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biology --- biological sciences --- Biology --- Biology.
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This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practising cell culture will benefit greatly from this book.
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There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume.; The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions.
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Darwin consolidated a lifetime of work in
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Créé au sein d'un service de soin ou dans un cadre associatif, le groupe de parole a pour but d'aider et de soutenir les personnels soignants et les bénévoles. Il répond à l'objectif d'améliorer la relation soignant-soigné. Ni essai théorique, ni livre de recette, cet ouvrage constitue une somme d'indications pragmatiques, de références et de mises en garde pour conduire un groupe de parole. Qui peut s'engager sur cette voie? Pourquoi et comment le mettre en place? Comment en conduire le déroulement ?... Un livre de qualité sur une pratique très généralisée mais qui n'avait pas fait jusqu'ici l'objet d'une synthèse. Cette nouvelle présentation est accompagnée d'un complément en ligne qui présente un film documentaire sur les groupes de paroles animés par M. Ruszniewski réalisé par Gil Rabier.
Psychiatry --- Health & Biological Sciences --- Psychotherapy
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