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This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact

flow cytometry --- primary immunodeficency --- immunophenotyping --- diagnosis --- functional studies

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La cytométrie en flux, technique permettant l'analyse rapide des cellules, a considérablement évolué ces dernières décennies et a contribué à de nombreuses avancées scientifiques et médicales. Ses avancées ont ouvert la porte à des applications devenues incontournables dans différentes spécialités telles que l'hématologie, l'immunologie, la microbiologie, la biologie végétale et marine. La transversalité interdisciplinaire de la cytométrie en flux en fait toute la richesse. La deuxième édition de "La cytométrie en flux" fait le point, en 29 chapitres, sur toutes les nouveautés dues aux avancées instrumentales, à la miniaturisation des lasers, aux progrès de l'optique, de la fluidique mais aussi de l'informatique, des logiciels embarqués et de l'analyse de données, sans oublier les réactifs couvrant maintenant une gamme inimaginable il y a seulement encore quelques années. De nombreux thèmes sont ainsi abordés : Principes, techniques, statistiques appliquées à la cytométrie- Applications dans différents domaines (hématologie, immunologie, détection des microparticules, étude des cellules souches, pharmacologie anticancéreuse...)- Criblage à haut débit (high throughput screening), applications du fluorescent cell barcoding- Assurance qualité, accréditation- Avancées de la cytométrie couplée à d'autres techniques comme l'analyse spectrale et la spectrométrie de masse- Gestion des plateformes- Hygiène et sécurité. Les nombreuses illustrations en couleurs et les protocoles d'analyse viennent compléter ce livre didactique. Cet ouvrage est destiné aux enseignants et étudiants, chercheurs, biologistes, cliniciens, ingénieurs, techniciens et industriels désireux d'approfondir ou de mettre à jour leurs connaissances. Il permettra surtout aux utilisateurs non experts d'acquérir les concepts de base de la cytométrie en flux.

Flow Cytometry. --- Cytology --- Cytological Techniques --- Cytométrie de flux. --- Cytopathologie --- Cytologie --- Technique. --- methods. --- Technique

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The cooperation of highly specialized cell types maintains the homeostasis of multicellular organisms. The disturbance of that harmony contributes to the development of several diseases. Most of the cellular functions are executed by proteins, so it is essential to investigate biological processes at the protein level. Antibodies, complex biomolecules with high specificity, are used to recognize our protein of interest in a process known as “immunophenotyping”. One of the routinely used methods to study cellular proteins is flow cytometry, which detects cell surface or intracellular proteins at single-cell resolution. The other most frequent technique is the traditional immunohistochemical investigation of microscopic sections of human tissues. We called authors to publish their latest data studying cancer or autoimmune diseases by immunophenotyping.

