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This volume provides updated protocols for chemical protein synthesis. Chapters guide readers through development methods, strategies, and applications of protein chemical synthesis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chemical Protein Synthesis aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. .
Proteins. --- Biochemistry. --- Biosynthesis. --- Biological Chemistry. --- Biological synthesis --- Synthesis, Biological --- Biochemical engineering --- Biochemistry --- Organic compounds --- Synthetic biology --- Biochemical templates --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Synthesis --- Composition
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This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchers including graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs. .
Biochemistry. --- Proteins. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. Chapters 3, 4, 8, and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Biochemistry. --- Biology—Technique. --- Biological Techniques. --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Composition
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Proteomics. --- Biochemistry. --- Chemistry, Organic. --- Organic chemistry --- Chemistry --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Molecular biology --- Proteins --- Composition
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This second edition of Medical Biochemistry is supported by more than 45 years of teaching experience, providing coverage of basic biochemical topics, including the structural, physical, and chemical properties of water, carbohydrates, lipids, proteins, and nucleic acids. In addition, the general aspects of thermodynamics, enzymes, bioenergetics, and metabolism are presented in straightforward and easy-to-comprehend language. This book ties these concepts into more complex aspects of biochemistry using a systems approach, dedicating chapters to the integral study of biological phenomena, including cell membrane structure and function, gene expression and regulation, protein synthesis and post-translational modifications, metabolism in specific organs and tissues, autophagy, cell receptors, signal transduction pathways, biochemical bases of endocrinology, immunity, vitamins and minerals, and hemostasis.
Clinical biochemistry. --- Biochemistry --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Medical sciences --- Medical biochemistry --- Pathobiochemistry --- Pathological biochemistry --- Pathology --- Composition --- Biochemistry. --- Biochemical Phenomena
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Tellurium, a well-known chalcogen, finds potential applications in various fields from chemistry to other branches of science such as nanotechnology and macromolecular science. However, its safety must also be taken into consideration when exploring its industrial applications. This book explores the breadth of tellurium‘s applications, outlines strategies for industrial use, and describes the safety concerns of this element.
Inorganic Chemistry. --- Safety. --- Tellurium. --- Toxicology. --- Biochemistry. --- Chemistry, Organic. --- Chalcogens --- Nonmetals --- Organic chemistry --- Chemistry --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Medical sciences --- Composition
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This volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Glycosylation: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field. .
Biotechnology. --- Biochemistry. --- Bioinformatics. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Chemical engineering --- Genetic engineering --- Data processing --- Composition
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This book presents an authoritative review of analytical methods used for diagnostics, medical therapy and for forensic purposes. Divided into 4 parts, the book discusses new challenges in bioanalytics, covers bioanalysis as a source of clinical, pharmaceutical and forensic information, explores natural resources as a source of biologically active compounds, and offers new analytical strategies and equipment solutions. Written by interdisciplinary expert academics, this work will appeal to a wide readership of students, researchers and professionals interested in the fields of medicine, chemistry, pharmaceutical, life and health sciences, engineering and environmental protection. Clinicians and employees of forensic laboratories will also find this work instructive and informative.
Analytical biochemistry. --- Analytic biochemistry --- Bioanalytic chemistry --- Bioanalytical chemistry --- Analytical chemistry --- Biochemistry --- Analytical chemistry. --- Biochemistry. --- Biophysics. --- Molecular probes. --- Clinical medicine --- Analytical Chemistry. --- Analytical Biochemistry. --- Biological Chemistry. --- Bioanalysis and Bioimaging. --- Biological Sensors and Probes. --- Clinical Research. --- Research. --- Bioprobes --- Probes, Molecular --- Molecular biology --- Affinity labeling --- Biological physics --- Biology --- Medical sciences --- Physics --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Analysis, Chemical --- Analytic chemistry --- Chemical analysis --- Chemistry, Analytic --- Technique --- Composition
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Cation Transporters in Plants presents expert information on the major cation transporters, along with developments of various new strategies to cope with the adverse effects of abiotic and biotic stresses. The book will serve as a very important repository for the scientist, researcher, academician and industrialist to enhance their knowledge about cation transport in plants. Further, applications listed in the book will facilitate future developments in crop designing strategies. This comprehensive resource provides an alternative strategy for abiotic and biotic stress management in agricultural and horticultural crops. In addition, it will further improve basic knowledge om the origin and mechanism of cation homeostasis and their role in developmental transition and stress regulation.
Biological transport. --- Plants. --- Biochemistry. --- Biology --- Chemistry --- Medical sciences --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Botany --- Flora --- Plant kingdom --- Plantae --- Vascular plants --- Vegetable kingdom --- Vegetation --- Wildlife --- Diffusion --- Osmosis --- Membrane transport --- Passive transport, Biological --- Physiological transport --- Transport, Biological --- Composition --- Plants --- Plant translocation. --- Botanical chemistry. --- Cations. --- Metabolism.
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This detailed volume covers diverse aspects of P2X7 receptor analysis, ranging from its molecular structure to related pharmacological and immunological tools, via its analysis in heterologous expression systems as well as assays using primary cells and whole animal models. After three introductory chapters that focus on its structure, ligands, and physiological functions, the book details the generation of antibody and nanobody tools for P2X7 receptors, provides protocols for the analysis of expressed P2X7 receptors with a focus on their electrophysiological analysis, as well as protocols for the investigation of P2X7 down-stream signaling in immune cells by flow cytometry. Mouse models and procedures suited to investigate P2X7-mediated effects in other primary cells and in vivo are also explained. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The P2X7 Receptor: Methods and Protocols is a valuable reference not only for the growing community fascinated by this unusual ion channel but also for a broad readership interested in ion channels or purinergic receptors.
Biochemistry. --- Immune system. --- Cellular signal transduction. --- Pharmacology. --- Immune Cell Signalling. --- Drug effects --- Medical pharmacology --- Medical sciences --- Chemicals --- Chemotherapy --- Drugs --- Pharmacy --- Cellular information transduction --- Information transduction, Cellular --- Signal transduction, Cellular --- Bioenergetics --- Cellular control mechanisms --- Information theory in biology --- Immunological system --- Anatomy --- Immunology --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Biology --- Chemistry --- Physiological effect --- Composition
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