --- Plant Science. --- Plant Genetics. --- Plants --- Genetics --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history
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This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, transcription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms.
Botany. --- Plant genetics. --- Plant Science. --- Plant Genetics. --- Plants --- Genetics --- Botanical science --- Floristic botany --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history
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Incontestablement, le terroir revêt aujourd’hui une connotation positive. C’est un signe de qualité recherché, aussi bien par les vignerons qui y voient une reconnaissance dans leurs pratiques des valeurs environnementales et paysagères ; par les consommateurs qui y trouvent une authenticité et une proximité avec les lieux de production ; mais aussi par les territoires pour lesquels le terroir devient facteur de développement. Mais ces valeurs positives du terroir ne sont que très récentes, il est donc nécessaire de déconstruire cette notion en mettant en miroir les acceptations disciplinaires du terroir. Chacun dans son rôle (du consommateur au vigneron), dans sa discipline (des sciences humaines et sociales aux sciences exactes), analyse, anticipe, interprète et définit des objets attachés à la vigne et au vin.
Geography --- Sociology --- paysage --- patrimoine --- AOC --- sociologie --- vin --- géographie --- alimentation --- qualité --- sciences sociales --- Terroir (viticulture) -- France --- Vin -- France
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