Medicine --- CD8+CD28− T cells --- cancer immunology --- glioblastoma --- immunotherapy --- malignant glioma --- cancer --- accidental cell death --- oncosis --- DDR --- parthanatos --- flow cytometry --- systemic lupus erythematosus --- T cells --- glycosylation --- sialylation --- lectin binding --- glycosylation enzymes --- galectin 1 --- choriocarcinoma --- hydatidiform mole --- galectin --- gestational trophoblastic disease --- placental-specific gene --- systems biology --- trophoblast differentiation --- B cells --- non-switched B cells --- systemic sclerosis --- dcSSc --- TLR --- CD180 --- RP105 --- CpG --- IL-6 --- IL-10 --- natural autoantibodies --- IgM --- citrate synthase --- DNA topoisomerase I --- unfolded protein response --- Inositol-requiring enzyme 1 (IRE1) --- PKR-like endoplasmic reticulum kinase (PERK) --- Glucose-regulated protein 78 (GRP78) --- Activating transcription factor 6 (ATF6) --- immune cells --- T cell --- macrophage --- tumor microenvironment --- single cell mass cytometry --- metastatic breast cancer --- myeloid-derived suppressor cells --- immunophenotyping --- breast cancer --- trastuzumab --- chimeric antigen receptor --- cell therapy --- neuroendocrine neoplasia --- neuroendocrine tumor --- neuroendocrine carcinoma --- immunohistochemistry --- syntaxin 1 --- CD8+CD28− T cells --- cancer immunology --- glioblastoma --- immunotherapy --- malignant glioma --- cancer --- accidental cell death --- oncosis --- DDR --- parthanatos --- flow cytometry --- systemic lupus erythematosus --- T cells --- glycosylation --- sialylation --- lectin binding --- glycosylation enzymes --- galectin 1 --- choriocarcinoma --- hydatidiform mole --- galectin --- gestational trophoblastic disease --- placental-specific gene --- systems biology --- trophoblast differentiation --- B cells --- non-switched B cells --- systemic sclerosis --- dcSSc --- TLR --- CD180 --- RP105 --- CpG --- IL-6 --- IL-10 --- natural autoantibodies --- IgM --- citrate synthase --- DNA topoisomerase I --- unfolded protein response --- Inositol-requiring enzyme 1 (IRE1) --- PKR-like endoplasmic reticulum kinase (PERK) --- Glucose-regulated protein 78 (GRP78) --- Activating transcription factor 6 (ATF6) --- immune cells --- T cell --- macrophage --- tumor microenvironment --- single cell mass cytometry --- metastatic breast cancer --- myeloid-derived suppressor cells --- immunophenotyping --- breast cancer --- trastuzumab --- chimeric antigen receptor --- cell therapy --- neuroendocrine neoplasia --- neuroendocrine tumor --- neuroendocrine carcinoma --- immunohistochemistry --- syntaxin 1

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The analysis of circulating tumor cells (CTCs) as a real-time liquid biopsy approach can be used to obtain new insights into metastasis biology, and as companion diagnostics to improve the stratification of therapies and to obtain insights into the therapy-induced selection of cancer cells. In this book, we will cover all the different facets of CTCs to assemble a huge corpus of knowledge on cancer dissemination: technologies for their enrichment, detection, and characterization; their analysis at the single-cell level; their journey as CTC microemboli; their clinical relevance; their biology with the epithelial-to-mesenchymal transition (EMT); their stem-cell properties; their potential to initiate metastasis at distant sites; their ex vivo expansion; and their escape from the immune system.

n/a --- FOLFIRINOX --- immunofluorescence imaging --- AR-V7 --- circulating tumour cells --- chemoradioresistance --- CTC-based treatment decisions --- rVAR2 --- immunophenotyping --- immune system --- CellSearch® --- flow cytometry --- clinical trials --- circulating tumor DNA --- synaptophysin --- stem cells --- colorectal cancer --- melanoma --- CTC biology --- platelets --- AR --- CTC capture technology --- castration resistant prostate cancer (CRPC) --- PD-L1 expression --- rovalpituzumab tesirine --- HMB-45 --- thymidylate synthase --- ctDNA --- tumor cell dissemination --- solid cancers --- metastasis --- locally advanced rectal cancer --- miRNA --- epithelial-to-mesenchymal transition (EMT) --- NSCLC --- tumor biomarkers --- tumor stem cells --- circulating tumor cells --- major histocompatibility complex class I (MHCI) --- bone marrow --- heterogeneity --- cerebrospinal liquid biopsy --- fish --- glioma --- in vivo flow cytometry --- colorectal surgery --- CellSearch --- single-cell analysis --- disseminated tumor cells --- EasyCount slides --- microsatellite instability --- circulating plasma cells --- circulating leukemia cells --- ARV7 --- SLUG --- androgen receptor --- metastatic colorectal cancer --- leukocyte-derived extracellular vesicles --- prostate cancer (PCa) --- neutrophils --- liquid biopsy --- enzalutamide --- CD133 --- enrichment and detection technologies --- biomarkers --- immune checkpoint inhibitors --- biomarker --- RAD23B --- microbiome --- integrin B1 --- ACCEPT --- emboli --- small-cell lung carcinoma --- EPISPOT --- microfluidics --- early breast cancer --- circulating tumor cells (CTC) --- tumor-initiating cells (TICs) --- immunomodulation --- xenograft models --- CTC-derived xenografts --- malaria --- circulating tumor cells (CTCs) --- clinical utility --- exosomes --- liquid surgery --- ctRNA --- CTCs --- epithelial–mesenchymal transition --- targeted therapy --- hematological cells --- gene expression analysis --- hepatocellular carcinoma (HCC) --- breast cancer --- EpCAM enrichment --- prostate cancer --- CTC --- abiraterone --- fibronectin --- CTC-derived ex vivo models --- CTMat --- chromogranin A --- CTM --- exosome --- epithelial-mesenchymal transition

