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Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Blood platelets --- Megakaryocytes --- Blood platelets -- Laboratory manuals. --- Electronic books. -- local. --- Megakaryocytes -- Laboratory manuals. --- Blood Cells --- Clinical Laboratory Techniques --- Bone Marrow Cells --- Biological Science Disciplines --- Blood --- Cells --- Investigative Techniques --- Natural Science Disciplines --- Hematopoietic System --- Analytical, Diagnostic and Therapeutic Techniques and Equipment --- Anatomy --- Hemic and Immune Systems --- Disciplines and Occupations --- Blood Platelets --- Physiology --- Histological Techniques --- Human Anatomy & Physiology --- Health & Biological Sciences --- 577.2 --- Molecular bases of life. Molecular biology --- 577.2 Molecular bases of life. Molecular biology --- Bone marrow cells --- Platelets --- Thrombocytes --- Blood cells --- Hematology. --- Haematology --- Internal medicine --- Diseases --- Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cytologists --- Blood platelets - Laboratory manuals --- Megakaryocytes - Laboratory manuals

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Platelets, Fourth Edition, integrates the entire field of platelet biology, pathophysiology, and clinical medicine with contributions from 142 world experts from 18 countries. This award-winning reference provides clear presentations by basic scientists on the cellular, molecular, and genetic mechanisms of platelets and the role of platelets in thrombosis, hemorrhage, inflammation, antimicrobial host defense, wound healing, angiogenesis and cancer. It also provides start-of-the-art presentations by hematologists, cardiologists, stroke physicians, blood bankers, pathologists and other clinicians on platelet function testing, disorders of platelet numbers and function, antiplatelet therapy and therapy to increase platelet numbers and/or function. Since the publication of the Third Edition of Platelets, there has been a rapid expansion of knowledge in both basic biology of platelets and the clinical approach to platelet-related diseases. This Fourth Edition of Platelets draws all this information into a single, comprehensive and authoritative resource.

Blood platelets. --- Blood Platelets. --- Blood Platelet Disorders. --- Thrombocytopathy --- Blood Platelet Disorder --- Disorder, Blood Platelet --- Disorders, Blood Platelet --- Platelet Disorder, Blood --- Platelet Disorders, Blood --- Thrombocytopathies --- Platelets --- Thrombocytes --- Blood Platelet --- Platelet --- Platelet, Blood --- Platelets, Blood --- Thrombocyte --- Blood cells --- Megakaryocytes

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A rapid increase in our understanding of the biology of platelets and their role in disease in recent years has been paralleled by increasing successes with established platelet-modifying therapies in many clinical conditions. This text focuses on the clinical role of platelets in a wide variety of haematologic, cardiovascular and other disorders, providing a practical, clinically relevant handbook for all clinicians and researchers interested in platelets and their role in disease. Covering platelet physiology, bleeding disorders, thrombotic disorders and antithrombotic therapy. Chapters cover all the conventional and less conventional aspects of platelet involvement in disease, with an emphasis on recent clinical developments. Clear take home messages have been included in each chapter to aid clinical practice. With contributions from leading experts across three continents, Platelets in Hematologic and Cardiovascular Disorders is an up-to-date, well illustrated, practical resource for everyone involved in clinical practice with platelet disorders.

Blood platelets --- Blood --- Cardiovascular system --- Blood Platelets --- Cardiovascular diseases --- Clinical hematology --- Hematologic diseases --- Hematology --- Platelets --- Thrombocytes --- Blood cells --- Megakaryocytes --- Diseases --- pathology --- Blood platelets. --- Diseases. --- Hematologic Diseases --- Cardiovascular Diseases --- Blood Platelet Disorders --- physiopathology

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Blood Platelet Disorders. --- Blood Platelets --- Thrombosis --- Thrombocytopathy --- Blood Platelet Disorder --- Disorder, Blood Platelet --- Disorders, Blood Platelet --- Platelet Disorder, Blood --- Platelet Disorders, Blood --- Thrombocytopathies --- pathology. --- physiology. --- blood. --- Blood platelet disorders --- Blood platelets --- Blood Platelet Disorders --- Platelets --- Thrombocytes --- Blood cells --- Megakaryocytes --- Blood --- pathology --- physiology --- blood --- Diseases

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Are platelets cells? (Not everyone agrees, since they are non-nucleate). And if platelets are cells - which all specialists consider at the time being - are they immune cells? The issue that platelets participate in immunity is no longer debated; however, the issue that they are key cells in immunity is challenged. It has even been proposed a couple of years ago that platelets can present antigen to T-lymphocytes by using their HLA class I molecules. No one has the same functional definition of platelets. The ‘Frontiers Research Topic’- coordinators’ own view is that platelets are primarily repairing cells, what they do in deploying tools of physiological inflammation. This function is better acknowledged as primary hemostasis, i.e. platelet adherence to injured or wounded vessels, followed by activation, aggregation, and constitution of the initial clot. Platelets would thus repair damaged vascular endothelium; so doing, as they patrol to detect damages, they sense danger along the vascular arborescence. As the latter is immense, platelets get close to tissues, which are not allowed to them under ‘physiological’ conditions but are readily accessible in pathology. Platelets are equipped with a variety of Pathogen Recognition Receptors such as TLRs; they have a complete signalosome, which is functional until the phosphorylation of NFkB; they have been proved to retro-transcribe RNA and synthesize de novo proteins; etc. Platelets participate to inflammation along the whole spectrum: from physiological (tissue repair, healing) to acute/severe inflammation (as can be seen in e.g. sepsis). In general, platelets engage complex interactions with most infectious pathogens. We propose there to cover those topics - from physiology to pathology, that put platelets within cells that not only take place in-, but also are key players of-, innate immunity. The relation of platelets with adaptive immunity is even more complex. Not everyone is convinced that platelets present antigens; however, platelets influence adaptive immunity since they have mutual interactions with Dendritic cells, Monocytes/Macrophages, and B-lymphocytes (the key players of antigen presentation); they also have mutual interactions with T-lymphocytes, though is issue is less clearly deciphered. We propose to also cover these topics - or to present the forum. There is another issue which is medically relevant - speaking of physiology/physiopathology-: this is fetal maternal incompatibility of platelet specific antigens (the HPA system) and the likely formation of maternal antibodies that often injure the newborn with risks of severe thrombocytopenia and intracranial hemorrhage. We propose an update on this issue as well. Last, platelets are very special because they can be directly therapeutic (by transfusion), even when being offered by a generous blood donor displaying given genetic and phenotypic parameters to a patient/recipient in need, who also display his/her own genetic and phenotypic parameters, which - for a large part - differ from the donor's ones. Besides immunization - via mechanisms probably close to the fetal maternal platelet incompatibility, but likely not similar -, transfusion has allowed the identification of the tremendous capacity of platelets to mediate inflammation: we propose to conclude the Topics with this item/forum.

Blood platelets --- Immunopathology. --- Immunologic diseases. --- Immunology. --- Blood platelets. --- Infection. --- Immunity. --- Immune diseases --- Immune disorders --- Immune system --- Immunologic disorders --- Immunological diseases --- Diseases --- Clinical immunology --- Pathology --- Immunology --- Infectious diseases --- Medical microbiology --- Platelets --- Thrombocytes --- Blood cells --- Megakaryocytes --- Causes and theories of causation --- Infection --- Inflammation --- immunity --- platelets