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Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.

Cell lines. --- Cell culture. --- Cultures (Biology) --- Cytology --- Cell culture --- Technique --- Medicine. --- Cytology. --- Genetic engineering. --- Biochemistry. --- Biomedicine general. --- Cell Biology. --- Genetic Engineering. --- Biochemistry, general. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Pathology --- Physicians --- Composition --- Cell biology. --- Biomedicine, general. --- Health Workforce --- Medicine --- Biotechnology. --- Biomedical Research. --- Chemical Bioengineering. --- Research. --- Chemical engineering --- Genetic engineering --- Biological research --- Biomedical research

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Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.

Immunoglobulins -- Biotechnology. --- Immunoglobulins. --- Immunology. --- Immunoglobulins --- Serum Globulins --- Immunoproteins --- Blood Proteins --- Globulins --- Proteins --- Amino Acids, Peptides, and Proteins --- Chemicals and Drugs --- Antibodies --- Biology --- Health & Biological Sciences --- Microbiology & Immunology --- Biotechnology --- Immune globulins --- Immune serum globulin --- Medicine. --- Biomedicine. --- Biomedicine general. --- Blood proteins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins --- Clinical sciences --- Medical profession --- Human biology --- Life sciences --- Medical sciences --- Pathology --- Physicians --- Health Workforce --- Biomedicine, general. --- Medicine --- Biomedical Research. --- Research. --- Biological research --- Biomedical research

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Integrating advances in molecular biology into bioprocesses presents a continuous challenge to scientists and bioengineers. This series is conceived to help meet this challenge. It examines and assesses the feasibility of new approaches for the modification of cellular function such as gene expression, protein processing, secretion, glycosylation, immortalisation, proliferation, and apoptosis as well as the systematic study of the metabolic genotype-phenotype relationship. The series provides detailed coverage of the methodology for improving cellular properties of cells used in the production of biopharmaceuticals, gene and cell therapies and tissue engineering. It also seeks to explain the cellular mechanisms underlying in vitro physiological activity and productivity. This volume, which is based on presentations at the `European Workshop on Animal Cell Engineering' held in Costa Brava, Spain, contains a collection of chapters relating to cellular function and modification by leading authorities in several different areas of basic research and the biopharmaceutical industry.

Animal cell biotechnology. --- Genetic engineering. --- Genetic regulation. --- Cell proliferation. --- Zoology. --- Biotechnology. --- Cytology. --- Morphology (Animals). --- Biomedical engineering. --- Pharmacy. --- Cell Biology. --- Animal Anatomy / Morphology / Histology. --- Biomedical Engineering and Bioengineering. --- Cell biology. --- Animal anatomy. --- Chemistry --- Medicine --- Drugs --- Materia medica --- Pharmacology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Animal anatomy --- Animals --- Biology --- Physiology --- Cell biology --- Cellular biology --- Cells --- Chemical engineering --- Genetic engineering --- Natural history --- Anatomy --- Animal cells in biotechnology --- Animal biotechnology --- Cell renewal --- Cellular proliferation --- Cell cycle --- Cell division --- Cell populations --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Gene expression --- Gene expression regulation --- Gene regulation --- Biosynthesis --- Cellular control mechanisms --- Molecular genetics --- Growth --- Regulation --- Anatomy, Comparative. --- Animal Anatomy.

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Histology. Cytology --- General biochemistry --- Molecular biology --- Human biochemistry --- medische biochemie --- biochemie --- cytologie --- genetische manipulatie --- histologie --- moleculaire biologie

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Animal cells are the preferred “cell factories” for the production of complex molecules and antibodies for use as prophylactics, therapeutics or diagnostics. Animal cells are required for the correct post-translational processing (including glycosylation) of biopharmaceutical protein products. They are used for the production of viral vectors for gene therapy. Major targets for this therapy include cancer, HIV, arthritis, cardiovascular and CNS diseases and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological studies. This book is designed to serve as a comprehensive review of animal cell culture, covering the current status of both research and applications. For the student or R&D scientist or new researcher the protocols are central to the performance of cell culture work, yet a broad understanding is essential for translation of laboratory findings into the industrial production. Within the broad scope of the book, each topic is reviewed authoritatively by experts in the field to produce state-of-the-art collection of current research. A major reference volume on cell culture research and how it impacts on production of biopharmaceutical proteins worldwide, the book is essential reading for everyone working in cell culture and is a recommended volume for all biotechnology libraries.

General embryology. Developmental biology --- Histology. Cytology --- Human biochemistry --- Human medicine --- medische biochemie --- geneeskunde --- cytologie --- stamceltransplantatie --- histologie --- celprocessen