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Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new technique. The chapter on immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.


Flow cytometry --- Flow Cytometry --- Histology. Cytology --- Biological microscopy --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- methods --- Flow cytometry - Handbooks, manuals, etc. --- ophanging (autotechnologie) --- microscopie --- flowcytometrie --- moleculaire biologie

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Flow cytometry allows for the measurement of different aspects of a cell or particles in real time. Applications include quantifying various types of T cells to determine the presence of various immune disorders, such as whether HIV has progressed to AIDS, studying the dynamics of immune signaling in cells to detect the suppression of normal immune function, and analyzing biopsy specimens for tumors, to aid in cancer diagnosis and prognosis. In addition to simplifying the technique and using vocabulary accessible to those not trained in cell biology and immunology, the book includes the following 4 unqiue features: 1) Tips on how to set up an experiment for flow cytometry optimially 2) An expanded data set of flow staining including multiparameter flow cytometry 3) Detailed staining protocols for flow cytometry (ex. protocols optimized for transcription factors, secreted cytokines, phospho-antibodies, and surface antigens) 4) Section devoted to antibody development and conjugation.

Flow cytometry. --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Monoclonal antibodies. --- Medical laboratories. --- Antibodies. --- Laboratory Medicine. --- Diagnosis, Laboratory --- Health facilities --- Laboratories --- Antibodies, Monoclonal --- Monoclonal immunoglobulins --- Immunoglobulins --- Molecular cloning --- Laboratory medicine. --- Clinical medicine --- Clinical pathology --- Diagnostic laboratory tests --- Laboratory diagnosis --- Laboratory medicine --- Medical laboratory diagnosis --- Diagnosis --- Pathology --- Antibodies --- Immune globulins --- Immune serum globulin --- Blood proteins --- Globulins --- Plasma cells --- Antibody diversity --- Antigens --- Bacterial immunoglobulin-binding proteins

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This volume highlights the most interesting biomedical and clinical applications of high-dimensional flow and mass cytometry. It reviews current practical approaches used to perform high-dimensional experiments and addresses key bioinformatic techniques for the analysis of data sets involving dozens of parameters in millions of single cells. Topics include single cell cancer biology; studies of the human immunome; exploration of immunological cell types such as CD8+ T cells; decipherment of signaling processes of cancer; mass-tag cellular barcoding; analysis of protein interactions by proximity ligation assays; Cytobank, a platform for the analysis of cytometry data; computational analysis of high-dimensional flow cytometric data; computational deconvolution approaches for the description of intracellular signaling dynamics; and hyperspectral cytometry. All 10 chapters of this book have been written by respected experts in their fields. It is  an invaluable reference book for both basic and clinical researchers.

Flow cytometry. --- Bioinformatics. --- Computational biology. --- Medicine. --- Cancer research. --- Immunology. --- Biology --- Biomedicine. --- Cancer Research. --- Biological Techniques. --- Bio-informatics --- Biological informatics --- Information science --- Computational biology --- Systems biology --- Immunobiology --- Life sciences --- Serology --- Cancer research --- Clinical sciences --- Medical profession --- Human biology --- Medical sciences --- Pathology --- Physicians --- Technique. --- Data processing --- Bioinformatics --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Oncology. --- Cytology --- Research --- Methodology. --- Cell biology --- Cellular biology --- Cells --- Cytologists --- Tumors --- Biology—Technique. --- Cancer. --- Cancer Biology. --- Cancers --- Carcinoma --- Malignancy (Cancer) --- Malignant tumors

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This book highlights the current state of the art in single cell analysis, an area that involves many fields of science – from clinical hematology, functional analysis and drug screening, to platelet and microparticle analysis, marine biology and fundamental cancer research. This book brings together an eclectic group of current applications, all of which have a significant impact on our current state of knowledge. The authors of these chapters are all pioneering researchers in the field of single cell analysis. The book will not only appeal to those readers more focused on clinical applications, but also those interested in highly technical aspects of the technologies. All of the technologies identified utilize unique applications of photon detection systems.

Engineering. --- Cancer research. --- Immunology. --- Cell biology. --- Biomedical engineering. --- Biomedical Engineering. --- Biological and Medical Physics, Biophysics. --- Cancer Research. --- Cell Biology. --- Flow cytometry. --- Cytophotometry. --- Cytology --- Photometry --- Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Technique --- Oncology. --- Cytology. --- Biomedical Engineering and Bioengineering. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists --- Tumors --- Immunobiology --- Life sciences --- Serology --- Clinical engineering --- Medical engineering --- Bioengineering --- Biophysics --- Engineering --- Medicine --- Biophysics. --- Biological physics. --- Biological physics --- Medical sciences --- Physics --- Cancer research

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Flow cytometry --- Laboratory manuals --- 57.086.8 --- 576.3 --- -Cell counting, Laser --- Cell separation, Laser --- Cell sorting, Laser --- Flow cytofluorometry --- Flow cytophotometry --- Laser cell counting --- Laser cell separation --- Laser cell sorting --- Cytometry --- Methods and equipment for studying living cells and tissues --- General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- -Methods and equipment for studying living cells and tissues --- 57.086.8 Methods and equipment for studying living cells and tissues --- 576.3 General cytology. The cell as a biological system. General plan, chemical composition, physicochemical and molecular properties --- -57.086.8 Methods and equipment for studying living cells and tissues --- Cell counting, Laser --- Flow cytometry - Laboratory manuals