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Natural Killer (NK) cells are large granular lymphocytes of the innate immune system. They are widespread throughout the body, being present in both lymphoid organs and non-lymphoid peripheral tissues. NK cells are involved in direct innate immune reactions against viruses, bacteria, parasites and other triggers of pathology, such as malignant transformation, all of which cause stress in affected cells. Importantly, NK cells also link the innate and adaptive immune responses, contributing to the initiation of adaptive immune responses and executing adaptive responses using the CD16 FcgRIIIA immunoglobulin Fc receptor. Such responses are mediated through two major effector functions, the direct cytolysis of target cells and the production of cytokines and chemokines. The authors focus here on the nature of recognition events by NK cells and address how these events are integrated to trigger these distinct and graded effector functions.

Medicine. --- Immunology. --- Biomedicine. --- Immunobiology --- Life sciences --- Serology --- Clinical sciences --- Medical profession --- Human biology --- Medical sciences --- Pathology --- Physicians --- Killer cells. --- Lymphocytes. --- Cell receptors. --- Cell-mediated cytotoxicity. --- Cytotoxicity, Cell-mediated --- Cell death --- Killer cells --- Cell membrane receptors --- Cell surface receptors --- Receptors, Cell --- Binding sites (Biochemistry) --- Cell membranes --- Proteins --- Nongranular leucocytes --- Leucocytes --- K cells --- Natural killer cells --- NK cells --- Immunocompetent cells --- Cell-mediated cytotoxicity

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In Natural Killer Cell Protocols: Cellular and Molecular Methods, Kerry S. Campbell and Marco Colonna have assembled a comprehensive collection of readily reproducible methods designed to study natural killer (NK) cells from the broadest variety of viewpoints. These include not only classic techniques, but also new approaches to standard methods, newly evolved techniques that have become valuable for specific applications, and unique models for manipulating and studying NK cells. Among the advanced methods covered are those for in vitro transendothelial migration, in vivo detection of cells migrating into tumors, immunofluorescence staining of intracellular cytokines, and in vitro NK cell development. Valuable techniques for specific applications include vaccinia virus protein expression, soluble KIR-Fc fusions for HLA class I binding assays, calcium mobilization in cell conjugates, and identification of heterodimeric receptor complexes using cDNA library expression cloning. No less important are accounts of such classic methods as hybrid resistance, ADCC, viral defense, target cell cytotoxicity assays, cloning and culturing, tumor immunotherapy, and generation of HLA class I transfected target cells. Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.

Electronic books. -- local. --- Killer cells -- Immunology. --- Killer cells -- Laboratory manuals. --- Killer cells --- Killer Cells, Natural --- Cytotoxicity Tests, Immunologic --- Methods --- Lymphocytes --- Investigative Techniques --- Toxicity Tests --- Immunologic Tests --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Laboratory Techniques and Procedures --- Immunologic Techniques --- Leukocytes, Mononuclear --- Leukocytes --- Diagnosis --- Immune System --- Blood Cells --- Cells --- Hemic and Immune Systems --- Blood --- Anatomy --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- immunology. --- methods. --- Immunology. --- K cells --- Natural killer cells --- NK cells --- Immunocompetent cells --- Cell-mediated cytotoxicity --- Immunobiology --- Life sciences --- Serology

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