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This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.

Proteins. --- Biological transport. --- Cell membranes. --- Protein Biochemistry. --- Membrane Trafficking. --- Cell surfaces --- Cytoplasmic membranes --- Plasma membranes --- Plasmalemma --- Membranes (Biology) --- Glycocalyces --- Membrane transport --- Passive transport, Biological --- Physiological transport --- Transport, Biological --- Diffusion --- Osmosis --- Proteids --- Biomolecules --- Polypeptides --- Proteomics

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This book discusses unique ion channels and transporters that are located within epithelial tissues of various organs including the kidney, intestine, pancreas and respiratory tract. As the authors show, these channels and transporters play crucial roles in transepithelial ion and fluid transport across epithelia and their contribution to maintaining homeostasis. Readers will be introduced to the fundamentals of ion transport in terms of function, modelling, regulation, structure and pharmacology. This is the first of three volumes highlighting the importance of epithelial ion channels and transporters in basic physiology and pathophysiology of human diseases. This volume focuses on basic fundamentals of epithelial transport physiology. There is a range of chapters dedicated to specific aspects of epithelial ion transport and cell function. Accordingly, the authors discuss techniques used to determine epithelial function, principles of epithelia transport, polarization of epithelial cells, mathematical modelling of epithelial ion transport, protein folding of ion channels, degradation epithelial ion channels, fundamentals of epithelial sodium, potassium and chloride transport, fundamentals of bicarbonate secretion, volume regulation, and microRNA regulation of epithelial channels and transporters. Given its scope, Volume 1 offers a valuable resource for physiology students, scientists and clinicians alike. .

Human physiology. --- Molecular biology. --- Proteins . --- Cell membranes . --- Human Physiology. --- Molecular Medicine. --- Protein Science. --- Membrane Biology. --- Cell surfaces --- Cytoplasmic membranes --- Plasma membranes --- Plasmalemma --- Membranes (Biology) --- Glycocalyces --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Systems biology --- Human biology --- Medical sciences --- Physiology --- Human body

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This book covers different omics aspects related to the extracellular matrix (ECM), namely specific omics resources focused on the extracellular matrix (e.g., databases, repositories and atlases), quantitative proteomics applied to specific extracellular matrices (e.g. basement membranes), biological processes such as ECM degradation (degradomics), cell-matrix interactions (adhesomes), signaling pathways, biomarker discovery and diseases, and interactomics (extracellular matrix interaction networks including not only protein-protein but also protein-glycosaminoglycan interactions). The volume also includes recent advances in glycomics and glycobioinformatics applied to proteoglycans and glycosaminoglycans, which are key biological players. The use of omics data to build dynamic models of ECM-regulated biological pathways is addressed, together with the requirement to standardize omic data, which is a prerequisite for the FAIR (Findability, Accessibility, Interoperability, and Reusability) guiding principles for scientific data management. This book will be of great interest to a broad readership from beginners to advanced researchers, who are interested in extracellular matrix omics and will inspire future research topics. .

Bioinformatics. --- Cell membranes . --- Analytical chemistry. --- Human physiology. --- Membrane Biology. --- Analytical Chemistry. --- Human Physiology. --- Human biology --- Medical sciences --- Physiology --- Human body --- Analysis, Chemical --- Analytic chemistry --- Chemical analysis --- Chemistry, Analytic --- Chemistry --- Cell surfaces --- Cytoplasmic membranes --- Plasma membranes --- Plasmalemma --- Membranes (Biology) --- Glycocalyces --- Bio-informatics --- Biological informatics --- Biology --- Information science --- Computational biology --- Systems biology --- Data processing --- Cell membranes.

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This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.

Proteïnes de membrana --- Biofísica --- Proteïnes de membranes --- Membranes (Biologia) --- Proteïnes --- Connexines --- Proteïnes G --- Proteïnes SNARE --- Transportadors ABC --- Biologia física --- Física biològica --- Biologia --- Física --- Absorció (Fisiologia) --- Biologia molecular --- Biònica --- Biomagnetisme --- Biomecànica --- Enginyeria biomèdica --- Física mèdica --- Interfícies biològiques --- Reologia (Biologia) --- Biological transport. --- Cell membranes. --- Biophysics. --- Proteins. --- Membrane Trafficking. --- Protein Biochemistry. --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Biological physics --- Biology --- Medical sciences --- Physics --- Cell surfaces --- Cytoplasmic membranes --- Plasma membranes --- Plasmalemma --- Membranes (Biology) --- Glycocalyces --- Membrane transport --- Passive transport, Biological --- Physiological transport --- Transport, Biological --- Diffusion --- Osmosis

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This book discusses unique ion channels and transporters that are located within epithelial tissues of various organs including the kidney, intestine, pancreas and respiratory tract. The authors will show, that each of these channels and transporters play crucial roles in transepithelial ion and fluid transport across epithelia and their responsibility in maintaining homeostasis. The reader gains an understanding of the fundamentals of epithelial ion transport, in terms of function, modelling, regulation, trafficking, structure and pharmacology. This is the third of three volumes highlighting the importance of epithelial ion channels and transporters in basic physiology and pathophysiology of human diseases. The focus of this volume lies with different ion channel and transporter families. Additionally, this volume benefits from pharmaceutical contributors and their insights into recent pre-clinical drug discovery efforts and results from clinical trials. Overall, these chapters offer a more thorough coverage of individual epithelial ion channels and transporters from the 1st Edition, along with eleven new chapters. That makes Volume 3 an insightful contribution for physiology students, scientists and clinicians.

Human physiology. --- Molecular biology. --- Proteins . --- Cell membranes . --- Human Physiology. --- Molecular Medicine. --- Protein Science. --- Membrane Biology. --- Cell surfaces --- Cytoplasmic membranes --- Plasma membranes --- Plasmalemma --- Membranes (Biology) --- Glycocalyces --- Proteids --- Biomolecules --- Polypeptides --- Proteomics --- Molecular biochemistry --- Molecular biophysics --- Biochemistry --- Biophysics --- Systems biology --- Human biology --- Medical sciences --- Physiology --- Human body --- Epithelium. --- Tissues --- Cilia and ciliary motion --- Epiteli --- Canals iònics --- Farmacologia --- Medicina --- Biofarmàcia --- Biotransformació (Metabolisme) --- Dermofarmacologia --- Desenvolupament de medicaments --- Estabilitat dels medicaments --- Farmacocinètica --- Farmacogenètica --- Farmacologia cardiovascular --- Farmacologia clínica --- Farmacologia dental --- Farmacologia geriàtrica --- Farmacologia molecular --- Farmacologia obstètrica --- Farmacologia ocular --- Farmacologia pediàtrica --- Farmacologia respiratòria --- Farmacologia renal --- Farmacologia veterinària --- Immunofarmacologia --- Isòtops radioactius en farmacologia --- Neurofarmacologia --- Psicofarmacologia --- Química farmacèutica --- Radiofàrmacs --- Resistència als medicaments --- Toxicologia --- Medicaments --- Quimioteràpia --- Canals de calci --- Canals de clorur --- Canals de potassi --- Teixits (Histologia) --- Cèl·lules epitelials --- Endoteli --- Epidermis --- Membrana mucosa --- Fotofarmacologia