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Recently, stem cells have been drawing increasing interest in basic and translational research that aims to understand stem cell biology and generate new therapies for various disorders. Many stem cells can be cultured in 2D relatively easily using tissue culture plastic. However, many of these cultures do not represent the natural conditions of stem cells in the body. In the body, microenvironments include numerous supporting cells and molecules. Therefore, researchers and clinicians have sought ideal stem cell preparations for basic research and clinical applications, which may be attainable through 3D culture of stem cells. The 3D cultures mimic the conditions of the natural environment of stem cells better, as cells in 3D cultures exhibit many unique and desirable characteristics that could be beneficial for therapeutic interventions. 3D stem cell cultures may employ supporting structures, such as various matrices or scaffolds, in addition to stem cells, to support complex structures. This book brings together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cell culture techniques and also to highlight stem cell preparations for possible novel therapeutic applications.

Research & information: general --- Biology, life sciences --- hematopoiesis --- hematopoietic stem cells --- stem cell culture --- 2D culture --- 3D culture --- embryonic stem cells --- three-dimensional --- self-assembling scaffold --- pluripotency --- culture conditions --- expansion --- growth --- niche --- human cortical progenitors --- silicon pillars --- cell growth --- hiPSC-derived neural progenitors --- cerebral cortex --- carcinogen --- protein phosphatase 2A (PP2A) --- intestinal tumor --- intestinal organoid --- Lgr5+ crypt stem cell --- mouse embryonic stem cell --- differentiation protocol --- ureteric bud progenitor cells --- 3D kidney organoids --- intestinal organoids --- canine intestine --- differentiation --- organoid culture --- induced pluripotent stem cells --- neurospheres --- neurite outgrowth --- neurotoxicity --- hBM-MSCs --- cytokines --- tenogenic markers --- cyclic strain --- 3D microenvironment --- PLGA carriers --- bioreactor --- cardiac microtissues --- iPSC-derived cardiomyocytes --- cardiac fibroblasts --- cardiac fibrosis --- cardiac rhythm --- TGF-β signalling --- drug screening --- in vitro model --- stem cell --- 3D --- culture condition --- regenerative medicine --- scaffold --- organoid --- adipose tissue-derived mesenchymal stem cells --- stromal vascular fraction --- platelet rich plasma --- platelet concentrates --- veterinary regenerative medicine --- hematopoiesis --- hematopoietic stem cells --- stem cell culture --- 2D culture --- 3D culture --- embryonic stem cells --- three-dimensional --- self-assembling scaffold --- pluripotency --- culture conditions --- expansion --- growth --- niche --- human cortical progenitors --- silicon pillars --- cell growth --- hiPSC-derived neural progenitors --- cerebral cortex --- carcinogen --- protein phosphatase 2A (PP2A) --- intestinal tumor --- intestinal organoid --- Lgr5+ crypt stem cell --- mouse embryonic stem cell --- differentiation protocol --- ureteric bud progenitor cells --- 3D kidney organoids --- intestinal organoids --- canine intestine --- differentiation --- organoid culture --- induced pluripotent stem cells --- neurospheres --- neurite outgrowth --- neurotoxicity --- hBM-MSCs --- cytokines --- tenogenic markers --- cyclic strain --- 3D microenvironment --- PLGA carriers --- bioreactor --- cardiac microtissues --- iPSC-derived cardiomyocytes --- cardiac fibroblasts --- cardiac fibrosis --- cardiac rhythm --- TGF-β signalling --- drug screening --- in vitro model --- stem cell --- 3D --- culture condition --- regenerative medicine --- scaffold --- organoid --- adipose tissue-derived mesenchymal stem cells --- stromal vascular fraction --- platelet rich plasma --- platelet concentrates --- veterinary regenerative medicine