Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. .

Biochemistry. --- Protein Science. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition --- Proteïnes --- Estructura molecular --- Manuals de laboratori --- Tècniques de laboratori --- Estructura química --- Molècules --- Orbitals moleculars --- Estructura atòmica --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Myosins are molecular motors that use the energy from ATP hydrolysis to move and exert tension on actin filaments. Although the best-known myosin is myosin II, which powers skeletal muscle contraction, there are at least two dozen classes of myosins, and cells generally express multiple isoforms. Myosins are involved in multiple cellular activities including cell structure, cell migration, intracellular trafficking, and cell-cell contact. Importantly, loss of function and mutation are associated with diseases including myopathies, hearing impairment, glomerulosclerosis, and cancer. Written by international experts in myosin motors and the approaches used to study them, this book is expected to provide a comprehensive assessment of the current status of our understanding of the structure and molecular mechanism of myosins and their cellular roles.

Myosin. --- Microfilament proteins --- Muscle proteins --- Molecular biology. --- Proteins . --- Cell biology. --- Molecular Medicine. --- Protein Science. --- Cell Biology. --- Protein Structure. --- Cell biology --- Cellular biology --- Biology --- Cells --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Systems biology --- Proteïnes --- Estructura molecular --- Citologia --- Biologia cel·lular --- Biologia --- Citogenètica --- Citoquímica --- Migració cel·lular --- Cèl·lules --- Cèl·lules animals --- Ultraestructura (Biologia) --- Estructura química --- Molècules --- Orbitals moleculars --- Estructura atòmica --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

Protein homeostasis, or “Proteostasis”, lies at the heart of human health and disease. From the folding of single polypeptide chains into functional proteins, to the regulation of intracellular signaling pathways, to the secreted signals that coordinate cells in tissues and throughout the body, the proteostasis network operates to support cell health and physiological fitness. However, cancer cells also hijack the proteostasis network and many of these same processes to sustain the growth and spread of tumors. The chapters in this book are written by world experts in the many facets of the proteostasis network. They describe cutting-edge insights into the structure and function of the major chaperone and degradation systems in healthy cells and how these systems are co-opted in cancer cells and the cells of the tumor microenvironment. The chapters also cover therapeutic interventions such as the FDA-approved proteasome inhibitors Velcade and Krypolis as well as other therapies currently under clinical investigation to disarm the ability of the proteostasis network to support malignancy. This compendium is the first of its kind and aims to serve as a reference manual for active investigators and a primer for newcomers to the field. This book is dedicated to the memory of Susan Lindquist, a pioneer of the proteostasis field and a champion of the power of basic scientific inquiry to unlock the mechanisms of human disease. The chapter “Reflections and Outlook on Targeting HSP90, HSP70 and HSF1 in Cancer: A Personal Perspective” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Human genetics. --- Cancer research. --- Molecular biology. --- Proteomics. --- Proteins . --- Human Genetics. --- Cancer Research. --- Molecular Medicine. --- Protein Science. --- Protein Structure. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Molecular biology --- Proteins --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Systems biology --- Cancer research --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Molecular chaperones. --- Chaperone proteins --- Chaperones, Molecular --- Chaperonins --- Proteïnes --- Oncologia --- Medicina --- Oncologia geriàtrica --- Oncologia pediàtrica --- Oncologia veterinària --- Càncer --- Infermeria oncològica --- Tumors --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The book focuses on the current research of the relation between protein phosphorylation and meat quality, reviews the influence mechanism of protein phosphorylation on meat quality, and summarizes the improvement of meat quality by regulating protein phosphorylation. It could help to clarify some dilemmas and encourage further research in this field, aiming for effective application of protein phosphorylation in meat quality in the near future. The book is written for researchers and graduate students in the field of meat science, food chemistry and molecular biology etc.

Food—Biotechnology. --- Proteins . --- Food Science. --- Protein Science. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Meat --- Quality. --- Meat quality --- Quality of products --- Fosforilació --- Proteïnes --- Carn --- Qualitat dels aliments --- Qualitat alimentària --- Qualitat dels productes --- Control de qualitat dels aliments --- Aliments --- Aliments d'origen animal --- Productes d'origen animal --- Anyell (Carn) --- Carn d'aviram --- Carn de boví --- Carn de porc --- Productes carnis --- Cuina (Carn) --- Indústria càrnia --- Biomolècules --- Albúmines --- Apoproteïnes --- Conformació de proteïnes --- Enzims --- Factors de transcripció --- Glicoproteïnes --- Lipoproteïnes --- Nucleoproteïnes --- Pèptids --- Prions --- Protamines --- Proteïnes citosquelètiques --- Proteïnes de membrana --- Proteïnes de la sang --- Proteïnes recombinants --- Proteïnes supressores de tumors --- Receptors cel·lulars --- Ubiqüitina --- Xaperones moleculars --- Proteòmica --- Metabolisme dels glúcids --- Reaccions químiques

Choose an application

• Reference Manager
• EndNote
• RefWorks (Direct export to RefWorks)

The revolutionary discoveries of RNA interference and bacterial regulatory RNAs led to further breakthroughs such as the identification of riboswitches and related RNA sensors, mRNA regions capable of alternating their conformations in response to the presence of cellular metabolites and other physical or chemical cues. In Riboswitches: Methods and Protocols, expert researchers provide comprehensive and up-to-date coverage of various methods used to study riboswitches and other RNAs involved in gene expression control. Examining biochemical and modern biophysical techniques, the volume focuses on mRNAs responding to small organic molecules but expands the definition of a riboswitch to incorporate classes of RNA that undergo conformational transitions in response to other stimuli in order to control the expression of genes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Riboswitches: Methods and Protocols promises to inspire both novices and experts working in a wide range of contemporary biological areas who wish to further develop their RNA methodology.

Biochemistry. --- Nucleic acids. --- Gene expression. --- Biochemistry, general. --- Nucleic Acid Chemistry. --- Gene Expression. --- Polynucleotides --- Biomolecules --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Genes --- Genetic regulation --- Composition --- Expression --- RNA --- Àcids nucleics --- Bioquímica --- Química biològica --- Química fisiològica --- Biologia --- Medicina --- Química --- Absorció (Fisiologia) --- Anàlisi enzimàtica --- Biodegradació --- Biodisponibilitat --- Bioelectroquímica --- Bioenergètica --- Biogeoquímica --- Biologia molecular --- Bioquímica analítica --- Bioquímica clínica --- Bioquímica física --- Bioquímica quàntica --- Biosíntesi --- Centres actius (Bioquímica) --- Citoquímica --- Composició del cos humà --- Cromatografia --- Digestió --- Enginyeria bioquímica --- Enzimologia --- Equilibri àcid-base --- Fitoquímica --- Fixació de proteïnes --- Genètica bioquímica --- Immunoquímica --- Interfícies biològiques --- Lípids --- Lligands (Bioquímica) --- Marcadors bioquímics --- Metabolisme --- Minerals en l'organisme --- Neuroquímica --- Oxidació fisiològica --- Pigments (Biologia) --- Química biorgànica --- Química clínica --- Química microbiològica --- Quimiotaxi --- Quimiotaxonomia --- Toxicologia bioquímica --- Xenobiòtics --- Histoquímica --- Biomolècules --- ADN --- Nucleoproteïnes --- Transformació genètica --- Àcid ribonucleic --- ARN --- Micro RNAs