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Neisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors.  Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Neisseria meningitidis: Advanced Methods and Protocols can be readily used to study other pathogens and diseases and should have broad appeal to clinical and non-clinical scientists alike; however, there is still much to learn about the meningococcus and its complex nature, which in turn continues to reveal crucial details about human biology as well.

Emerging infectious diseases. --- Bacteriology. --- Infectious Diseases. --- Emerging infections --- New infectious diseases --- Re-emerging infectious diseases --- Reemerging infectious diseases --- Communicable diseases --- Microbiology

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This book reviews successes and (remaining) challenges in vaccine development for the selected Neglected Tropical Diseases (NTD) of Leprosy, Leishmaniasis, Meliodoisis and Tuberculosis, which are a continuous burden for millions of people in affected areas worldwide. Written by frontline researchers, the volume deep-dives into different vaccine strategies, provides biotechnological background information and also tackles animal models in NTD therapeutics research. By bringing together state-of-the-art expert knowledge, the book contributes to the aim of ultimately ending the epidemics of neglected tropical diseases, complying with UN Sustainable Development Goal 3, Health and Well-Being.The volume highlights the activities of the research network VALIDATE (VAccine deveLopment for complex Intracellular neglecteD pAThogEns), funded by the Medical Research Council in the UK. The four NTDs discussed in the book were selected as these are in the focus of VALIDATE’s research. The book targets scientists and clinicians working on NTDs, as well as all readers with a background in biomedicine and interest in vaccine development. This is an open access book.

Medical microbiology. --- Vaccines—Biotechnology. --- Biomaterials. --- Immunology. --- Biotechnology. --- Medical Microbiology. --- Biomaterials-Vaccines. --- Applied Immunology. --- Chemical engineering --- Genetic engineering --- Immunobiology --- Life sciences --- Serology

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