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The emergence of bacterial strains which are resistant to current treatments is a concern. To find an alternative, avoiding cross resistances, “new” targets have to be defined. 3’, 6-dinonyl neamine, an amphiphilic aminoglycoside derivative targeting the bacterial membrane has shown its efficiency and low toxicity (Zimmermann et al. 2013). In this study, we first investigated the interaction of this molecule with lipoteichoic acid (LTA) (fluorescence of bodipy –TR cadaverine), an essential component of Gram positive bacteria cell wall. Then we studied the consequences of this interaction on the bacterial membrane, and two parameters interested us: the potential and the permeability of the membrane. We have shown a modification of membrane potential (fluorescence of DISC3 (5) and membrane permeabilization (fluorescence of propidium iodide) following the interaction with LTA. We finally observed a modification of the morphology of bacillus ( B subtilis) when exposed to 3’, 6-dinonyl neamine (increase length). These first results allow us to better understand the way this neamine derivative works and this could be a starting point for further studies, to deeper investigate the effects on Gram positive bacteria membrane. L'émergence de souches bactériennes résistantes aux traitements antibiotiques actuels est préoccupante. Dans le but de trouver une alternative évitant les résistances croisées, de « nouvelles » cibles doivent être définies. Le 3',6-dinonyl néamine, un dérivé amphiphile d'aminoglycoside ciblant la membrane bactérienne, a démontré son efficacité et sa faible toxicité (Zimmermann et al, 2013). Dans cette étude, nous nous sommes intéressés, dans un premier temps, à l'interaction de cette molécule avec l'acide lipoteichoïque (LTA) (fluorescence de la Bodipy-TR-Cadaverine), un composant essentiel de la paroi des bactéries Gram positif. Par la suite, nous avons étudié les conséquences, sur la membrane bactérienne, de cette interaction, en nous intéressant à deux paramètres : le potentiel de membrane et la perméabilité membranaire. Nous avons pu démontrer que, suite à leur interaction avec le LTA, ces dérivés induisaient une modification du potentiel de membrane (fluorescence du DiSC3(5)) et une perméabilisation membranaire (fluorescence de l'iodure de propidium). Nous avons finalement observé une modification de la morphologie de bacilles (B.subtilis) exposés au 3',6-dinonyl néamine (augmentation de la longueur). Ces premiers résultats nous permettent de mieux comprendre le mode d'action de ce dérivé de la néamine et pourraient constituer un point de départ pour la réalisation d'études complémentaires visant à approfondir les effets membranaires (modification du profil lipidique de la membrane) sur les bactéries Gram positif.
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This book provides in-depth insights into the diversity of actinobacteria, their various medical aspects, and their biotechnological applications. It is aimed at graduate students and researchers working with actinobacteria who need to know about recent advances in their research fields. The book, which is divided into four sections, contains 13 chapters that review the most recent methods, discuss various applications of actinobacteria, introduce some interesting results, and explore future prospects for the topics covered.
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Actinobacteria. --- Actinomycetes --- Gram-positive bacteria --- Actinomicetals
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GC Rich Sequence --- Genomics. --- Gram-Positive Bacteria --- Gram-positive bacteria. --- Microbial genetics. --- Genetics. --- Genetics.
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GRAM-POSITIVE BACTERIA --- MILK --- ANTIMICROBIAL AGENTS --- GRAM-POSITIVE BACTERIA --- MILK --- ANTIMICROBIAL AGENTS
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Slurry --- Slurry --- Aromatic compounds --- Aromatic compounds --- Biodegradation. --- Biodegradation --- Anaerobiosis --- Anaerobiosis --- gram positive bacteria --- gram positive bacteria --- culture media --- culture media
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essential oils --- essential oils --- Antimicrobial properties --- Antimicrobial properties --- gram positive bacteria --- gram positive bacteria --- foodborne diseases --- foodborne diseases
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Slurry --- Slurry --- Biodegradation. --- Biodegradation --- Anaerobiosis --- Anaerobiosis --- Phenolic compounds --- Phenolic compounds --- gram positive bacteria --- gram positive bacteria --- Fermentation --- Fermentation
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Gram-Positive Bacteria. --- Bacterial Vaccines. --- Drug Resistance, Microbial. --- Gram-Positive Bacterial Infections. --- Gram-positive bacterial infections. --- Gram-positive bacteria. --- Infections à bactéries Gram positives --- Bactéries Gram positives --- Gram-positive bacteria --- Drug resistance, bacterial --- ultrastructure --- classification
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Smart Bioremediation Technologies: Microbial Enzymes provides insights into the complex behavior of enzymes and identifies metabolites and their degradation pathways. It will help readers work towards solutions for sustainable medicine and environmental pollution. The book highlights the microbial enzymes that have replaced many plant and animal enzymes, also presenting their applications in varying industries, including pharmaceuticals, genetic engineering, biofuels, diagnostics and therapy. In addition, new methods, including genomics and metagenomics, are being employed for the discovery of new enzymes from microbes. This book brings all of these topics together, representing the first resource on how to solve problems in bioremediation. --
Bioremediation. --- Microbial enzymes. --- Actinobacteria. --- Biodegradation, Environmental. --- Bacteria --- enzymology. --- Natural Attenuation, Pollution --- Bioremediation --- Phytoremediation --- Environmental Biodegradation --- Pollution Natural Attenuation --- High G+C Gram-Positive Bacteria --- Actinomycetes --- Gram-Positive Bacteria, High G+C --- Enzymes --- Microbiological chemistry --- Environmental biotechnology --- Biodegradation --- Pollution --- Actinomycete
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