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Carbohydrate microarrays emerged as a key technology for the deciphering of the glycospace by providing a multiplex technology where tens to hundreds of carbohydrates/protein interactions can be probed in parallel. Carbohydrate Microarrays: Methods and Protocols aims to give the reader the theoretical and experimental clues necessary for the fabrication and implementation of carbohydrate microarrays. This requires three essential steps: 1) to obtain the carbohydrate probes (monosacharides, oligosacchrides, polysacchairdes, glycoconjugates or glycoclusters), 2) to immobilize these probes, and 3) to implement the protocols for biological/biochemical interaction with the desired target. This volume gives an overview of carbohydrate microarray and carbohydrate chemistry and illustrates different detection techniques and their applications. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Carbohydrate Microarrays: Methods and Protocols compiles a catalogue of protocols on carbohydrate microarrays to span the needs of researchers around the globe. .
Biochemistry. --- DNA microarrays. --- Protein Science. --- Microarrays. --- DNA biochips --- Microarrays, DNA --- Biochips --- Immobilized nucleic acids --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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Biomaterials repair, reinforce or replace damaged functional parts of the (human) body. All mechanical and biological interactions between an implant and the body occur across the interface, which has to correspond as nearly as possible to its particular function. Much of the progress in adapting polymer materials for use in a biological environment has been obtained through irradiation techniques. For this reason the most recent developments in four key areas are reviewed in this special volume: (1) the analysis of the topology and the elemental composition of a functional surface, (2) the chemical modification of the surface which results in highly pure, sterile and versatile surfaces, (3) the sterilisation of implantable devices via ionising radiation and its possible effects on the structural mechanical properties of polymers, and (4) the radiation effects on living cells and tissues which are of particular importance for radiation protection and radiotherapy.
Polymers in medicine. --- Polymers --- Effect of radiation on. --- Biochemistry. --- Biomedical engineering. --- Polymers . --- Condensed matter. --- Physical chemistry. --- Analytical chemistry. --- Biochemistry, general. --- Biomedical Engineering and Bioengineering. --- Polymer Sciences. --- Condensed Matter Physics. --- Physical Chemistry. --- Analytical Chemistry. --- Analysis, Chemical --- Analytic chemistry --- Chemical analysis --- Chemistry, Analytic --- Chemistry --- Chemistry, Theoretical --- Physical chemistry --- Theoretical chemistry --- Condensed materials --- Condensed media --- Condensed phase --- Materials, Condensed --- Media, Condensed --- Phase, Condensed --- Liquids --- Matter --- Solids --- Polymere --- Polymeride --- Polymers and polymerization --- Macromolecules --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Composition --- Polymers - Effect of radiation on.
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