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This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product.  Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host.  A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production.  This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization. .

Life Sciences. --- Biological Techniques. --- Industrial and Production Engineering. --- Biomedicine general. --- Life sciences. --- Medicine. --- Cytology --- Industrial engineering. --- Sciences de la vie --- Médecine --- Génie industriel --- Research_xMethodology. --- Cytology_xResearch_xMethodology. --- Biology --- Health & Biological Sciences --- Biology - General --- Enzymes --- Protein engineering. --- Biotechnology. --- Engineering, Protein --- Protein design --- Proteins --- Design --- Production engineering. --- Technique. --- Biochemical engineering --- Genetic engineering --- Research --- Methodology. --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Management engineering --- Simplification in industry --- Engineering --- Value analysis (Cost control) --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Health Workforce --- Biology—Technique. --- Biomedicine, general. --- Manufacturing engineering --- Process engineering --- Industrial engineering --- Mechanical engineering

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This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization. .

Methodology of economics --- Biological techniques --- Human biochemistry --- Human medicine --- Engineering sciences. Technology --- Production management --- Business management --- Business economics --- Biotechnology --- medische biochemie --- biologische technieken --- financieel management --- geneeskunde --- biotechnologie --- productie --- ingenieurswetenschappen

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This contributed volume, "Multifaceted Protocols in Biotechnology, Volume 2", consists of multidisciplinary methods and techniques commonly used in biotechnology studies. There are two sections covered in this book - Ionic Liquid Related Techniques & Evergreen Biotechnology Techniques. A brief introduction supports each protocol to allow easy learning and implementation. The first section consists of three chapters covering studies in modern biotechnology focusing on the role of ionic liquid techniques in extracting secondary metabolites, enzyme stabilization and biomass processing. The second section covers evergreen methodologies. It comprises five chapters covering topics on microcarrier technology for cell culture; Polymerase Chain Reaction for non-halal sources detection in food; ELISA for biomarker identification; gamma ray-induced mutagenesis for enhancing microbial fuel cells; and the effect of temperature on antibacterial activity of Carica papaya seed extract. This book will be useful to graduate students, researchers, academics, and industry practitioners working in the area of biotechnology.

Human biochemistry --- Pathological biochemistry --- Human medicine --- Biotechnology --- medische biochemie --- bio-engineering --- biomedische wetenschappen --- biotechnologie

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Sociology of culture --- Sociology of minorities --- Industrial economics --- Pharmacology. Therapy --- Business policy --- Personnel management --- Food science and technology --- Information systems --- Computer. Automation --- Civil engineering. Building industry --- ICT (informatie- en communicatietechnieken) --- bedrijfseconomie --- farmacologie --- industrie --- voedingsleer --- intercultureel management --- culturele diversiteit --- informatica management

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This edited work presents useful methods in experimenting in the area of Bioprocessing and Biotechnology. The four sections cover the area of Bioprocess, Whole Cells & Isolated Biocatalyst, Characterization of Biochemical Products and Cell Isolation & Culture. Its enable researchers to choose a suitable method and plan their experiments in details. The main focus of this book is to provide step by step method to young researchers, especially in the new research areas. Among the latest areas are the isolation of novel strain or enzyme by metagenomic approaches and taming procedure in the laboratories, development of novel, the cheap and non-toxic catalyst for biodiesel production, and production of micro-fibrillated cellulose. An updated method for well-known areas such as immobilization technology, biosensor, and polymerization was also presented. The book also covers in-silico methods such as MATLAB platform to ease researchers. Not to forget, the method in animal and plant culture is also discussed in detail. The book is written by chapter authors with much expertise in their fields. They have published multiple articles in the index listed journals. The topic of this book is particularly relevant to young researchers who are struggling to fine-tune their research and do not want to waste their time in optimizing the experiment set up.

Chemical structure --- General biochemistry --- Matlab (informatica) --- protein-engineering --- biosensoren --- biochemie --- biotechnologie --- eiwitten --- polymeren --- moleculaire biologie --- enzymen