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The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.

Cytological Techniques. --- Cells, Cultured. --- Sterilization --- Culture Media. --- 576.3 --- Celbiologie --- Celculturen --- Media, Culture --- Bacteriological Techniques --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- methods. --- methods --- Culture de cellule --- Protoplaste --- protoplasts --- Technique de culture --- Culture techniques --- Paramètre génétique --- genetic parameters --- Cell culture --- Cells, Cultured --- Culture Media --- Cytological Techniques --- Cultures (Biology) --- Cytology --- Technique --- culture media --- cells --- Cellules --- Laboratory manuals --- Culture --- Manuels de laboratoire

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Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

Cytology. --- Stem cells. --- Cell Biology. --- Stem Cells. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Colony-forming units (Cells) --- Mother cells --- Progenitor cells --- Mesenchymal Stem Cells --- Cell Culture Techniques. --- Cells, Cultured. --- Cytological Techniques. --- Multipotent Stem Cells --- Stromal Cells. --- cytology. --- Cell, Stromal --- Cells, Stromal --- Stromal Cell --- Cytologic Technics --- Cytological Technic --- Cytological Technics --- Cytological Technique --- Technic, Cytological --- Technics, Cytological --- Technique, Cytological --- Techniques, Cytological --- Cytologic Technic --- Technic, Cytologic --- Technics, Cytologic --- Cell Biology --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Cell Culture --- Cell Culture Technique --- Cell Cultures --- Culture Technique, Cell --- Culture Techniques, Cell --- Cells, Cultured --- methods --- Cytology

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Cell Differentiation --- Cell Transformation, Neoplastic --- Cells, Cultured --- Hematopoiesis --- Hematopoietic Stem Cells --- Leukemia --- Stem Cells --- cytology --- Cell Differentiation. --- Cell Transformation, Neoplastic. --- Cells, Cultured. --- Hematopoiesis. --- Leukemia. --- Blood formation --- Hemopoiesis --- Blood --- Leucocythaemia --- Leucocythemia --- Leucocythaemias --- Leucocythemias --- Leukemias --- Myeloproliferative Disorders --- Hematologic Neoplasms --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Haematopoiesis --- Hematopoiesis, Medullary --- Medullary Hematopoiesis --- Neoplastic Cell Transformation --- Transformation, Neoplastic Cell --- Tumorigenic Transformation --- Neoplastic Transformation, Cell --- Cell Neoplastic Transformation --- Cell Neoplastic Transformations --- Cell Transformations, Neoplastic --- Neoplastic Cell Transformations --- Neoplastic Transformations, Cell --- Transformation, Cell Neoplastic --- Transformation, Tumorigenic --- Transformations, Cell Neoplastic --- Transformations, Neoplastic Cell --- Transformations, Tumorigenic --- Tumorigenic Transformations --- Cell Line, Transformed --- Differentiation, Cell --- Cell Differentiations --- Differentiations, Cell --- Embryo, Mammalian --- Gene Expression Regulation --- Cell Lineage --- cytology.

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Cells. --- Cells, Cultured. --- Cytogenetics. --- Plants --- 576.32/.36 --- 57.086.83 --- 57.086.83 Methods and equipment for the cultivation of cells and tissues --- Methods and equipment for the cultivation of cells and tissues --- 576.32/.36 Cell physiology. Cell biochemistry. Cell biophysics. Molecular biology of the cell --- Cell physiology. Cell biochemistry. Cell biophysics. Molecular biology of the cell --- Cytogenetic --- Cytogenetic Analysis --- Chromosome Disorders --- Sex Chromosome Disorders --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Cell --- Cell Biology --- genetics. --- plants --- Cell culture --- cytogenetics --- Cell differentiation --- Meristems --- Préservation --- Preservation --- Freezing --- In vitro culture --- Micropropagation --- cells --- Cells --- Cells, Cultured --- Cytogenetics --- genetics --- Preservation. --- Plant tissue culture --- Cell culture of plants --- Cytodifferentiation in higher plants --- Cryopreservation of cultured plant cells --- Somatic cell genetics of plants

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Oncology. Neoplasms --- Gastroenterology --- Carcinogenesis --- Liver cells --- Cell transformation --- Cell culture --- Congresses --- Differentiation --- Carcinogens. --- Cell Differentiation. --- Cell Transformation, Neoplastic. --- Cells, Cultured. --- Liver Neoplasms, Experimental --- Liver --- -Cell culture --- -Cell transformation --- -Liver cells --- -#Lilly --- Hepatic cells --- Hepatocytes --- Cells --- Culture alteration (Cytology) --- Transformation of cells --- Tissue culture --- Cultures (Biology) --- Cytology --- Cancer --- Oncogenesis --- Pathogenesis of cancer --- Tumorigenesis --- Pathology --- Genetic toxicology --- Cultured Cells --- Cell, Cultured --- Cultured Cell --- Organ Culture Techniques --- Coculture Techniques --- Cell Culture Techniques --- Culture Techniques --- Human Umbilical Vein Endothelial Cells --- Neoplastic Cell Transformation --- Transformation, Neoplastic Cell --- Tumorigenic Transformation --- Neoplastic Transformation, Cell --- Cell Neoplastic Transformation --- Cell Neoplastic Transformations --- Cell Transformations, Neoplastic --- Neoplastic Cell Transformations --- Neoplastic Transformations, Cell --- Transformation, Cell Neoplastic --- Transformation, Tumorigenic --- Transformations, Cell Neoplastic --- Transformations, Neoplastic Cell --- Transformations, Tumorigenic --- Tumorigenic Transformations --- Cell Line, Transformed --- Differentiation, Cell --- Cell Differentiations --- Differentiations, Cell --- Embryo, Mammalian --- Gene Expression Regulation --- Cell Lineage --- Oncogens --- Tumor Initiators --- Tumor Promoters --- Initiators, Tumor --- Promoters, Tumor --- Benzidines --- chemically induced. --- cytology. --- -Congresses --- Technique --- Pathogenesis --- Carcinogens --- Cell Differentiation --- Cells, Cultured --- Cell Transformation, Neoplastic --- #Lilly --- Differentiation&delete& --- chemically induced --- cytology --- Carcinogen --- Oncogen --- Tumor Initiator --- Tumor Promoter --- Initiator, Tumor --- Promoter, Tumor --- Carcinogenesis - Congresses --- Liver cells - Differentiation - Congresses --- Cell transformation - Congresses --- Cell culture - Congresses