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Molecular and cell morphological methods have increased rapidly in number and versatility in recent years and, with the completion of the human genome project, will come into much greater demand for the study of human genes and diseases. In Morphology Methods: Cell and Molecular Biology Techniques, Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. Valuable notes on potential pitfalls and practical tips designed to ensure success are included with each method. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today.

Cytology --- Fluorescence in situ hybridization. --- Immunohistochemistry. --- Molecular biology --- Polymerase chain reaction. --- Technique. --- Biological techniques --- Human histology. Human cytology --- Pathology. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Chain reaction, Polymerase --- PCR (Biochemistry) --- Polymerization --- DNA polymerases --- Immunohistology --- Histochemistry --- Immunochemistry --- FISH (Fluorescence in situ hybridization) --- Fluorescent in situ hybridization --- Fluorescence microscopy --- In situ hybridization

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In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

Antigens. --- Histochemistry. --- Immunohistochemistry. --- Neoplasms -- diagnosis. --- Noradrenaline. --- Immunohistochemistry --- Histocytochemistry --- Immunochemistry --- Immunologic Tests --- Biochemistry --- Laboratory Techniques and Procedures --- Cytological Techniques --- Histological Techniques --- Histology --- Immunologic Techniques --- Investigative Techniques --- Anatomy --- Diagnosis --- Chemistry --- Clinical Laboratory Techniques --- Biological Science Disciplines --- Natural Science Disciplines --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Disciplines and Occupations --- Biology --- Medicine --- Health & Biological Sciences --- Pathology --- Microbiology & Immunology --- Immunohistology --- Medicine. --- Pathology. --- Medicine & Public Health. --- Histochemistry --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine, Preventive

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This volume examines immunohistochemical methods for investigating the toxicologic pathology of rodent and other animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with an authoritative review of the methods of immunohistochemical staining in various target tissues. Further, this book explores the tissue-, antigen-, and antibody-specific problems that may be encountered during the staining procedures and provides potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as to image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents and other animal models. The information in Technical Aspects of Toxicological Immunohistochemistry is rounded out by the companion book, Toxicological Immunohistochemistry which examines mechanistic and predictive biomarkers for toxicological evaluation. .

Cytology --- Biology --- Health & Biological Sciences --- Immunohistochemistry. --- Immunotoxicology. --- Immunologic toxicology --- Immunotoxicity --- Immunohistology --- Immunopathology --- Immunopharmacology --- Toxicology --- Histochemistry --- Immunochemistry --- Cytology. --- Toxicology. --- Microscopy. --- Pathology. --- Cell Biology. --- Pharmacology/Toxicology. --- Biological Microscopy. --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Chemicals --- Pharmacology --- Poisoning --- Poisons --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Cell biology. --- Pharmacology. --- Drug effects --- Medical pharmacology --- Chemotherapy --- Drugs --- Pharmacy --- Physiological effect

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Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.

Human histology. Human cytology --- Biological microscopy --- Immunology. Immunopathology --- Microscopy. --- Electron microscopy. --- Antigens. --- Microscopie --- Microscopie électronique --- Antigènes --- 611.018 --- Histology. Histogenesis. Tissue structure, formation and differentiation --- Human anatomy. --- Immunohistochemistry. --- Life sciences. --- Medicine. --- Oncology. --- Pathology. --- Immunohistochemistry --- Microscopy --- Electron microscopy --- Antigens --- Histocytochemistry --- Immunologic Tests --- Immunochemistry --- Investigative Techniques --- Biological Factors --- Diagnostic Imaging --- Optics and Photonics --- Diagnostic Techniques and Procedures --- Histological Techniques --- Cytological Techniques --- Biochemistry --- Chemicals and Drugs --- Histology --- Laboratory Techniques and Procedures --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Physics --- Immunologic Techniques --- Clinical Laboratory Techniques --- Natural Science Disciplines --- Biological Science Disciplines --- Chemistry --- Diagnosis --- Anatomy --- Disciplines and Occupations --- Microbiology & Immunology --- Biology --- Health & Biological Sciences --- 611.018 Histology. Histogenesis. Tissue structure, formation and differentiation --- Medicine & Public Health. --- Anatomy. --- Life Sciences, general. --- Analysis, Microscopic --- Light microscopy --- Micrographic analysis --- Microscope and microscopy --- Microscopic analysis --- Optical microscopy --- Optics --- Immunohistology --- Histochemistry --- Immunity --- Immunoglobulins --- Oncology  . --- Biosciences --- Sciences, Life --- Science --- Disease (Pathology) --- Medical sciences --- Diseases --- Medicine --- Medicine, Preventive --- Tumors --- Anatomy, Human --- Human biology --- Human body

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Diagnostic immunohistochemistry --- Immunohistochemistry --- Immunologic Techniques --- Antibody Dissociation --- Immunologic Technic --- Immunologic Technics --- Immunologic Technique --- Immunological Technics --- Immunological Techniques --- Technic, Immunologic --- Technics, Immunologic --- Technique, Immunologic --- Techniques, Immunologic --- Antibody Dissociations --- Dissociation, Antibody --- Dissociations, Antibody --- Immunological Technic --- Immunological Technique --- Technic, Immunological --- Technics, Immunological --- Technique, Immunological --- Techniques, Immunological --- Allergy and Immunology --- Immunity --- Immunochemistry --- Immunogold Technics --- Immunogold-Silver Technics --- Immunolabeling Technics --- Immunocytochemistry --- Immunogold Techniques --- Immunogold-Silver Techniques --- Immunohistocytochemistry --- Immunolabeling Techniques --- Immunogold Silver Technics --- Immunogold Silver Techniques --- Immunogold Technic --- Immunogold Technique --- Immunogold-Silver Technic --- Immunogold-Silver Technique --- Immunolabeling Technic --- Immunolabeling Technique --- Technic, Immunogold --- Technic, Immunogold-Silver --- Technic, Immunolabeling --- Technics, Immunogold --- Technics, Immunogold-Silver --- Technics, Immunolabeling --- Technique, Immunogold --- Technique, Immunogold-Silver --- Technique, Immunolabeling --- Techniques, Immunogold --- Techniques, Immunogold-Silver --- Techniques, Immunolabeling --- Diagnostic immunohistology --- Immunodiagnosis --- Atlases --- methods