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This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Bioenginyeria --- Biologia computacional --- Biologia --- Biologia de sistemes --- Neurociència computacional --- Bioinformàtica --- Enginyeria biològica --- Enginyeria --- Enginyeria biomèdica --- Biònica --- Biotelemetria --- Enginyeria agronòmica --- Guerra biològica --- Medical genetics. --- Biotechnology. --- Biology—Technique. --- Medical Genetics. --- Biological Techniques. --- Chemical engineering --- Genetic engineering --- Clinical genetics --- Diseases --- Heredity of disease --- Human genetics --- Medical sciences --- Pathology --- Genetic disorders --- Genetic aspects --- Genètica mèdica --- Genètica humana --- Patologia --- Farmacogenètica --- Genètica veterinària --- Toxicologia genètica --- Consell genètic --- Cribratge genètic --- Malalties hereditàries

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Computational intelligence. --- Engineering --- Data processing. --- Intelligence, Computational --- Artificial intelligence --- Soft computing --- Cibernètica --- Seguretat informàtica --- Protecció de dades --- Protecció de dades personals --- Protecció de la informació (Informàtica) --- Regulació de dades --- Processament de dades --- Xifratge (Informàtica) --- Delegats de protecció de dades --- Dret a l'oblit --- Identitat digital --- Informàtica (Seguretat) --- Mesures de seguretat (Informàtica) --- Seguretat (Informàtica) --- Seguretat dels sistemes informàtics --- Sistemes de seguretat --- Control d'accés als ordinadors --- Seguretat de les xarxes d'ordinadors --- Pirates informàtics --- Automàtica --- Cervells electrònics --- Comunicació --- Electrònica --- Anàlisi de sistemes --- Biònica --- Control automàtic --- Ordinadors --- Robòtica --- Sistemes autoorganitzatius --- Sistemes de control biològic --- Teoria de la informació --- Xarxes neuronals (Informàtica)

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This open access book is only an introduction to show that radiation and radioisotopes (RI) are premier tools to study living plant physiology which leads to new findings. Who had ever imagined that we could see water in a plant? Who had ever imagined that we could see ions moving toward roots in solution? Who had ever imagined that we could see invisible gas (CO2) fixation and movement in a plant? These studies demonstrated for the first time that water, ions and gas can be visualized in living plants, which could be hardly seen by anyone before. This publication summarizes the results obtained by Nakanishi’s lab in The Univ. of Tokyo, based on her original concept and her original tools or systems. It is useful for professional scientists, plant physiologist, and those studying plant imaging. The chapters demonstrates the innovative imaging work of the author, using radioactive tracers and neutron beam to follow the absorption and transport manner of water as well as major, minor, and trace elements in plants. Through these studies the author developed a real-time macroscopic and microscopic imaging system able to apply commercially available gamma- and beta-ray emitters. The real-time movement of the elements is now possible by using 14C, 18F, 22Na, 28Mg, 32P, 33P, 35S, 42K, 45Ca, 48V, 54Mn, 55Fe, 59Fe, 65Zn, 86Rb, 109Cd, and 137Cs. The imaging methods was applied to study the effect of 137Cs following 3/11 Fukushima Daiichi nuclear plant accident, which has revealed the movements of radiocesium in the contaminated sites. .

Plant physiology. --- Biophysics. --- Biological physics. --- Radiology. --- Nuclear chemistry. --- Spectroscopy. --- Materials science. --- Plant Physiology. --- Biological and Medical Physics, Biophysics. --- Imaging / Radiology. --- Nuclear Chemistry. --- Spectroscopy/Spectrometry. --- Materials Science, general. --- Material science --- Physical sciences --- Analysis, Spectrum --- Spectra --- Spectrochemical analysis --- Spectrochemistry --- Spectrometry --- Spectroscopy --- Analytical chemistry --- Interferometry --- Optics --- Radiation --- Wave-motion, Theory of --- Absorption spectra --- Light --- Spectroscope --- Chemistry, Nuclear --- Chemistry, Physical and theoretical --- Radiological physics --- Physics --- Biological physics --- Biology --- Medical sciences --- Botany --- Plants --- Physiology --- Qualitative --- Plant Physiology --- Biological and Medical Physics, Biophysics --- Imaging / Radiology --- Nuclear Chemistry --- Spectroscopy/Spectrometry --- Materials Science, general --- Bioanalysis and Bioimaging --- Radiology --- Imaging Techniques --- Open Access --- Living plant activity --- Water imaging --- Element movement --- Visualization of gas fixation --- Fixed carbon movement --- Neutron beam imaging --- Radioisotope imaging --- RI --- 32P --- 33P --- Botany & plant sciences --- Biophysics --- Medical physics --- Medical imaging --- Nuclear chemistry, photochemistry & radiation --- Spectrum analysis, spectrochemistry, mass spectrometry --- Materials science --- Fisiologia vegetal --- Botànica --- Absorció de l'aigua en les plantes --- Assimilació de les plantes --- Creixement (Plantes) --- Ecofisiologia vegetal --- Fecundació de les plantes --- Fisiologia postcollita --- Ritmes biològics en les plantes --- Floració --- Germinació --- Metabolisme de les plantes --- Moviments de les plantes --- Nutrició vegetal --- Reproducció de les plantes --- Respiració de les plantes --- Saprofitisme --- Tropisme --- Biologia molecular vegetal --- Control --- Biofísica --- Química nuclear --- Espectroscòpia --- Anàlisi d'espectre --- Anàlisi dels espectres --- Anàlisi espectral --- Anàlisi espectroscòpica --- Anàlisi espectroquímica --- Espectres (Física) --- Espectroanàlisi --- Espectrografia --- Espectrometria --- Espectroquímica --- Astrofísica --- Interferometria --- Òptica --- Química --- Química analítica qualitativa --- Radiació --- Teoria del moviment ondulatori --- Diagrames de Grotrian --- Espectre ultraviolat --- Espectres atòmics --- Espectrometria de raigs alfa --- Espectrometria de raigs beta --- Espectroscòpia atòmica --- Espectroscòpia d'alta resolució --- Espectroscòpia d'electrons --- Espectroscòpia de làser --- Espectroscòpia de luminiscència --- Espectroscòpia de mobilitat d'ions --- Espectroscòpia de plasma --- Espectroscòpia molecular --- Espectroscòpia nuclear --- Espectroscòpia Raman --- Espectroscòpia de ressonància paramagnètica electrònica --- Radiació ultraviolada --- Ressonància magnètica nuclear --- Astronomia --- Espectre solar --- Espectres d'absorció --- Espectroscopis --- Llum --- Química física --- Biologia física --- Física biològica --- Biologia --- Física --- Absorció (Fisiologia) --- Biologia molecular --- Biònica --- Biomagnetisme --- Biomecànica --- Enginyeria biomèdica --- Física mèdica --- Interfícies biològiques --- Reologia (Biologia) --- Spectrum analysis.