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As an important tool of systems biology, proteomics has enabled a deep understanding of different plant processes and functions. Complemented with genomic data, computational tools, and improved sample preparation strategies, proteomics has an unprecedented opportunity to characterize plant proteoforms in high spatial and temporal resolution. This special issue of Plant Proteomic Research 4.0 captures the recent advancements in proteomics and addresses the current challenges of plant stress response and resilience in the ever-changing climate. It contains 12 articles, including three reviews and nine original research articles. The three reviews deal with pollen phosphoproteomics, starch biosynthesis-related proteins and posttranslational modifications (PTMs) in rice developing seeds, and PTMs of waxy proteins in rice grain. The nine research articles include three related to temperature, two on water stress, two on salt stress, one on fungal pathogen, and the last one on field-grown potato apoplast proteome. The articles reflect the current frontiers of plant proteomics, focusing on themes of environmental stresses, proteoforms/PTMs, crop species, and new development in data-independent acquisition mass spectrometry. They provide readers insights into current technologies, their utility in understanding plant growth and resilience, as well as directions of proteomics in the frontiers of systems biology and synthetic biology.

Proteomics.

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As an important tool of systems biology, proteomics has enabled a deep understanding of different plant processes and functions. Complemented with genomic data, computational tools, and improved sample preparation strategies, proteomics has an unprecedented opportunity to characterize plant proteoforms in high spatial and temporal resolution. This special issue of Plant Proteomic Research 4.0 captures the recent advancements in proteomics and addresses the current challenges of plant stress response and resilience in the ever-changing climate. It contains 12 articles, including three reviews and nine original research articles. The three reviews deal with pollen phosphoproteomics, starch biosynthesis-related proteins and posttranslational modifications (PTMs) in rice developing seeds, and PTMs of waxy proteins in rice grain. The nine research articles include three related to temperature, two on water stress, two on salt stress, one on fungal pathogen, and the last one on field-grown potato apoplast proteome. The articles reflect the current frontiers of plant proteomics, focusing on themes of environmental stresses, proteoforms/PTMs, crop species, and new development in data-independent acquisition mass spectrometry. They provide readers insights into current technologies, their utility in understanding plant growth and resilience, as well as directions of proteomics in the frontiers of systems biology and synthetic biology.

Proteomics.

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This volume is divided in six section covering the most experimental approaches involved in the study of the unfolded protein response (UPR) pathway. Chapters detail determination of unfolded protein levels, methods to study UPR signal transmission, analysing the outcomes of the UPR pathway activation, UPR studies in mammalian models, UPR in alternative models, and UPR and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, The Unfolded Protein Response: Methods and Protocols aims to describe key methods and approaches used in the study of the UPR pathway and its complex cellular implications. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics

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Proteins. --- Proteins --- Analysis. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics

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This detailed volume explores techniques in clinical proteomics, an emerging discipline aimed at deciphering the molecular mechanisms underlying the progression of diseases and at identifying new biomarkers and therapeutic targets to expand the physicians’ toolbox for precision medicine-based patient care. From sample processing to multi-omics approaches, the book provides straight-forward protocols on a wide array of vital areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Clinical Proteomics: Methods and Protocols serves as an ideal guide for researchers working to expand upon the knowledge base needed to push forward toward a more personalized version of medicine.

Proteins. --- Medicine. --- Clinical Medicine. --- Health Workforce --- Proteids --- Biomolecules --- Polypeptides --- Proteomics