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This highly practical book, and successor to Volume 13 in the Laboratory Techniques series, explores further and provides more comprehensive, autoritative information on the production of Mabs. Much new and illuminating material has been included covering the concepts behind the application of recombinant DNA technology and biosensor technology to monoclonal antibodies, and all the human Mab technology facilitated by PCR of antibody genes. Also included in this latest volume is a section focussing on other methods of obtaining B cell clones such as short-term culture and oncogene tr

Biological techniques --- Immunology. Immunopathology --- Biotechnology --- Monoclonal antibodies. --- Biosensors. --- Biotechnology. --- Chemical engineering --- Genetic engineering --- Biodetectors --- Biological detectors --- Biological sensors --- Biomedical detectors --- Biomedical sensors --- Detectors --- Medical instruments and apparatus --- Physiological apparatus --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Hybridomas --- Immunologic Techniques --- 57.083.3 --- 57.083.3 Immunological methods and techniques --- Immunological methods and techniques --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Immunity --- Immunochemistry --- Hybridoma --- Interleukin-6 --- Monoclonal Antibodies --- Monoclonal Antibody --- Antibody, Monoclonal --- methods --- Chromatography, Gel --- 543.544 --- 661.18 --- Biochemistry --- -Gel permeation chromatography --- Molecular biology --- -Molecular biochemistry --- Molecular biophysics --- Biophysics --- Biomolecules --- Systems biology --- Chromatography, Gel permeation --- Chromatography, Size exclusion --- Gel filtration chromatography --- SEC (Chromatographic analysis) --- Size exclusion chromatography --- Steric exclusion liquid chromatography --- Chromatographic analysis --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- 661.18 Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- Colloids. Surface-active agents. Washing, foaming, flotation agents etc. --- 543.544 Chromatographic analysis. Chromatography --- Chromatographic analysis. Chromatography --- Chromatography, Size Exclusion --- Exclusion Chromatography --- Gel Chromatography --- Gel Permeation Chromatography --- Molecular Sieve Chromatography --- Chromatography, Exclusion --- Chromatography, Gel Permeation --- Chromatography, Molecular Sieve --- Gel Filtration --- Gel Filtration Chromatography --- Chromatography, Gel Filtration --- Exclusion Chromatography, Size --- Filtration Chromatography, Gel --- Filtration, Gel --- Sieve Chromatography, Molecular --- Size Exclusion Chromatography --- Technique --- Composition --- -Technique --- Enzymology --- Clinical chemistry --- Fysicochemical separation methods --- Analytical biochemistry --- Histology. Cytology --- Radioimmunoassay.

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"Ever since the archaeological rediscovery of the ancient Near East, generations of scholars have attempted to reconstruct the 'real Babylon,' known to us before from the evocative biblical account of the Tower of Babel. After two centuries of excavations and scholarship, Mario Liverani provides an insightful overview of modern, Western approaches, theories, and accounts of the ancient Near Eastern city"--Provided by publisher.

Cities and towns, Ancient --- Excavations (Archaeology) --- Babylon (Extinct city) --- Middle East --- Antiquities. --- Babylon (Extinct city). --- Babylon (Ancient city) --- History. --- Historiography. --- Communities - Urban Groups --- Sociology & Social History --- Social Sciences --- Geography, Ancient --- Iraq --- Antiquities --- Cities and towns, Ancient - Middle East. --- Excavations (Archaeology) - Middle East. --- Middle East - Antiquities. --- Babylon. --- Near East. --- cities. --- historiography.

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This volume contains detailed, comprehensive advice on rat, mouse and human hybridoma production. It begins with a general introduction, then describes the practical applications of the technology with photographs and protocols for everything from animal dissection to epitope analysis of antigens.

Biological techniques --- Molecular biology --- Immunology. Immunopathology --- Biochemical engineering

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