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Cyanobacterial abundance has increased disproportionately, and this trend is likely to continue in the coming decades. This increase not only has deleterious effects on ecosystem biodiversity but also adversely affects drinking water supplies, livestock watering, crop yields, aquaculture, etc. Thus, the proliferation of cyanobacterial blooms presents human and animal health risks due to the common production of potent toxins, cyanotoxins. Moreover, these risks are aggravated by the accumulation potential of cyanotoxins and their transference to the food chain. In spite of the worldwide increasing occurrence of cyanotoxins, they are still underestimated in regulations. However, risk management of cyanotoxins is only possible after a thorough risk evaluation, and for that purpose, toxicity and exposure data are required. Thus, occurrence and monitoring information is of key importance, and new data in relation to the conditions that favor cyanobacterial growth and cyanotoxin production are welcome in order to prevent their appearance. On the other hand, in regard to toxicity, there are still many data gaps to fill. This book compiles 10 research papers and a review, which provide valuable contributions on all these aspects and demonstrate the importance of cyanobacteria toxins research.

Research & information: general --- Environmental economics --- inflammatory bowel disease --- dextran sulfate sodium --- colitis --- microcystin --- colon --- HAB --- Adda --- canine intoxication --- MMPB --- urinalysis --- hair --- ELISA --- LC-MS/MS --- Microcystin-LR --- Non-alcoholic Fatty Liver Disease --- No Observed Adverse Effect Level --- Leprdb/J mice --- hepatotoxicity --- oxidative stress --- TiO2 enriched phosphopeptides --- Cyanotoxins --- microcystin congeners --- MC-LA --- nutrients --- climate --- Great Lakes --- raw water intake --- multivariate statistics --- long-term monitoring --- cyanobacteria --- microcystin-LR --- cylindrospermopsin --- cyanotoxins mixture --- plant growth --- toxin bioaccumulation --- cyanotoxin --- structure --- PP2A inhibition --- liquid chromatography --- mass spectrometry --- cyanotoxins --- metal --- zinc --- Microcystis aeruginosa --- in vivo --- genotoxicity --- micronucleus --- comet assay --- enzyme-modified comet assay --- rats --- drinking water quality --- Mozambique --- public health --- Llayta --- Nostoc --- flow cytometry --- liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) --- risk assessment --- management strategies --- modelling --- inflammatory bowel disease --- dextran sulfate sodium --- colitis --- microcystin --- colon --- HAB --- Adda --- canine intoxication --- MMPB --- urinalysis --- hair --- ELISA --- LC-MS/MS --- Microcystin-LR --- Non-alcoholic Fatty Liver Disease --- No Observed Adverse Effect Level --- Leprdb/J mice --- hepatotoxicity --- oxidative stress --- TiO2 enriched phosphopeptides --- Cyanotoxins --- microcystin congeners --- MC-LA --- nutrients --- climate --- Great Lakes --- raw water intake --- multivariate statistics --- long-term monitoring --- cyanobacteria --- microcystin-LR --- cylindrospermopsin --- cyanotoxins mixture --- plant growth --- toxin bioaccumulation --- cyanotoxin --- structure --- PP2A inhibition --- liquid chromatography --- mass spectrometry --- cyanotoxins --- metal --- zinc --- Microcystis aeruginosa --- in vivo --- genotoxicity --- micronucleus --- comet assay --- enzyme-modified comet assay --- rats --- drinking water quality --- Mozambique --- public health --- Llayta --- Nostoc --- flow cytometry --- liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) --- risk assessment --- management strategies --- modelling