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The ideal practical introduction to using PCR (polymerase chain reaction) techniques in the laboratory. The book enables a novice to understand the techniques described, how to use them and how to manipulate them if necessary. A concise information source for use both within the lab and out, providing basic theory, background material, and suggestions for suitable protocols.

Polymerase chain reaction

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Polymerase chain reaction --- Methodology.

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This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. Topics covered include: principles of PCR, fluorescent chemistries, instrumentation, quantification strategies, extraction and purification of nucleic acids, sample preparation, controls for validation, primers and probes, standardization of methods, MIQE guidelines, mRNA expression and PCR arrays. This book provides a comprehensive overview of PCR theory, instrumentation and methods. The book represents an excellent, detailed guide for anyone interested in the development and use of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.

Polymerase chain reaction.

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The invention of the polymerase chain reaction (PCR) won the Nobel Prize in Chemistry in 1994 and remains one of the most important scientific discoveries of the twentieth century. More than 50,000 researchers in the United States use PCR replication technology, yet there is a significant absence of any recent publications on this subject. In this book, Dr Stephen A. Bustin, a world-renowned PCR expert, examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research. The book contains personal reflections, opinions, and comments by leading authorities on the many applications of the PCR and how this technology has revolutionised their respective areas of interest. Bustin explains the ways in which PCR has overcome many obstacles in life science and clinical research and charts the PCR's development from time-consuming, low throughput, non-quantitative procedure to today's rapid, high throughput, quantitative super method.

Polymerase chain reaction --- Polymerase chain reaction. --- Diagnostic use.

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This publication by the World Health Organization outlines the establishment of a laboratory network and a new external quality assessment program for PCR-based diagnosis of Buruli ulcer in the African Region. It addresses the need for improved diagnostic accuracy in endemic countries following the cessation of a previous assessment program by the Institute of Tropical Medicine in Antwerp. The document aims to harmonize standard operating procedures, enhance laboratory performance, and ensure reliable data for WHO. It targets healthcare professionals and laboratory technicians involved in disease diagnosis and management.

Polymerase chain reaction. --- Quality control. --- Polymerase chain reaction --- Quality control