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This volume focuses on stem cell research and disease modeling in non-murine species. The book is divided into three parts: Stem Cells for Pre-Clinical Models, Stem Cells in Non-Conventional Species, and Stem Cell Banking for the Future. The first section presents an overview of the different pre-clinical stem cell models recently created in animal species, including the porcine model for heart failure, iPSC in large animal species, Duchenne muscular dystrophy and canine embryo-derived stem cells and modeling for human diseases. This section also discusses the potential advantages and applications of these models. The second part of this book describes recent efforts to use stem cells for preserving endangered species, including the snow leopard and coral reefs. From this perspective, stem cells are an invaluable tool to preserve bio-diversities. Frozen cells and gametes can be obtained from animals at risk of extinction and even from microorganisms and corals suffering from heavy changes in the eco-system; this may allow the cultivation of a generation of stem cell lines and represents an exciting opportunity to support and ensure the conservation of precious varieties of living creatures. This discussion leads easily into the third section, which discusses stem cell banking as a way of safeguarding these endangered species.

Stem cells --- Research. --- Stem cells. --- Biochemistry. --- Stem Cells. --- Regenerative Medicine/Tissue Engineering. --- Animal Biochemistry. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Cells --- Composition --- Regenerative medicine. --- Tissue engineering. --- Biomedical engineering --- Regenerative medicine --- Tissue culture --- Medicine --- Regeneration (Biology)

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This addition to the Springer Brief in Stem Cells series focuses on aspects related to the specific mechanisms that ensure and control pluripotency and cell commitment in domestic animal species. This topic is generating rapidly-increasing interest due to the great potential for domestic animal species to be used as intermediate biomedical models, between the mouse and the human. The Brief addresses why we need large animal models for regenerative medicine. It also describes early embryo development with a careful and specific analysis of the regulatory mechanisms driving cleavage, polarization and genome activation in domestic species. How pluripotency is compartmentalized in domestic species as well as the different aspects that make the derivation of stem cells in domestic species very difficult are also addressed.

Biology --- Health & Biological Sciences --- Cytology --- Animal experimentation --- Medicine --- Animal models in research --- Research --- Life sciences. --- Reproductive medicine. --- Cell biology. --- Stem cells. --- Embryology. --- Life Sciences. --- Stem Cells. --- Cell Biology. --- Reproductive Medicine. --- Biological models --- Laboratory animals --- Cytology. --- Human reproduction --- Human reproductive health --- Human reproductive medicine --- Reproductive medicine --- Health --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Animal embryology --- Animals --- Development, Embryological --- Development, Embryonic --- Development, Zygotic --- Embryogenesis --- Embryogeny --- Embryological development --- Embryonic development --- Zoology --- Zygote development --- Zygotes --- Zygotic development --- Zygotic embryogenesis --- Developmental biology --- Morphology (Animals) --- Embryos --- Reproduction --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Health aspects --- Embryology --- Development --- Health Workforce

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This Brief offers a concise, handy overview of the main concepts related to Embryology, revisited through the novel concepts that are applied daily in stem cell research and cell therapy oriented investigations. It is based on three main areas:   -The process involved in female gamete differentiation and maturation. The main aspects related to cell biology will be covered and an overview of the epigenetic regulation of gametogenesis will be presented.   -Early stages of embryo development with a careful analysis of the regulatory mechanisms driving cleavage, polarization and genome activation. -Stem cell and gametogenesis. The use of the oocyte as a possible source for the derivation of stem cell lines is discussed and depicted as a powerful tool to investigate oocyte potency and asymmetric imprinting. The potential biological  implications are evaluated and use of stem cells to derive oocytes is presented. .

Histology. Cytology --- Biology --- Gynaecology. Obstetrics --- stamcellen --- biologie --- cytologie --- vroedkunde --- stamceltransplantatie --- histologie

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This detailed book collects original protocols aimed at encouraging and stimulating the scientific community to design and produce models for the laboratory that mimic cell guidance conditions as they occur in vivo. The protocols collected describe powerful strategies to exploit chemical cues involved in cell differentiation processes. Special emphasis is given to the use of methods for purification and characterization of exosomes and other secreted vesicles, as well as micro and non-coding RNAs, that have been demonstrated to control the tuning of the in vivo micro and macro environment in order to ensure the optimal soluble environment in vitro. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Next Generation Culture Platforms for Reliable In Vitro Models: Methods and Protocols serves as an ideal guide for researchers working toward developing these vital testing models for preclinical studies.

Cultiu de teixits --- Cultiu de teixits in vitro --- Teixits (Histologia) --- Enginyeria de teixits --- Transformació cel·lular --- Cell differentiation. --- Regenerative medicine. --- Cell Differentiation. --- Regenerative Medicine and Tissue Engineering. --- Medicine --- Regeneration (Biology) --- Cell fate specification --- Cell specification --- Cells --- Differentiation of cells --- Fate specification of cells --- Specification of cells --- Morphogenesis --- Differentiation --- Fate specification --- Specification --- Diferenciació cel·lular --- Citodiferenciació --- Diferenciació de les cèl·lules --- Histogènesi --- Morfogènesi --- Genètica del desenvolupament