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Stress is a phenomenon that infiltrates our society in numerous ways. Apart from the daily life, stress is also present in the sports world and can have a considerable impact on the performance of even the best athletes. Especially for professional athletes, mastering stress management is of paramount importance to enable access to their full potential. This research aims to investigate the link between stress and performance in golf sport, more in particular during putting. Six elite athletes performed a pressured putting exercise while wearables were continuously monitoring their stress levels. The goal of the exercise consisted of making five consecutive putts. While several physiological variables were measured, heart rate proved to contain the most valuable information regarding the stress response. Two out of six athletes (athletes A and F) experienced the expected build-up of stress, expressed by the strong and significant correlations between the mean heart rate before the putt and the number of consecutively made putts (rA,1= 0,6427; rA,2= 0,7165; rF,1= 0,7057; rF,2= 0,9227). Other athletes only experienced the expected stress build-up when adding an additional stressor, being a reward for the team that finished the exercise first. Yet other athletes did not seem to experience the stress build-up in any case. This does not exclude that they may have experienced constant stress. When analysing the performance, all athletes showed a remarkable decrease in performance when there was a reward at stake. The increased pressure might have re- sulted in stress levels that were too high for optimal performance. Additionally, some individual zones of optimal functioning could be determined with acceptable accuracy (e.g. 81,25%). To enable generalization of these results to optimize an athlete’s performance, larger data sets and reliable baseline measurements are recommended. Lastly, multivariate classifiers revealed some individual optimal configurations of heart rate and breathing rate. However, more insight into when the athletes apply breathing relaxation exercises might help to understand the diverging individual optimal configurations.