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This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Clonatge molecular --- Seqüència de nucleòtids --- Bioinformàtica --- Informàtica biològica --- Ciències de la informació --- Biologia computacional --- Anàlisi seqüencial d'àcids nucleics --- Seqüència ADN --- Seqüència de l'ADN --- Seqüències de nucleòtids --- Seqüències dels nucleòtids --- Codi genètic --- Clonació molecular --- Clonatge de molècules --- Clonatge de gens --- Clonatge d'ADN --- Clonatge --- Enginyeria genètica --- Genètica molecular --- Anticossos monoclonals --- Vectors genètics --- Medical genetics. --- Medical Genetics. --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects