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In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of bio

Enzymes. --- Basic Sciences. Chemistry --- Biochemistry --- Proteins and Enzymes. --- Biocatalysts --- Ferments --- Soluble ferments --- Catalysts --- Proteins --- Enzymology

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This unique, practical, pocket-sized guide and reference provides every first year bioscience student with all they need to know to prepare reagents correctly and perform fundamental laboratory techniques. It also helps them to analyse their data and present their findings, in addition to directing the reader, via a comprehensive list of references, to relevant further reading 

All of the core bioscience laboratory techniques are covered including: basic calculations and the preparation of solutions; aseptic techniques; microscopy techniques; cell fractionation ; spectrophotometry; chromatography of small and large molecules: electrophoresis of proteins and nucleic acids and data analysis. In addition the book includes clear, relevant diagrams and worked examples of calculations.


Fysicochemical separation methods --- Biological microscopy --- General embryology. Developmental biology --- General biochemistry --- Semiology. Diagnosis. Symptomatology --- ionenuitwisselingschromatografie --- affiniteitschromatografie --- biochemie --- microscopie --- electrophoresis --- LC (liquid chromatography) --- moleculaire biologie --- chromatografie --- DLC (dunnelaagchromatografie) --- HPLC (high power liquid chromatography) --- spectrofotometrie