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This book focuses on polyphenols in the Mediterranean diet, providing a detailed overview of their chemical structure, extraction and analysis methods, and their role in the diet and in flavor. Phenols are important not only in terms of preventing a number of diseases due to their antimicrobial and antioxidant effects, but also in shaping our perception of foods. The first chapter discusses consumers’ sensory assessment of foods containing polyphenols in terms of flavor and color, as well as the chemical properties and natural sources of phenolic compounds. The second chapter examines hygiene and safety claims with respect to naturally occurring polyphenols, especially in connection with organoleptic features. The third and final chapter examines the dietary sources of these molecules from various fruits, including processed products such as infusions, wines, oils and olives. Given its scope, this book is a valuable resource for researchers in academia and industry interested in food safety, hygiene and production issues related to the Mediterranean diet.

Food—Biotechnology. --- Nutrition   . --- Plant biochemistry. --- Food Science. --- Nutrition. --- Plant Biochemistry. --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Botany --- Phytochemicals --- Plant biochemical genetics --- Alimentation --- Food --- Nutrition --- Health --- Physiology --- Diet --- Dietetics --- Digestion --- Food habits --- Malnutrition --- Health aspects --- Polyphenols. --- Polyhydroxy phenols --- Polyhydroxylated phenols --- Phenols

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This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field. .

Plant physiology. --- Plants—Development. --- Botanical chemistry. --- Plant genetics. --- Plant Physiology. --- Plant Development. --- Plant Biochemistry. --- Plant Genetics. --- Botany --- Plants --- Physiology --- Genetics --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Phytochemicals --- Plant biochemical genetics

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This book encompasses the current knowledge of plant microbiomes and their potential biotechnological application for plant growth, crop yield and soil health for sustainable agriculture. The plant microbiomes (rhizospheric, endophytic and epiphytic) play an important role in plant growth, development, and soil health. Plant and rhizospheric soil are a valuable natural resource harbouring hotspots of microbes, and it plays critical roles in the maintenance of global nutrient balance and ecosystem function. The diverse group of microbes is key components of soil–plant systems, where they are engaged in an intense network of interactions in the rhizosphere/endophytic/phyllospheric. The rhizospheric microbial diversity present in rhizospheric zones has a sufficient amount of nutrients release by plant root systems in form of root exudates for growth, development and activities of microbes. The endophytic microbes are referred to those microorganisms, which colonize in the interior of the plant parts, viz root, stem or seeds without causing any harmful effect on host plant. Endophytic microbes enter in host plants mainly through wounds, naturally occurring as a result of plant growth, or through root hairs and at epidermal conjunctions. Endophytes may be transmitted either vertically (directly from parent to offspring) or horizontally (among individuals). The phyllosphere is a common niche for synergism between microbes and plant. The leaf surface has been termed as phyllosphere and zone of leaves inhabited by microorganisms as phyllosphere. The plant part, especially leaves, is exposed to dust and air currents resulting in the establishments of typical flora on their surface aided by the cuticles, waxes and appendages, which help in the anchorage of microorganisms. The phyllospheric microbes may survive or proliferate on leaves depending on extent of influences of material in leaf diffuseness or exudates. The leaf diffuseness contains the principal nutrients factors (amino acids, glucose, fructose and sucrose), and such specialized habitats may provide niche for nitrogen fixation and secretions of substances capable of promoting the growth of plants.

Agriculture. --- Microbiology. --- Microbial ecology. --- Plant breeding. --- Plant biochemistry. --- Applied Microbiology. --- Microbial Ecology. --- Plant Breeding/Biotechnology. --- Plant Biochemistry. --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Botany --- Phytochemicals --- Plant biochemical genetics --- Crops --- Agriculture --- Breeding --- Environmental microbiology --- Microorganisms --- Ecology --- Microbiology --- Microbial biology --- Biology --- Farming --- Husbandry --- Industrial arts --- Life sciences --- Food supply --- Land use, Rural --- Plants --- Bacteriology

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This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. .

Biotechnology --- Methodology. --- Chemical engineering --- Genetic engineering --- Biomaterials. --- Nucleic acids. --- Biology—Technique. --- Biochemistry. --- Botanical chemistry. --- Bioinformatics. --- Nucleic Acid. --- Biological Techniques. --- Chemical Biology. --- Plant Biochemistry. --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Botany --- Phytochemicals --- Plant biochemical genetics --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Polynucleotides --- Biomolecules --- Data processing --- Composition

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The generation of well-defined nanoparticles of excellent size and shape involves physical and chemical methodologies that are complicated, expensive, and produce hazardous toxic waste that is harmful to the environment and to human health. In order to combat the disadvantages of these methods, scientists have created “the biological method,” a new synthetic methodology that serves as a proper alternative to physical and chemical methodologies because of its easy utility, low cost, rapid synthesis, controlled size characteristics, controlled toxicity, and eco-friendliness. Nanobiotechnology is the science in which living matter can be manipulated and exploited to produce materials within the nano-scale. It is a multidisciplinary field of science framed by biology, chemistry, engineering, materials, and life sciences. Different biological entities can be exploited to yield biologically synthesized nanomaterials including bacteria, actinomycetes, yeast, fungi, viruses, algae, plant extracts, and agro-industrial waste extracts. This book represents a comprehensive review concerning the state of the art in nanobiotechnology, emphasizing the use of diverse biological entities in the science, and its versatile applications. It describes currently existing methodology with the latest published references, and provides safety information. It serves as the ideal guide for scientists interested in exploring nanobiotechnology.

Nanobiotechnology. --- Bionanotechnology --- Biotechnology --- Nanotechnology --- Plant breeding. --- Nanotechnology. --- Microbiology. --- Plant biochemistry. --- Aquatic ecology . --- Plant Breeding/Biotechnology. --- Plant Biochemistry. --- Freshwater & Marine Ecology. --- Aquatic biology --- Ecology --- Phytochemistry --- Plant biochemistry --- Plant chemistry --- Biochemistry --- Botany --- Phytochemicals --- Plant biochemical genetics --- Microbial biology --- Biology --- Microorganisms --- Molecular technology --- Nanoscale technology --- High technology --- Crops --- Agriculture --- Breeding --- Ultraestructura (Biologia) --- Nanobioingenieria --- Nanobiologia --- Nanobiotecnologia --- Ultraestructura cel·lular --- Cèl·lules --- Microestructura --- Citologia --- Histologia