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Transmembrane Signaling Protocols brings together a collection of practical, cutting-edge techniques for the study of cell signaling. Written by acknowledged leaders in the field, the protocols provide detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the latest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. The time-tested techniques collected in Transmembrane Signaling Protocols make available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. The book is destined to become a standard resource for both conceptual information and the practical detail needed to carry out and interpret laboratory procedures in this dramatically advancing field.

SCIENCE --- Life Sciences / Biochemistry --- Cellular signal transduction --- Signal Transduction --- Biochemical Processes --- Cell Physiological Processes --- Cell Physiological Phenomena --- Chemical Processes --- Biochemical Phenomena --- Chemical Phenomena --- Phenomena and Processes --- Animal Biochemistry --- Human Anatomy & Physiology --- Health & Biological Sciences --- Laboratory manuals --- Laboratory manuals. --- Cellular control mechanisms --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Information theory in biology --- Cell regulation --- Biological control systems --- Cell metabolism --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- CELLULAR SIGNAL TRANSDUCTION --- LABORATORY MANUALS